imported>Stuart S. Levine: Created page with "''' PRICING UPDATE 7/1/2024 ''' == SEQUENCING == Please note that samples submitted from '''CORE LAB'''s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQ SEQUENCING === {| class="wikitable" border=1 ! NOVASEQ SEQUENCING !width=80| CORE LAB !width=80| MIT !width=80| Non-MIT !width=80| uni..."

2024-06-12T02:16:13Z

Created page with "''' PRICING UPDATE 7/1/2024 ''' == SEQUENCING == Please note that samples submitted from '''CORE LAB'''s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason. === NOVASEQ SEQUENCING === {| class="wikitable" border=1 ! NOVASEQ SEQUENCING !width=80| CORE LAB !width=80| MIT !width=80| Non-MIT !width=80| uni..."

New page

''' PRICING UPDATE 7/1/2024 '''

== SEQUENCING ==
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason. 

=== NOVASEQ SEQUENCING ===
{| class="wikitable" border=1
! NOVASEQ SEQUENCING
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
|NovaSeq SP 100nt
|align=right| $3,699
|align=right| $4,068
|align=right| $5,178
|rowspan=9| Per flowcell
|rowspan=9| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|NovaSeq SP 300nt**
|align=right| $5,238
|align=right| $5,761
|align=right| $7,333.20
|-
|NovaSeq SP 500nt
|align=right| $7,020
|align=right| $7,722
|align=right| $9,828
|-
|NovaSeq S1 100nt**
|align=right| $5,832
|align=right| $6,415.20
|align=right| $8,164.80
|-
|NovaSeq S1 200nt**
|align=right| $7,452
|align=right| $8,197.20
|align=right| $10,432
|-
|NovaSeq S1 300nt**
|align=right| $8,127
|align=right| $8,939.70
|align=right| $11,377.80
|-
|NovaSeq S2 100nt**
|align=right| $10,395
|align=right| $11,434.50
|align=right| $14,553
|-
|NovaSeq S2 200nt**
|align=right| $12,960
|align=right| $14,256
|align=right| $18,144
|-
|NovaSeq S2 300nt**
|align=right| $13,500
|align=right| $14,850
|align=right| $18,900
|-
|NovaSeq S4 50nt
|align=right| $3,780
|align=right| $4,158
|align=right| $5,292
|rowspan=2| Per Lane
|rowspan=2| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|NovaSeq S4 300nt
|align=right| $5,400
|align=right| $5,940
|align=right| $7,560
|-
|}
** - minimal inventory maintained

=== NEXTSEQ500 SEQUENCING ===
{| class="wikitable" border=1
! NEXTSEQ SEQUENCING
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
|91nt kit (eg. 40PE or 75SE)
|align="right"| $2,570
|align="right"| $2,827
|align="right"| $3,598
|align="center" rowspan="3"| per flowcell
|rowspan="3"| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|166nt kit (eg. 80PE or 150SE)
|align="right"| $4,375
|align="right"| $4,812.50
|align="right"| $6,125
|-
|316nt kit (eg. 150PE or 300SE)
|align="right"| $6,420
|align="right"| $7,062
|align="right"| $8,988
|}

=== MISEQ SEQUENCING ===
{| class="wikitable" border=1
! MISEQ SEQUENCING
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
|70nt V2 kit (eg. 30PE or 70SE)
|align="right"| $1,320
|align="right"| $1,452
|align="right"| $1,848
|align="center" rowspan=6| per lane
|rowspan=6| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|150nt V3 kits (eg. 75PE)
|align="right"| $1,430
|align="right"| $1,573
|align="right"| $2,002
|-
|300nt V2 kit (eg. 150PE)
|align="right"| $1,595
|align="right"| $1,754.50
|align="right"| $2,233
|-
|500nt V2 kit (eg. 250PE)
|align="right"| $1,760
|align="right"| $1,936
|align="right"| $2,464
|-
|600nt V3 kit (eg. 300PE)
|align="right"| $2,200
|align="right"| $2,420
|align="right"| $3,080
|-
|500nt '''NANO''' Run (eg. 250PE)
|align="right"| $770
|align="right"| $847
|align="right"| $1,078
|}

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===
{| class="wikitable" border=1
! SINGULAR G4 SEQUENCING
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
|50 PE: F3 Lane (~100m reads)
|align="right"| $300
|align="right"| $330
|align="right"| $420
|align="center" rowspan=2| per lane
|rowspan=3| Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
|150 PE: F3 Lane (~100m reads)
|align="right"| $450
|align="right"| $495
|align="right"| $630
|-
|Rapid 300nt: F3 flowcell (~400m reads)*
|align="right"| $1800
|align="right"| $1980
|align="right"| $2520
|align="center"| per flowcell
|-
|}
* Rapid flowcells are required for adapted Illumina libraries.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI] ===
{| class="wikitable" border=1
! ELEMENT AVITI SEQUENCING
!width=80| Lane/Flowcell
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
|rowspan=2| 75 PE
| Full flowcell (2 lane, 800m read)
|align="right"| $1,900
|align="right"| $2,090
|align="right"| $2,660
|align="center"| per flowcell
|rowspan=5| Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
|One lane (~400m read)
|align="right"| $1,000
|align="right"| $1,100
|align="right"| $1,400
|align="center"| per lane
|-
|rowspan=2| 150 PE
| Full flowcell (2 lane, ~800m read)
|align="right"| $2,650
|align="right"| $2,915
|align="right"| $3,710
|align="center"| per flowcell
|-
|One lane (~400m read)
|align="right"| $1,375
|align="right"| $1,513
|align="right"| $1,925
|align="center"| per lane
|-
| 300 PE
| Full flowcell (~200m reads)
|align="right"| $3,500
|align="right"| $3,850
|align="right"| $4,900
|align="center"| per lane
|}

=== OTHER SHORT READ SERVICES ===
{| class="wikitable" border=1
! ILLUMINA SUPPORTING SERVICES
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
| Quality Control Only -only one QC is included per sequencing lane.
|align="right"| $35
|align="right"| $38.50
|align="right"| $49
|align="center"| per sample
| Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling.
|align="right"| $15
|align="right"| $16.50
|align="right"| $21
|align="center"| per sample
| Includes Fluorometric quantification and Frag.Analyzer
|-
|qPCR only (short read)
|align="right"| $25
|align="right"| $27.50
|align="right"| $35
|align="center"| per sample
| Includes qPCR values from standard QC only
|-
|qPCR only - full plate(short read)
|align="right"| $400
|align="right"| $440
|align="right"| $560
|align="center"| per sample
| excludes column 1 (used for controls)
|}

=== LONG READ SEQUENCING ===

{| class="wikitable" border=1
! PROMETHION SEQUENCING
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
| 72 hour Flowcell
|align="right"| $1,200
|align="right"| $1,320
|align="right"| $1,680
|align="center"|per Flowcell
||Fastq stored for 1y. Trace data stored only 30d.
|}

== LIBRARY GENERATION ==
=== SHORT READ LIBRARY - CONVERSION ===
{| class="wikitable" border=1
!width=100| Input library
!width=100| Convert To
!width=100| Method
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|align="center"|Illumina
|align="center"|Singular
|align="center"|Index Overwrite (per sample)
|align="right"| $30
|align="right"| $33
|align="right"| $42
| Includes QC and reindexing
|-
|align="center"|Illumina
|align="center"|Singular
|align="center"|P5/P7 conversion (per pool)
|align="right"| $100
|align="right"| $110
|align="right"| $140
| Includes QC. Samples not reindexed Requires full flowcell for sequencing (Rapid) 
|-
|align="center"|Illumina
|align="center"|Element
|align="center"|Index Overwrite (per sample)
|align="right"| $30
|align="right"| $33
|align="right"| $42
| Includes QC and reindexing - "Elevate" workflow
|-
|align="center"|Illumina
|align="center"|Element
|align="center"|P5/P7 splicing (per pool)
|align="right"| $100
|align="right"| $110
|align="right"| $140
| Includes QC. Samples not reindexed. Requires high input amounts (>10nM)
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable" border=1
!width=160| ILLUMINA Sample Preparation
!width=60| Type
!width=60| Batch Size
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
! colspan="4" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
! colspan="3" style="color: blue;" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
|rowspan=3 |LIGATION BASED NEB UltraII
|align="center"|STD
|align="center"| per sample
|align="right"| $105
|align="right"| $115
|align="right"| $157
| Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|- style="color: blue;"
|align="center" rowspan=2|HTL
|align="center"| 24
|align="right"| $900
|align="right"| $990
|align="right"| $1,260
|rowspan=2 | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|- style="color: blue;"
|align="center"| 96
|align="right"| $1,800
|align="right"| $1,980
|align="right"| $2,520
|-

|Tagmentation NEXTERA-XT
|align="center" rowspan=2|STD
|align="center" rowspan=2|per Sample
|align="right"| $105
|align="right"| $115
|align="right"| $157
| rowspan="2" | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
|Tagmentation NEXTERA-FLEX
|align="right"| $157
|align="right"| $173
|align="right"| $236

|- style="color: blue;"
|rowspan=2 | NEXTERA-XT OR FLEX
|rowspan=2 align="center"| HTL
|align="center"| ''24''
|align="right"| $900
|align="right"| $990
|align="right"| $1,260
|rowspan=2 | Includes tagmentation, indexing, enrichment, and quality control spotcheck(SYBR and FA).
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $1,800
|align="right" style="color: blue;" | $1,980
|align="right" style="color: blue;" | $2,520

|-style="color: blue;"
|rowspan="2" | 16S Amplicon
|align="center" rowspan=2 | HTL
|align="center"|48
|align="right" | $1,900
|align="right" | $2,090
|align="right" | $2,660
|rowspan="2" | Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-style="color: blue;"
|align="center"|add'l 48
|align="right" | $650
|align="right" | $715
|align="right" | $910

|-style="color: blue;"
|rowspan="2" | Other Amplicon
|rowspan=2 align="center"| HTL
|align="center" |48
|align="right" | $1,600
|align="right" | $1,760
|align="right" | $2,240
|rowspan="2" | Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|- style="color: blue;"
|align="center"|add'l 48
|align="right" | $630
|align="right" | $693
|align="right" | $945
|-
! colspan="7" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
|style="color: blue;" colspan=2| High Throughput Setup
|align="center" style="color: blue;" |48 samples
|align="right" style="color: blue;" | $60
|align="right" style="color: blue;" | $66
|align="right" style="color: blue;" | $84
|
|-
|colspan=2|Sample Cleanup
|align="center"|per sample
|align="right"| $8
|align="right"| $8.80
|align="right"| $11.20
|SPRI cleanup of samples to remove impurities.
|-
|style="color: blue;" colspan=2|Quantification
|align="center" style="color: blue;" |per sample
|align="right" style="color: blue;" | $10
|align="right" style="color: blue;" | $11
|align="right" style="color: blue;" | $14
|Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable" border=1
!width=160| ILLUMINA Sample Preparation
!width=60| Type
!width=60| Batch Size
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
! colspan="4" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
! colspan="3" style="color: blue;" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
|rowspan="3"|polyA based isolation NEB UltraII RNAseq
|align="center"|STD
|align="center"| per sample
|align="right"| $157
|align="right"| $173
|align="right"| $236
|Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
|rowspan=2 align="center" style="color: blue;"| HTL (NEB only)
|align="center" style="color: blue;" | ''24''
|align="right" style="color: blue;" | $1,500
|align="right" style="color: blue;" | $1,650
|align="right" style="color: blue;" | $2,100
|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $3,000
|align="right" style="color: blue;" | $3,300
|align="right" style="color: blue;" | $4,200
|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-

|rowspan="2"| High Throughput 3' Digital Gene Expression (HT3DGE)
|rowspan=2 align="center" style="color: blue;"| HTL
|align="center" style="color: blue;"| ''24''
|align="right" style="color: blue;" | $1,050
|align="right" style="color: blue;" | $1,155
|align="right" style="color: blue;" | $1,470
| rowspan="2" style="color: blue;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $2,100
|align="right" style="color: blue;" | $2,310
|align="right" style="color: blue;" | $2,940
|-

|rowspan="3"|Total RNA sequencing NEB RNAseq +Ribodepletion
|align="center"|STD
|align="center"| per sample
|align="right"| $235
|align="right"| $258.5
|align="right"| $352.5
|Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
|rowspan=2 align="center" style="color: blue;"| HTL (NEB only, covaris RNA fragmentation)
|align="center" style="color: blue;" | ''24''
|align="right" style="color: blue;" | $2,500
|align="right" style="color: blue;" | $2,750
|align="right" style="color: blue;" | $3,500
|rowspan=2|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $5,000
|align="right" style="color: blue;" | $5,500
|align="right" style="color: blue;" | $7,000
|-

|rowspan=3|Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex
|align="center"| '''STD''' v4
|align="center"|per sample
|align="right"| $262
|align="right"| $289
|align="right"| $394
| rowspan="3" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
|rowspan="2" align="center" style="color: blue;"| '''HTL''' v2
|align="center" style="color: blue;" | ''24''
|align="right" style="color: blue;" | $2,000
|align="right" style="color: blue;" | $2,200
|align="right" style="color: blue;" | $2,800
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $3,200
|align="right" style="color: blue;" | $3,520
|align="right" style="color: blue;" | $4,480
|-

|rowspan="3"|Low Input Mammalian Total Clontech ZapR Kit
|align="center"| STD
|align="center"| per sample
|align="right"| $210
|align="right"| $231
|align="right"| $315
| rowspan="1" | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
|rowspan=2 align="center" style="color: blue;"| HTL (covaris RNA fragmentation)
|align="center" style="color: blue;" | ''24''
|align="right" style="color: blue;" | $3000
|align="right" style="color: blue;" | $3300
|align="right" style="color: blue;" | $4200
|rowspan=2|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $5000
|align="right" style="color: blue;" | $5500
|align="right" style="color: blue;" | $7000
|-
| Small RNA
|align="center"| STD
| align="center"|per sample
| align="right"|$289
| align="right"|$318
| align="right"|$433
| Uses NEB, BIOO or QIAGEN small RNA kit
|-
! colspan="7" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
|colspan=2 style="color: blue;" | High Throughput Setup
|align="center" style="color: blue;" |48 samples
|align="right" style="color: blue;" | $60
|align="right" style="color: blue;" | $66
|align="right" style="color: blue;" | $84
|
|-
|colspan=2|Sample Cleanup
|align="center"|per sample
|align="right"| $8
|align="right"| $8.80
|align="right"| $11.20
|SPRI cleanup of samples to remove impurities.
|-
|colspan=2 style="color: blue;" |Quantification
|align="center" style="color: blue;" |per sample
|align="right" style="color: blue;" | $10
|align="right" style="color: blue;" | $11
|align="right" style="color: blue;" | $14
|Quantification of samples (FA) prior to arraying.
|-
|}

.

=== LONG READ LIBRARIES ===
{| class="wikitable" border=1
!Oxford Nanopore Sample Preparation
!width=120| Nanopore Kit
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|Standard Ligation || LSK || $160 || $176 || $224
|rowspan=6|Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
|Native Barcoding (ligation indexing) || - || $50 || $55 || $70
|-
|SMARTseq -> Ligation || Cv4+LSK || $300 || $330 || $420
|-
|Rapid Prep (tagment - ~10-20kb) || RAD || $150 || $165 || $210
|-
|Long Insert Prep || RAD (modified, target >100kb) || $262 || $289 || $394
|-
|Direct RNA || RNA004 || $160 || $176 || $225
|}

== SAMPLE EXTRACTION ==
=== CHEMAGIC360 ===
{| class="wikitable" border=1
!TYPE
!width=120| Batch Size
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|DNA
|96
|$700
|$770
|$980
|
|-
|RNA
|96
|$800
|$880
|$1120
|
|}

== SINGLE CELL & SPATIAL SERVICES ==
=== 10X CHROMIUM ===
{| class="wikitable" border=1
! 10X USAGE
!width=120| Batch Size
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|colspan=6 align="center"|'''Assisted Service'''
|-
|colspan=6 |''DIGITAL GENE EXPRESSION''
|-
|rowspan=2 |5' RNA 3' RNA
|setup || $1,015 || $1,116.50 || $1,421 || charge per submission
|-
|per sample|| $1,750 || $1,925 || $2,450 || Includes QC of cDNA and creation of Short Read Library
|-
|rowspan=2 |Fixed RNA probe based. human/mouse only
|setup || $1300 || $1430 || $1820 || charge per submission
|-
|per sample (4plex) || $3900|| $4290 || $5460 || Includes reagents for hashing of 4 samples in one channel. QC of cDNA and creation of Short Read Library
|-
|rowspan=2 |Amplicon (CITE-SEQ, TCR, BCR, antibody barcocde, etc // per amplicon)
|setup || $470 || $517 || $658 || additional prep beyond DGE
|-
|per sample|| $45 || $50 || $63 || Includes QC of cDNA and creation of Short Read Library
|-
|colspan=6 |''scATACseq''
|-
|rowspan=2 |ATACseq
|setup || $880 || $968 || $1,232 || charge per submission
|-
|per sample|| $1,675|| $1,843|| $2,345|| Includes QC and creation of Short Read Library
|-
|colspan=6 |''Multiome (ATAC+3'RNA)''
|-
|rowspan=2 |MULTI
|setup || $1,870|| $2,057|| $2,618|| charge per submission
|-
|per sample|| $3,100|| $3,410|| $4,340|| Includes QC of cDNA and creation of Short Read Library
|-
|colspan=6 |''CANCELLATION''
|-
|per session || || $200|| $220|| $280|| <24h notice
|}
*please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== NAMOCELL ===
{| class="wikitable" border=1
! NAMOCELL
!width=120| Batch Size
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|Usage || Per run || $20 || $22 || $30 || per hour
|-
|Cartridge|| 1 cartridge per sample|| $60 || $66 || $90 ||
|}

=== VISIUM ===
{| class="wikitable" border=1
! SLIDES
!width=120| Batch Size
!width=80| KI Only
!width=80| [[BioMicroCenter:CoreDeps|Other CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|Test Array ||slide|| $1,300|| $1,511|| $1,607|| $2435|| recommended 1x per tissue type for fresh frozen
|-
|Sample Slide - Fresh Frozen ||1 slide / 4 tiles|| $6,210|| $6,340|| $6,940|| $9,067 || Includes H&E stain images + Library Generation
|-
|Sample Slide - FFPE (hs/mm only) ||1 slide / 2 tiles|| $4,550|| $4,550|| $4,996|| $6,359 || Includes library generation only.
|-
|Sample Slide - HD (hs/mm only) ||1 slide / 2 tiles|| $7,850|| $7,850|| $8,596|| $10,940 || Includes library generation only.
|}

== OTHER GENOMICS SERVICES ==

 

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===
{| class="wikitable" border=1
! QUALITY CONTROL
!width=100| unit
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=100| MIT
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|Fragment Analyzer: RNA or DNA
|per sample ||$15 || $16.50 || $21
|
|-
|Fragment Analyzer: Preloaded row
| per row (12) ||$80||$88 || $112
| 2ul per sample in each well using plates from BMC
|-
|FemtoPulse: RNA or DNA
|per sample || $30 || $33 || $42
|
|-
|FemtoPulse: Preloaded Row
|per row (12) || $160 || $176 || $224
| 2ul per sample in each well using plates from BMC
|-
|BioAnalyzer: Walkup Service
|per sample || $50 || $55 || $70
|
|-
|qPCR only (Illumina quantification test)
|per sample || $25 || $27.50 || $35
| 4 point serial dilution
|-
|Full plate qPCR (Illumina quantification test)
|per plate || $400 || $440 || $560
| full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
|}

=== OLIGO SYNTHESIS ===
{| class="wikitable" border=1
!Platform
!width=100| Service
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=100| MIT
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|rowspan=3| Dr.Oligo
|Assisted
|$2000 +consumables
|$2200 +consumables
|$2800 +consumables
|includes setup and run of the instrument only. Large reagents / N2 included.
|-
|Walkup
|$1000 +consumables
|$1100 +consumables
|NA
|includes run of the instrument only. Large reagents / N2 included.
|-
|Training
|$4000
|$4400
|NA
|ncludes two instrument runs.
|-
|rowspan=6| DNAscript Syntax
|Setup (per run)
|$1000
|$1100
|$1400
|Per run cost
|-
|2000 nucleotide synthesis
|$500
|$550
|$700
|Up to 96 samples
|-
|8000 nucleotide synthesis
|$1500
|$1650
|$2100
|Up to 96 samples
|-
|add Biotin (max48)
|$115
|$126
|$161
|rowspan = 3| max 48 per run and 2 per oligo. Couple to T only.
|-
|add Fluor
|$300
|$330
|$420
|-
|add quench
|$100
|$110
|$140
|}

== [[BioMicroCenter:RTPCR|REAL TIME PCR]] ==

{| border=1
! OTHER LAB SERVICES
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=100| unit
! SIGN UP
! Notes
|-
| Roche LightCycler 480
|align="right"| $15
|align="right"| N/A
|align="center"| per plate
| [[BioMicroCenter:Forms#Calenders|CALENDAR]] *available for CORE only
| Contact [[BioMicroCenter:People|Christopher Hallee]] for training
|-
| SYBR Green (KAPA/Roche)
|align="right"| $45
|align="right"| N/A
|align="center"| per ml
|
| Available for MIT only
|-
| 96-well PLATES
|align="right"| $10
|align="right"| N/A
|align="center"| per plate
|
| Available for MIT only
|-
|}
.
 

== OTHER LAB SERVICES ==
{| border=1
! OTHER LAB SERVICES
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=100| unit
! SIGN UP
! Notes
|-
|colspan=6 align="center"| '''COVARIS SONICATOR'''
|-
|COVARIS E220
|align="right"|$20
|align="right"|$30
|per hour
|[https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar]
|tubes available for purchase.
|-

| colspan="6" align="center"| '''PIPPIN PREP'''
|-
|Gel Isolation
|align="right"| $75
|align="right"| $105
|align="center"| per gel (5 lanes)
| TBA
|
|-
|Training
|align="right"| $25
|align="right"| $50
|align="center"| per session
| TBA
| Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" align="center"| '''PLATE READER'''
|-
| Varioskan
|align="right"| $18
|align="right"| $27
|align="center"| per hour
| [[BioMicroCenter:Forms#Calenders|CALENDAR]]
| Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" align="center"| '''ROBOTICS'''
|-
| Tecan EVO 150
|align="right"| $75
|align="right"| $82.5
|align="center"| per 1h block - based on scheduled time.
| [[BioMicroCenter:Forms#Calenders|CALENDAR]]
|
|-
|non-filter tips
|align="right"| $8
|align="right"| $8.80
|align="center"| per box
|
|** Please let us know if there is additional plasticware you would like us to stock.
|-
|Filter tips
|align="right"| $12.00
|align="right"| $13.20
|align="center"| per box
|
|
|-
| Training
|align="right"| $65
|align="right"| $71.5
|align="center"| per hour
| Contact [[BioMicroCenter:People|Tatum Murdock]]
|
|-
| colspan="6" align="center"| '''NANODROP'''
|-
| Nanodrop
|align="right"| --
|align="right"| --
|align="center"|
| Walk up service
| * no charge
|-
|}
 

== COMPUTATIONAL SERVICES ==
{| class="wikitable" border=1
! COMPUTATIONAL SERVICES
!width=200| unit
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=100| Other MIT / non-MIT
! Notes
|-
| Informatics Support
| align="right"| per hour
| align="right"| $90
| align="right"| $125 / $180
| Very limited support for non-MIT users
|-
| Informatics Share
| align="right"| per 0.1FTE
| align="right"| $1,250
| align="right"| N/A
| MIT only. Priority access to informatics.
|-
|Ingenuity Pathway Analysis
|align="right"|per dataset
|align="right" | $100
|align="right" | $110/$150
| Recovery of licence cost.
|-
|Active Data storage
|align="right"| per TB per year
|align="right"| $100
|align="right"| $300 / N/A
|
|-
| Tape Archive / Recovery
|align="right"| per tape
|align="right"| $60
|align="right"| $70
|
|-
| Informatics Training Classes
|align="right"| per session
|align="right"| $50
|align="right"| $100
|
|}

BioMicroCenter:PricingFY2025 - Revision history