http://bmcwiki.mit.edu/index.php?action=history&feed=atom&title=BioMicroCenter%3ANanoDrop_ND-1000 2026-04-15T00:35:11Z Revision history for this page on the wiki MediaWiki 1.43.1 http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:NanoDrop_ND-1000&diff=82&oldid=prev 2009-01-20T18:03:08Z

<p><span class="autocomment">Applications</span></p> <p><b>New page</b></p><div>{{BioMicroCenter}} <br /> <br /> <br /> [[Image:Nd-1000.jpg|right|200px]]<br /> == NanoDrop ND-1000 == <br /> <br /> The NanoDrop ND-1000 is a [http://en.wikipedia.org/wiki/Full-spectrum full-spectrum] (220 - 750nm) spectrophotometer that measures 1ul samples without the use of cuvettes or the need for dilution.<br /> <br /> == Applications == <br /> <br /> * Concentration and purity measurements of nucleic acids (DNA, RNA, oligos, PCR product, and hybridization probes). With a detection limit of about 2ng/ul (dsDNA) but has shown to be significantly variable at lower concentrations. <br /> <br /> * Measures nucleic acid probe and dye concentrations to help determine microarray labeling efficiency (Cy3, Cy5, and Alexaflour dyes)<br /> <br /> * Concentration and purity measurements of labeled and unlabeled proteins (Bradford, BCA, and Lowry assays). With a detection limit of about 0.1mg/ml(BSA). <br /> <br /> * Can perform [http://en.wikipedia.org/wiki/Full-spectrum full-spectrum] UV/Vis spectrophotometry (220nm - 750nm)<br /> <br /> * Can determine cell density of microbial cultures in suspension<br /> <br /> == Location == <br /> The NanoDrop ND-1000 is located at the BMC, building 68 room 316 and is FREE for [[BioMicroCenter:CoreDeps|core department]] use</div>

imported>Allison Perrotta