http://bmcwiki.mit.edu/index.php?action=history&feed=atom&title=BioMicroCenter%3APartialFlowcellsBioMicroCenter:PartialFlowcells - Revision history2026-04-29T03:55:27ZRevision history for this page on the wikiMediaWiki 1.43.1http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:PartialFlowcells&diff=112&oldid=previmported>Stuart S. Levine at 20:42, 2 June 20122012-06-02T20:42:01Z<p></p>
<p><b>New page</b></p><div>{{BioMicroCenter}}<br />
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In order to maintain the utility of our GAIIs, the BioMicro Center has developed methods to run the GA in a lane-wise manner. These methods are not supported by Illumina and we are learning by trial and error. The partial flowcell effort was lead by [[BioMicroCenter:People|Michael Gravina]] in the BioMicro Center.<br />
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== Testing ==<br />
{|<br />
|-align="top"<br />
|'''Testing lanewise flow''' <br />
|Food coloring was loaded in the GAII using one of the standard 50ml conicals and flowed through a used flowcell either 1 lane at a time or 4 lanes at a time.<br />
|[[Image:BMC_partialFlowcell.png|thumb|200px|Food coloring in GAII flowcells]]<br />
|-align="top"<br />
|'''Testing reclustering of the flowcell'''<br />
|A single flowcell was subjected to 5 cycles of clustering and sequencing over ~2 weeks and evaluated for various quality parameters. PhiX was used as the test sample. Sequencing reagents were replaced for the 4th sequencing cycle.<br />
|[[Image:BMC_partialClusterCount.png|thumb|200px|Count of clusters across clustering cycles]]<BR><br />
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|[[Image:BMC_cycle10.png|thumb|200px|Intensity of bases across clustering cycles]]<BR><br />
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|[[Image:BMC_partialBaseBalance.png|thumb|200px|Base balance at base 10 across clustering cycles]]<BR><br />
|}<br />
== Scripts ==<br />
We will soon be publishing the scripts we use on the GAII to run partial flowcells. Keep an eye on this space.</div>imported>Stuart S. Levine