imported>Stuart S. Levine at 17:27, 31 May 2012

2012-05-31T17:27:59Z

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{{BioMicroCenter}}

== Evaluation of the [[BioMicroCenter:PippinPrep|BluePippin Prep:]] ==
{| border="0"
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|'''Isolation of individual bands'''
|Here, the pippin prep has been used to isolate individual bands (lane 1-5) of a 50bp ladder (lane L). This isolation can be preprogrammed or based on peak position. Band isolation is useful for cloning and purification of PCR products.
|[[Image:BMC_pippinTest1b.png|thumb|200px]]
|[[Image:BMC_pippinTest1a.png|thumb|200px]]
|-
|'''Creating tight size distributions'''
| Here the Pippin prep was used to isolate tight bands from a broad distribution. Band sizes of +/-25bp can be isolated from 1.5% agarose cassettes. Tight size selection is useful in RNAseq and small RNAseq.
|[[Image:BMC_pippinTest2c.png|thumb|200px]]
|[[Image:BMC_pippinTest2b.png|thumb|200px]]
|-
|'''How tight can the Blue Pippin go'''
| Effect of band window on yield. To evaluate the importance of the window size on yield, we tested different sized windows on a standard Illumina library. We do notice significant decreases in yield when the window is smaller than 80bp without a comparable increase in resolution.
|[[Image:BMC_pippinTest3.png|thumb|200px]]
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|}

BioMicroCenter:PippinPrep Testing - Revision history

imported>Stuart S. Levine at 17:27, 31 May 2012