imported>Stuart S. Levine: /* LONG READ SEQUENCING */

2021-06-16T12:26:41Z

LONG READ SEQUENCING

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{{BioMicroCenter}}
== SEQUENCING ==
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason. 

''' PRICING UPDATE 7/1/2021 '''

=== NOVASEQ SEQUENCING ===
pricing update effective 10/1/2020
{| class="wikitable" border=1
! NOVASEQ SEQUENCING
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
|NovaSeq: 400m reads
|align=right| $1,600
|align=right| $1,760
|align=right| $2,240
|Per 400m reads (max 150PE)
|Requires BMC library prep, Max 96 samples
|-
|NovaSeq SP 100nt
|align=right| $3,365
|align=right| $3,701
|align=right| $4,711
|rowspan=9| Per flowcell
|rowspan=9| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|NovaSeq SP 300nt**
|align=right| $5,200
|align=right| $5,720
|align=right| $7,280
|-
|NovaSeq SP 500nt
|align=right| $6,400
|align=right| $7,040
|align=right| $8,960
|-
|NovaSeq S1 100nt**
|align=right| $5,300
|align=right| $5,830
|align=right| $7,420
|-
|NovaSeq S1 200nt**
|align=right| $6,900
|align=right| $7,590
|align=right| $9,660
|-
|NovaSeq S1 300nt**
|align=right| $7,750
|align=right| $8,525
|align=right| $10,850
|-
|NovaSeq S2 100nt**
|align=right| $9,500
|align=right| $10,450
|align=right| $13,300
|-
|NovaSeq S2 200nt**
|align=right| $11,500
|align=right| $12,650
|align=right| $16,100
|-
|NovaSeq S2 300nt**
|align=right| $12,600
|align=right| $13,860
|align=right| $17,640
|-
|NovaSeq S4 50nt
|align=right| $3,500
|align=right| $3,850
|align=right| $4,900
|rowspan=2| Per Lane
|rowspan=2| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|NovaSeq S4 300nt
|align=right| $5,000
|align=right| $5,500
|align=right| $7,000
|-
|}
** - minimal inventory maintained

=== NEXTSEQ500 SEQUENCING ===
{| class="wikitable" border=1
! NEXTSEQ SEQUENCING
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
|91nt kit (eg. 40PE or 75SE)
|align="right"| $2,270
|align="right"| $2,497
|align="right"| $3,178
|align="center" rowspan="3"| per flowcell
|rowspan="3"| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|166nt kit (eg. 80PE or 150SE)
|align="right"| $3,900
|align="right"| $4,290
|align="right"| $5,460
|-
|316nt kit (eg. 150PE or 300SE)
|align="right"| $5,800
|align="right"| $6,380
|align="right"| $8,120
|}

=== MISEQ SEQUENCING ===
{| class="wikitable" border=1
! MISEQ SEQUENCING
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
|70nt V2 kit (eg. 30PE or 70SE)
|align="right"| $1,250
|align="right"| $1,375
|align="right"| $1,750
|align="center" rowspan=6| per lane
|rowspan=6| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|150nt V3 kits (eg. 75PE)
|align="right"| $1,350
|align="right"| $1,485
|align="right"| $1,890
|-
|300nt V2 kit (eg. 150PE)
|align="right"| $1,475
|align="right"| $1,623
|align="right"| $2,100
|-
|500nt V2 kit (eg. 250PE)
|align="right"| $1,703
|align="right"| $1,874
|align="right"| $2,310
|-
|600nt V3 kit (eg. 300PE)
|align="right"| $2,043
|align="right"| $2,248
|align="right"| $2,898
|-
|500nt '''NANO''' Run (eg. 250PE)
|align="right"| $730
|align="right"| $803
|align="right"| $1,022
|}

=== HISEQ2000 SEQUENCING ===
The HiSeq2000 in the BioMicro Center is in the process of being retired. We strongly encourage users to move to one of the other sequencers if possible. 
{| class="wikitable" border=1
! HISEQ SEQUENCING
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
|Single End 40nt
|align="right"| $1,200
|align="right"| $1,320
|align="right"| $1,800
|align="center"| per lane
| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1y.
|-
| Other read lengths
|colspan="5" align=left |7/1/18: Custom only. Requires purchase of full flowcell.
|}

=== OTHER ILLUMINA SERVICES ===
{| class="wikitable" border=1
! ILLUMINA SUPPORTING SERVICES
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
| Quality Control Only -only one QC is included per sequencing lane.
|align="right"| $20
|align="right"| $26
|align="right"| $30
|align="center"| per sample
| Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling.
|align="right"| $10
|align="right"| $13
|align="right"| $15
|align="center"| per sample
| Includes Fluorometric quantification and Frag.Analyzer
|-
|qPCR only (Illumina)
|align="right"| $15
|align="right"| $16.5
|align="right"| $22.5
|align="center"| per sample
| Includes qPCR values from standard QC only
|-
|qPCR only - full plate(Illumina)
|align="right"| $300
|align="right"| $330
|align="right"| $450
|align="center"| per sample
| excludes column 1 (used for controls)
|}

=== LONG READ SEQUENCING ===

{| class="wikitable" border=1
! SEQUEL SEQUENCING
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes0
|-
| 20 hour v3 Chemistry
|align="right"| $1,470
|align="right"| $1,617
|align="right"| $2,205
|align="center"| per SMRTCell
|align="center"| Includes polymerase binding and movie.

|-
! PROMETHION SEQUENCING
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
| 72 hour Flowcell
|align="right"| $1,500
|align="right"| $1,650
|align="right"| $2,100
|align="center"|per Flowcell
||Fastq stored for 1y. Trace data stored only 30d.
|}

== LIBRARY GENERATION ==
=== ILLUMINA LIBRARY - DNA ===

{| class="wikitable" border=1
!width=160| ILLUMINA Sample Preparation
!width=60| Type
!width=60| Batch Size
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
! colspan="4" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
! colspan="3" style="color: blue;" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
|rowspan=3 |LIGATION BASED NEB UltraII
|align="center"|STD
|align="center"| per sample
|align="right"| $105
|align="right"| $115
|align="right"| $157
| Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|- style="color: blue;"
|align="center" rowspan=2|HTL
|align="center"| 24
|align="right"| $790
|align="right"| $869
|align="right"| $1185
|rowspan=2 | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|- style="color: blue;"
|align="center"| 96
|align="right"| $1575
|align="right"| $1732
|align="right"| $2362
|-

|Tagmentation NEXTERA-XT
|align="center" rowspan=2|STD
|align="center" rowspan=2|per Sample
|align="right"| $105
|align="right"| $115
|align="right"| $157
| rowspan="2" | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
|Tagmentation NEXTERA-FLEX
|align="right"| $157
|align="right"| $173
|align="right"| $236

|- style="color: blue;"
|rowspan=2 | NEXTERA-XT OR FLEX
|rowspan=2 align="center"| HTL
|align="center"| ''24''
|align="right"| $790
|align="right"| $869
|align="right"| $1185
|rowspan=2 | Includes tagmentation, indexing, enrichment, and quality control spotcheck(SYBR and FA).
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $1575
|align="right" style="color: blue;" | $1732
|align="right" style="color: blue;" | $2362

|-style="color: blue;"
|rowspan="2" | 16S Amplicon
|align="center" rowspan=2 | HTL
|align="center"|48
|align="right" | $1,890
|align="right" | $2,080
|align="right" | $2,835
|rowspan="2" | Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-style="color: blue;"
|align="center"|add'l 48
|align="right" | $630
|align="right" | $693
|align="right" | $945

|-style="color: blue;"
|rowspan="2" | Other Amplicon
|rowspan=2 align="center"| HTL
|align="center" |48
|align="right" | $1,512
|align="right" | $1,663
|align="right" | $2,268
|rowspan="2" | Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|- style="color: blue;"
|align="center"|add'l 48
|align="right" | $630
|align="right" | $693
|align="right" | $945
|-
! colspan="7" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
|style="color: blue;" colspan=2| High Throughput Setup
|align="center" style="color: blue;" |48 samples
|align="right" style="color: blue;" | $50
|align="right" style="color: blue;" | $55
|align="right" style="color: blue;" | $75
|
|-
|colspan=2|Sample Cleanup
|align="center"|per sample
|align="right"| $5
|align="right"| $5.5
|align="right"| $7.5
|SPRI cleanup of samples to remove impurities.
|-
|style="color: blue;" colspan=2|Quantification
|align="center" style="color: blue;" |per sample
|align="right" style="color: blue;" | $5
|align="right" style="color: blue;" | $5.5
|align="right" style="color: blue;" | $7.5
|Quantification of samples (FA) prior to arraying.
|}

=== ILLUMINA LIBRARY - RNA ===

{| class="wikitable" border=1
!width=160| ILLUMINA Sample Preparation
!width=60| Type
!width=60| Batch Size
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
! colspan="4" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
! colspan="3" style="color: blue;" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included
|-
|rowspan="3"|polyA based isolation Kapa mRNA Hyperprep OR NEB UltraII RNAseq
|align="center"|STD
|align="center"| per sample
|align="right"| $157
|align="right"| $173
|align="right"| $236
|Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
|rowspan=2 align="center" style="color: blue;"| HTL (NEB only)
|align="center" style="color: blue;" | ''24''
|align="right" style="color: blue;" | $1,310
|align="right" style="color: blue;" | $1,444
|align="right" style="color: blue;" | $1,969
|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $2,620
|align="right" style="color: blue;" | $2,888
|align="right" style="color: blue;" | $3,937
|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-

|rowspan="2"| High Throughput 3' Digital Gene Expression (HT3DGE)
|rowspan=2 align="center" style="color: blue;"| HTL
|align="center" style="color: blue;"| ''24''
|align="right" style="color: blue;" | $1050
|align="right" style="color: blue;" | $1155
|align="right" style="color: blue;" | $1575
| rowspan="2" style="color: blue;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $2100
|align="right" style="color: blue;" | $2310
|align="right" style="color: blue;" | $3150
|-

|rowspan="3"|Total RNA sequencing:Mammalian Kapa Hyperprep + RiboErase OR NEB RNAseq +Ribodepletion
|align="center"|STD
|align="center"| per sample
|align="right"| $235
|align="right"| $258.5
|align="right"| $352.5
|Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
|rowspan=2 align="center" style="color: blue;"| HTL (NEB only)
|align="center" style="color: blue;" | ''24''
|align="right" style="color: blue;" | $2100
|align="right" style="color: blue;" | $2310
|align="right" style="color: blue;" | $3150
|rowspan=2|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $4200
|align="right" style="color: blue;" | $4620
|align="right" style="color: blue;" | $6300
|-

|rowspan=3|Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex
|align="center"| '''STD''' v4
|align="center"|per sample
|align="right"| $262
|align="right"| $289
|align="right"| $394
| rowspan="3" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
|rowspan="2" align="center" style="color: blue;"| '''HTL''' v2
|align="center" style="color: blue;" | ''24''
|align="right" style="color: blue;" | $1680
|align="right" style="color: blue;" | $1848
|align="right" style="color: blue;" | $2520
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $2625
|align="right" style="color: blue;" | $2888
|align="right" style="color: blue;" | $3938
|-

|Low Input Mammalian Total Clontech ZapR Kit
|align="center"| STD
|align="center"| per sample
|align="right"| $210
|align="right"| $231
|align="right"| $315
| rowspan="1" | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).

|-
| Small RNA
|align="center"| STD
| align="center"|per sample
| align="right"|$289
| align="right"|$318
| align="right"|$433
| Uses NEB, BIOO or QIAGEN small RNA kit
|-
! colspan="7" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
|colspan=2 style="color: blue;" | High Throughput Setup
|align="center" style="color: blue;" |48 samples
|align="right" style="color: blue;" | $50
|align="right" style="color: blue;" | $55
|align="right" style="color: blue;" | $75
|
|-
|colspan=2|Sample Cleanup
|align="center"|per sample
|align="right"| $5
|align="right"| $5.5
|align="right"| $7.5
|SPRI cleanup of samples to remove impurities.
|-
|colspan=2 style="color: blue;" |Quantification
|align="center" style="color: blue;" |per sample
|align="right" style="color: blue;" | $5
|align="right" style="color: blue;" | $5.5
|align="right" style="color: blue;" | $7.5
|Quantification of samples (FA) prior to arraying.
|-
|}

.

=== LONG READ LIBRARIES ===
{| class="wikitable" border=1
! PacBio Sample Preparation
!width=120| Batch Size
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|rowspan=2|SMRTBell Only eg:PCR products
| Sample 1 || $315 || $346 || $472
|rowspan=2| Includes QC, repair and addition of indexed SMRTBells
|-
| Samples 2-8 || $157 || $173 || $236
|-
|rowspan=2|Genomic Library
| Sample 1 || $472 || $520 || $709
|rowspan=2| Includes Covaris gSPIN fragmentation, Pippin sizing, repair and SMRTbell as above
|-
| Sample 2-8 || $235 || $258 || $352
|-
!Oxford Nanopore Sample Preparation
!width=120| Nanopore Kit
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|Standard Ligation || LSK109 || $150 || $165 || $225
|rowspan=6|Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
|Native Barcoding (ligation indexing) || - || $50 || $55 || $70
|-
|SMARTseq -> Ligation || Cv5+LSK109 || $315 || $346 || $472
|-
|Rapid Prep (tagment - ~10-20kb) || RAD004 || $157 || $173 || $236
|-
|Long Insert Prep || RAD004 (modified, target >100kb) || $262 || $289 || $394
|-
|Direct RNA || RNA002 || $210 || $231 || $315
|}

== SINGLE CELL & SPATIAL SERVICES ==
=== SEQWELL ===
{| class="wikitable" border=1
! 10X USAGE
!width=120| Batch Size
!width=80|KI
!width=80| [[BioMicroCenter:CoreDeps|OTHER CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|colspan=7 align="center"|'''Assisted Service'''
|-
|FULL SERVICE Begin with cells
|per array|| - || - || - || - || Includes all steps, QC of cDNA Begins in KI Nanowell Core
|-
|FROM cDNA on BEADS
|per sample || $350 || $350 || $385 || $525
| Includes exonuclease treatment, amplification, QC of cDNA and library production Must coordinate with BMC staff on submission.
|-
|FROM AMPLIFIED cDNA
|per sample || $100 || $100 || $110 || $150 || Includes QC of cDNA and library production only.
|-
|colspan=7 align="center"|'''Reagents for SeqWell'''
|-
|SeqWell Array
|colspan=6 rowspan="2" | contact shanu@mit.edu for pricing and availability
|-
|SeqWell beads
|}

=== 10X CHROMIUM ===
{| class="wikitable" border=1
! 10X USAGE
!width=120| Batch Size
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|colspan=6 align="center"|'''Assisted Service'''
|-
|colspan=6 |''DIGITAL GENE EXPRESSION''
|-
|rowspan=2 |5'RNA 3' RNA
|setup || $866 || $953 || $1299 || charge per submission
|-
|per sample|| $1455 || $1601 || $2,037 || Includes QC of cDNA and creation of Illumina Library
|-
|rowspan=2 |Amplicon (TCR, BCR, antibody barcocde // per amplicon)
|setup || $367 || $404 || $551 || additional prep beyond DGE
|-
|per sample|| $30 || $33 || $45 || Includes QC of cDNA and creation of Illumina Library
|-
|colspan=6 |''scATACseq''
|-
|rowspan=2 |ATACseq
|setup || $750 || $825|| $1125|| charge per submission
|-
|per sample|| $1560|| $1716|| $2184|| Includes QC and creation of Illumina Library
|-
|colspan=6 |''Multiome (ATAC+3'RNA)''
|-
|rowspan=2 |MULTI
|setup || $1485|| $1633.5|| $2079|| charge per submission
|-
|per sample|| $2575|| $2833|| $3605|| Includes QC of cDNA and creation of Illumina Library
|-
|colspan=6 |''CANCELLATION''
|-
|per session || || $200|| $220|| $280|| <24h notice
|-
|colspan=6 align="center"|'''Walk-up Service'''
|-
|3'/5'RNA Beads and Reagents (v3)|| per samples || $1350 || $1485 || NA ||
|-
|ATACseq Beads and Reagents* || per sample || $1450 || $1595|| NA ||
|-
|multiome Beads and Reagents* || per sample || $2350|| $2585|| NA ||
|-
|Chips (up to 8 sample) || per chip || $350 || $385 || NA || Includes service contract charge.
|-
|Training Limited to 2 people
|per method & per session Reagents not included || $550 || $605 || NA ||
|}
*please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== NAMOCELL ===
{| class="wikitable" border=1
! NAMOCELL
!width=120| Batch Size
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|Usage || Per run || $20 || $22 || $30 || per hour
|-
|Cartridge|| 1 cartridge per sample|| $60 || $66 || $90 ||
|}

=== VISIUM ===
{| class="wikitable" border=1
! SLIDES
!width=120| Batch Size
!width=80| KI Only
!width=80| [[BioMicroCenter:CoreDeps|Other CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|Test Array ||slide|| $1157.75|| $1501.5|| $1510.5|| $2216 || recommended 1x per tissue type
|-
|Sample Slide ||1 slide / 4 tiles|| $5480|| $5614|| $6200|| $7672 || Includes H&E stain images + Library Generation
|}

== OTHER GENOMICS SERVICES ==

 

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===
{| class="wikitable" border=1
! QUALITY CONTROL
!width=100| unit
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=100| MIT
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|Fragment Analyzer: RNA or DNA
|per sample ||$8 || $9 || $12
|
|-
|Fragment Analyzer: Preloaded row
| per row (12) ||$44 ||$48 || $66
| 2ul per sample in each well using plates from BMC
|-
|FemtoPulse: RNA or DNA
|per sample || $12 || $13 || $18
|
|-
|FemtoPulse: Preloaded Row
|per row (12) || $66 || $73 || $99
| 2ul per sample in each well using plates from BMC
|-
|BioAnalyzer: Assisted Service (RNA or DNA)
|per sample || $15 || $18 || $25
|
|-
|BioAnalyzer: Assisted Service (High Sensitivity protein)
|per sample || $40 || $44 || $60
| detection of proteins at < 30ng/ul.
|-
|qPCR only (Illumina quantification test)
|per sample || $20 || $22 || $30
| 4 point serial dilution
|-
|Full plate qPCR (Illumina quantification test)
|per plate || $300 || $330 || $450
| full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
|}

=== [[BioMicroCenter:MicroArrays|MICROARRAYS]] ===

{| class="wikitable" border=1
! MICROARRAYS
!width=100| unit
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=100| MIT
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
| AGILENT SCANNER
|align="center"| per slide
|align="right"| $50
|align="right"| $50
|align="right"| N/A
| Price per glass slide.
|-
|}
Agilent scanning services will end 6/30/2019
 .

== [[BioMicroCenter:RTPCR|REAL TIME PCR]] ==

{| border=1
! OTHER LAB SERVICES
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=100| unit
! SIGN UP
! Notes
|-
| Roche LightCycler 480
|align="right"| $10
|align="right"| N/A
|align="center"| per plate
| [[BioMicroCenter:Forms#Calenders|CALENDAR]] *available for CORE only
| Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| SYBR Green (KAPA/Roche)
|align="right"| $36
|align="right"| N/A
|align="center"| per ml
|
| Available for MIT only
|-
| 96-well PLATES
|align="right"| $6
|align="right"| N/A
|align="center"| per plate
|
| Available for MIT only
|-
|}
.
 

== OTHER LAB SERVICES ==
{| border=1
! OTHER LAB SERVICES
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=100| unit
! SIGN UP
! Notes
|-
|colspan=6 align="center"| '''COVARIS SONICATOR'''
|-
|COVARIS E220
|align="right"|$20
|align="right"|$30
|per hour
|[https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar]
|tubes available for purchase.
|-
| colspan="6" align="center"| '''OLIGO SYNTHESIS'''
|-
|Assisted
|align="right"| $1000 +consumables
|align="right"| $1500 +consumables
|align="center"| per run
|
|includes setup and run of the instrument only.
|-
|Walkup
|align="right"| $500 +consumables
|align="right"| NA
|align="center"| per run
|
|includes =run of the instrument only.
|-
|Training
|align="right"| $2000
|align="right"| N/A
|align="center"|
|
|includes two instrument runs.
|-
| colspan="6" align="center"| '''PIPPIN PREP'''
|-
|Gel Isolation
|align="right"| $50
|align="right"| $75
|align="center"| per gel (5 lanes)
| TBA
|
|-
|Training
|align="right"| $25
|align="right"| $50
|align="center"| per session
| TBA
| Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" align="center"| '''PLATE READER'''
|-
| Varioskan
|align="right"| $18
|align="right"| $27
|align="center"| per hour
| [[BioMicroCenter:Forms#Calenders|CALENDAR]]
| Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" align="center"| '''ROBOTICS'''
|-
| Tecan EVO 150
|align="right"| $50
|align="right"| $55
|align="center"| per 1h block - based on scheduled time.
| [[BioMicroCenter:Forms#Calenders|CALENDAR]]
|
|-
|non-filter tips
|align="right"| $6.00
|align="right"| $6.00
|align="center"| per box
|
|** Please let us know if there is additional plasticware you would like us to stock.
|-
|Filter tips
|align="right"| $8.00
|align="right"| $8.00
|align="center"| per box
|
|
|-
| Training
|align="right"| $50
|align="right"| $55
|align="center"| per session
| Contact [[BioMicroCenter:People|Austin Hendricks]]
|
|-
| colspan="6" align="center"| '''NANODROP'''
|-
| Nanodrop
|align="right"| --
|align="right"| --
|align="center"|
| Walk up service
| * no charge
|-
|}
 

== COMPUTATIONAL SERVICES ==
{| class="wikitable" border=1
! COMPUTATIONAL SERVICES
!width=200| unit
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=100| Other MIT / non-MIT
! Notes
|-
| Informatics Support
| align="right"| per hour
| align="right"| $85
| align="right"| $115 / $160
| Very limited support for non-MIT users
|-
| Informatics Share
| align="right"| per 0.1FTE
| align="right"| $1,200
| align="right"| N/A
| MIT only. Priority access to informatics.
|-
|Ingenuity Pathway Analysis
|align="right"|per dataset
|align="right" | $100
|align="right" | $110/$150
| Recovery of licence cost.
|-
|Active Data storage
|align="right"| per TB per year
|align="right"| $125
|align="right"| $300 / N/A
|
|-
| Archival Storage (read only. Unmutable)
|align="right"| per TB per year
|align="right"| $75
|align="right"| $150 / N/A
|
|-
| Tape Archive / Recovery
|align="right"| per tape
|align="right"| $60
|align="right"| $70
|
|}

BioMicroCenter:PricingFY2022 - Revision history

imported>Stuart S. Levine: /* LONG READ SEQUENCING */