imported>Stuart S. Levine: /* REAL TIME PCR */

2011-06-29T19:11:27Z

REAL TIME PCR

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{{BioMicroCenter}}

== PREVIEW OF PRICING FOR FY2012 ==
'''Pricing is effective beginning on July 1, 2011.'''

== [[BioMicroCenter:Sequencing|ILLUMINA SEQUENCING]] ==

=== SEQUENCING ===
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the Genome Analyzer.

{| border=1 align="left"
! ILLUMINA SEQUENCING
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]/MIT
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=100| unit
! Notes
|-
| colspan="5" align=center | '''GAII / GAIIx''' sequencing (minimum 10m reads/lane. Average ~25-30m reads/lane)
|-
| Single End (36nt)
|align="right"| $810
|align="right"| $1,215
|align="center"| per lane
| Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of Firecrest files for 2 yrs.
|-
| Paired End (36+36nt)
|align="right"| $1,450
|align="right"| $2,175
|align="center"| per lane
|
|-
| Add'l 36nt
|align="right"| $300
|align="right"| $450
|align="center"| per lane
|
|-
| colspan="5" align=center | '''HiSEQ 2000''' sequencing (minimum 25m reads/lane. Average ~75-100m reads/lane)
|-
| Single End (40nt)
|align="right"| $1,170
|align="right"| $1,755
|align="center"| per lane
| Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of Firecrest files for 2 yrs.
|-
| Paired End (40+40nt)
|align="right"| $1,850
|align="right"| $2,775
|align="center"| per lane
|
|-
| Add'l 40nt
|align="right"| $370
|align="right"| $555
|align="center"| per lane
|
|-
| colspan="5" align=center | '''Additional Services'''
|-
| Sample Multiplexing
|align="right"| $50
|align="right"| $75
|align="center"| per 10 samples
| Combined over the whole order. Only applies to samples combined by BMC technicians.
|-
| Add'l Repriming
|align="right"| $200
|align="right"| $300
|align="center"| per flow cell
| Uses 6nt. Cost is divided by number of lanes requiring repriming. Repriming is required for standard Illumina multiplexing.
|-
| Quality Control Only
|align="right"| $40
|align="right"| $60
|align="center"| per sample
|
|}
 

 
Prices effective 7.1.2011 
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the Genome Analyzer. The BioMicro Center reserves the right to reject samples for sample preparations.

=== SAMPLE PREPARATION ===

{| border=1 align="left"
! ILLUMINA Sample Preparation
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]/MIT
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=100| unit
! Notes
|-
| Full Service Sheared DNA
|align="right"| $170
|align="right"| $255
|align="center"| per sample
| Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
|-
| ''SPRI-TE use only''
|align="right"| $110
|align="right"| N/A
|align="center"| per sample
| User must provide all primers. Samples will be returned for amplification.
|-
| Full Service Intact Genomic DNA
|align="right"| $200
|align="right"| $300
|align="center"| per sample
| Preformed using NEXTERA system. Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
|-
| Full Service RNA-seq
|align="right"| $350
|align="right"| $525
|align="center"| per sample
| Preformed using TruSeq AND SPRIworks system. Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
|}
 
 
 
.
 
 
 
 
 
== [[BioMicroCenter:MicroArrays|MICROARRAYS]] ==
Microarray processing prices '''do not''' include the cost of the array. In order to be sure we have arrays on hand, we encourage users to contact us several days in advance of submitting samples.

{| border=1
! MICROARRAYS
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=100| MIT
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=100| unit
! Notes
|-
| colspan="6" | '''AFFYMETRIX PROCESSING'''
|-
| Eukaryote (3' or Exon: >20ng)
|align="right"| $265
|align="right"| $350
|align="right"| $525
|align="center"| per sample
| Using NuGEN sample labeling kit.
|-
| Eukaryote (<20ng)
|align="right"| $375
|align="right"| $470
|align="right"| $700
|align="center"| per sample
| Using NuGEN sample labeling kit.
|-
| Prokaryote
|align="right"| $360
|align="right"| $450
|align="right"| $675
|align="center"| per sample
| Using Ambion sample labeling kit.
|-
| Fluidics Station and Scan
|align="right"| $130
|align="right"| $165
|align="right"| N/A
|align="center"| per array
| Users must be trained and coordinate with Manlin.
|-
| colspan="6" | '''AGILENT PROCESSING'''
|-
| Expression - ONE COLOR - Eukaryote
|align="right"| $175
|align="right"| $235
|align="right"| $350
|align="center"| per array
|
|-
| Expression - TWO COLOR - Eukaryote
|align="right"| $250
|align="right"| $300
|align="right"| $450
|align="center"| per array
| Includes both Cy5 and Cy3
|-
| miRNA - ONE COLOR
|align="right"| $230
|align="right"| $300
|align="right"| $450
|align="center"| per array
|
|-
| Prokaryote - ONE COLOR
|align="right"| $230
|align="right"| $300
|align="right"| $450
|align="center"| per array
|
|-
| Scanning Only
|align="right"| $50
|align="right"| $50
|align="right"| N/A
|align="center"| per slide
| Price per glass slide.
|-
|}
prices effective 7.1.2011
 

'''Common Affymetrix Arrays* (some may be in stock):'''

$175 - Prokaryote/Small Eukaryote E.coli Genome
$250 - Eukaryote 3' (low density) Human U133A 2.0 / Mouse 430A 2.0
$400 - Eukaryote Exon or 3' (high density) Human U133+ 2.0 / Mouse 430 2.0 / Human or Mouse Exon 1.0 ST
Full Price List: [[Media:Affy_Arrays_081218.pdf| Affymetrix Prices]]

'''Common Agilent Expression Arrays*^:'''

$807.20 - 8x15k designs (cost per tile, must use all 8 at once) E.coli / S.cerevisiae / Custom
$170 - 4x44k designs (cost per tile, must use all 4 at once) Human / Mouse / C.elegans / Custom

* available at cost to academic labs only. shipping fees may be added. Non-academic labs should order their own arrays and have them shipped to the BioMicro Center.

^ Discount pricing available through the BioMicro Center.

== [[BioMicroCenter:2100BioAnalyzer|AGILENT BIOANALYZER]] ==
{| border=1
! AGILENT BIOANALYZER
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB]] / MIT
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=100| unit
! Notes
|-
| All DNA and RNA samples
|align="right"| $15
|align="right"| $25
|align="center"| per sample
|
|-
| High Sensitivity protein
|align="right"| $40
|align="right"| $60
|align="center"| per sample
| detection of proteins at < 30ng/ul.
|-
| Flow Cytometry
|align="right"| by request
|align="right"| NA
|align="center"| per sample
|
|-
|}
prices effective 7.1.2011
 .

== [[BioMicroCenter:RTPCR|REAL TIME PCR]] ==

{| border=1
! OTHER LAB SERVICES
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=100| unit
! SIGN UP
! Notes
|-
| colspan="6" align="center"| '''FLUIDIGM'''
|-
| Fluidigm BioMark
|align="right"| $150
|align="right"| $225
|align="center"| per use
| [[BioMicroCenter:Forms#Calenders|CALENDER]]
| Contact [[BioMicroCenter:People|Ryan]]
|-
| 48.48 Dynamic Array Chip
|align="right"| $260
|align="right"| N/A
|align="center"| per cassette
| Cassettes are available in the BMC.
| Contact [[BioMicroCenter:People|Ryan]]
|-
| 96.96 Dynamic Array Chip
|align="right"| $760
|align="right"| N/A
|align="center"| per cassette
| Cassettes are available in the BMC.
| Contact [[BioMicroCenter:People|Ryan]]
|-
| colspan="6" align="center"| '''RT-PCR'''
|-
| Roche LightCycler 480
|align="right"| $10
|align="right"| NA
|align="center"| per plate
| [[BioMicroCenter:Forms#Calenders|CALENDER]] *available for CORE only
| Contact [[BioMicroCenter:People|Michael]] for training
|-
| MJ Opticon II
|align="right"| $10
|align="right"| $15
|align="center"| per plate
| [[BioMicroCenter:Forms#Calenders|CALENDER]]
| Contact [[BioMicroCenter:People|Manlin]] for training
|-
| SYBR Green (KAPA/Roche)
|align="right"| $34
|align="right"| NA
|align="center"| per ml
|
| Available for MIT only
|-
| 96-well RTPCR PLATE
|align="right"| $6
|align="right"| NA
|align="center"| per plate
|
| Available for MIT only
|-
|}
prices effective 7.1.2011
.
 

== OTHER LAB SERVICES ==

{| border=1
! OTHER LAB SERVICES
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=100| unit
! SIGN UP
! Notes
|-
| colspan="6" align="center"| '''PLATE READER'''
|-
| Varioskan
|align="right"| $10
|align="right"| $15
|align="center"| per plate
| [[BioMicroCenter:Forms#Calenders|CALENDER]]
| Quick scans (those requiring less then 30 minutes) are free of charge but may not be scheduled on the calender. Users who have large experiments have priority. Contact [[BioMicroCenter:People|Kevin]] for training
|-
| colspan="6" align="center"| '''ROBOTICS'''
|-
| Tecan EVO 150
|align="right"| $50
|align="right"| $150
|align="center"| per 2h block - based on scheduled time.
| [[BioMicroCenter:Forms#Calenders|CALENDER]]
|
|-
|non-filter tips
|align="right"| $5.00
|align="right"| $5.00
|align="center"| per box
|
|** Please let us know if there is additional plasticware you would like us to stock.
|-
|Filter tips
|align="right"| $8.00
|align="right"| $8.00
|align="center"| per box
|
|
|-
| Training
|align="right"| $100
|align="right"| $150
|align="center"| per session
| Contact [[BioMicroCenter:People|Barbara]]
|
|-
| colspan="6" align="center"| '''NANODROP'''
|-
| Nanodrop
|align="right"| --
|align="right"| --
|align="center"|
| Walk up service
| * no charge
|-
|}
prices effective 7.1.2011
 

== COMPUTATIONAL SERVICES ==
{| border=1
! COMPUTATIONAL SERVICES
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=100| Other MIT / [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=100| unit
! Notes
|-
| Data storage (1 year)
|align="right"| $500
|align="right"| N/A
|align="center"| per TB
| (Limited space available)
|-
| Spotfire License (1 year)
|align="right"| ''Contact Stephen Goldman''
|align="right"| N/A
|align="center"| per user
| (Limited seats available)
|-
|}

BioMicroCenter:Pricing2012 - Revision history

imported>Stuart S. Levine: /* REAL TIME PCR */