Hilo1: /* Library Preparation Services */

2025-05-27T17:44:47Z

Library Preparation Services

← Older revision		Revision as of 17:44, 27 May 2025
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	{{BioMicroCenter}}		{{BioMicroCenter}}
	=Library Preparation Services=		=Library Preparation Services=
	[[Image:BMC_samplePrep_pipes.png\|thumb\|right\|~~400px~~\|Sample Preparation available in BioMicro]]		[[Image:BMC_samplePrep_pipes.png\|thumb\|right\|200px\|Sample Preparation available in BioMicro]]
	Generating high-quality data on the Illumina sequencing platform requires high-quality libraries. The BMC currently offers library preparation services for a variety of starting materials. Prior to sequencing, all samples must pass the BioMicro Center’s Sequencing Quality Control process, which verifies selection of inserts of a desired size and correct ligation of Illumina adapters.		Generating high-quality data on the Illumina sequencing platform requires high-quality libraries. The BMC currently offers library preparation services for a variety of starting materials. Prior to sequencing, all samples must pass the BioMicro Center’s Sequencing Quality Control process, which verifies selection of inserts of a desired size and correct ligation of Illumina adapters.

imported>Sam.mildrum: /* Illumina Library Prep Services */

2020-07-01T20:15:52Z

Illumina Library Prep Services

New page

{{BioMicroCenter}}
=Library Preparation Services=
[[Image:BMC_samplePrep_pipes.png|thumb|right|400px|Sample Preparation available in BioMicro]]
Generating high-quality data on the Illumina sequencing platform requires high-quality libraries. The BMC currently offers library preparation services for a variety of starting materials. Prior to sequencing, all samples must pass the BioMicro Center’s Sequencing Quality Control process, which verifies selection of inserts of a desired size and correct ligation of Illumina adapters.

==Illumina Library Prep Services==
Please follow the links in the table below for more information about our library preparation offerings.
{| class="wikitable" border=1
|-
! style="text-align: center;" | METHOD TYPE
! style="text-align: center;" | DNA
! style="text-align: center;" | RNA
|-
| style="text-align: center; height: 2em; font-size: 16px;" | STANDARD
| style="text-align: left; vertical-align: top; height: 2em;" | '''Ligation-mediated PCR Methods'''
* [[BioMicroCenter:DNA_LIB#NEB_Ultra_II | NEB Ultra II]]
'''Tagmentation Methods'''
* [[BioMicroCenter:DNA_LIB#Nextera_Flex | Nextera Flex]]
* [[BioMicroCenter:DNA_LIB#Nextera_XT | Nextera XT]]
| style="text-align: left; height: 2em;" | '''Standard Input'''
* [[BioMicroCenter:RNA_LIB | NEB Ultra II Directional RNA]] with:    [[BioMicroCenter:RNA_LIB#NEB_Ultra_II_Directional_RNA_with_Poly(A)_Selection | Poly(A) mRNA Isolation]]    [[BioMicroCenter:RNA_LIB#NEB_Ultra_II_Directional_RNA_with_rRNA_Depletion_.28H.2FM.2FR.29 | rRNA Depletion (H/M/R)]]    rRNA Depletion (Bacterial)
'''Low Input'''
* [[BioMicroCenter:RNA_LIB#Clontech_SMARTseq_Low-Input | Clontech SMART-Seq v4]]    - Poly(A) based selection
* [[BioMicroCenter:RNA_LIB#Clontech_SMARTer_Stranded_Total_RNA-Seq_Kit_-_Pico_Input_Mammalian_--_aka_Clontech_ZapR | Clontech ZapR]]    - rRNA depletion based
''Small RNA upon request''
|-
| style="text-align: center; height: 2em;" | HIGH THROUGHPUT
| style="text-align: top-left; height: 2em;" | '''Ligation-mediated PCR Methods'''
* NEB Ultra II
'''Tagmentation Methods'''
* Nextera Flex
* Nextera XT
'''Amplicon Methods'''
* 16S Metagenomic
* Custom Amplicon
| style="text-align: left; height: 2em;" | '''Standard Input'''
* NEB Ultra II Directional RNA with:    Poly(A) mRNA Isolation    rRNA Depletion (H/M/R)
* 3' Digital Gene Expression (3'DGE)
'''Low Input'''
* Clontech SMART-Seq v2    - Poly(A) based selection
* Clontech ZapR    - rRNA depletion based selection
|}

== OTHER SERVICES ==
=== SIZE SELECTION ===
The BMC offers [[BioMicroCenter:PippinPrep|PippinPrep Size Selection]] post library construction if custom insert sizes are needed.
=== QUALITY CONTROL ===
All NGS libraries are quality controlled prior to use. The BioMicro Center offers two 'flavors' of quality control for Illumina libraries. 
Standard quality control includes analysis on the [[BioMicroCenter:QC#AATI_FRAGMENT_ANALYZER|Fragment Analyzer]] and quantification by four point [[BioMicroCenter:RTPCR|qPCR]] quantification for each sample. This confirms both the sizing as well as the presence of Illumina adapters on the libraries. 
Rapid Quality Control is less expensive and quicker. It includes analysis on the [[BioMicroCenter:QC#AATI_FRAGMENT_ANALYZER|Fragment Analyzer]] and quantification by PicoGreen on the [[BioMicroCenter:Varioskan|Varioskan]] for each sample. Final pools are quantified by [[BioMicroCenter:RTPCR|qPCR]] before loading. While this method is significantly faster and less expensive, we cannot guarantee pool balance with it.

BioMicroCenter:PREP TEST - Revision history

Hilo1: /* Library Preparation Services */

imported>Sam.mildrum: /* Illumina Library Prep Services */