http://bmcwiki.mit.edu/index.php?action=history&feed=atom&title=BioMicroCenter%3ACoverageCalculationsBioMicroCenter:CoverageCalculations - Revision history2026-05-15T05:30:16ZRevision history for this page on the wikiMediaWiki 1.43.1http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:CoverageCalculations&diff=42&oldid=previmported>Stuart S. Levine: /* Determining ideal read length and depth of coverage */2013-01-11T02:21:39Z<p><span class="autocomment">Determining ideal read length and depth of coverage</span></p>
<p><b>New page</b></p><div>{{BioMicroCenter}}<br />
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==Determining ideal read length and depth of coverage==<br />
The BioMicro Center offers a wide variety of read lengths, both in single-end and paired-end formats. Often, it is useful to calculate the expected average coverage. <BR><BR><br />
<br />
Coverage for genomic samples can be calculated as:<br />
<br />
no.reads(1/2) * readlength * no.cluster<br />
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genome size <br />
<br />
For ChIP samples, the following modified formula can be used:<br />
<br />
no.reads * readlength * no.cluster<br />
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no.sites * site.length / % reads in sites<br />
<br />
Some standard genome sizes:<br />
</div>imported>Stuart S. Levine