BioMicroCenter:Covaris - Revision history

Gibcus: /* R230 */

2025-11-14T17:01:03Z

R230

← Older revision		Revision as of 17:01, 14 November 2025
Line 26:		Line 26:

	The Covaris R230 is a critical component in High-throughput libraries produced using ribosomal depletion methods.<BR><BR>		The Covaris R230 is a critical component in High-throughput libraries produced using ribosomal depletion methods.<BR><BR>
			<BR><BR>
	The Covaris R230 was funded by an NIH grant and R24OD035444 should be included in any publications using the instrument.		The Covaris R230 was funded by an NIH grant and R24OD035444 should be included in any publications using the instrument.
	\|}		\|}

Gibcus: /* R230 */

2025-11-14T17:00:38Z

R230

← Older revision		Revision as of 17:00, 14 November 2025
Line 21:		Line 21:

	== R230 ==		== R230 ==
	[[IMAGE:R230pic.jpeg\|right\|250px\| Covaris R230]]		[[IMAGE:R230pic.jpeg\|thumb\|right\|250px\| Covaris R230]]
	~~<br><br>~~
	The Covaris R230 is a high throughput sonicator fitted for 96 AFA-TUBE® TPX Plates. Both RNA and DNA have been sheared with similar programs to the ones found on the E220 Evolution at maximum input 5 µg 20-50 uL per well in the sonication by column mode. With this column sonication mode, an entire 96 well plate can be sonicated 10x faster in the R230 versus the E220. <BR><BR>		The Covaris R230 is a high throughput sonicator fitted for 96 AFA-TUBE® TPX Plates. Both RNA and DNA have been sheared with similar programs to the ones found on the E220 Evolution at maximum input 5 µg 20-50 uL per well in the sonication by column mode. With this column sonication mode, an entire 96 well plate can be sonicated 10x faster in the R230 versus the E220. <BR><BR>

Gibcus: /* R230 */

2025-11-14T16:56:57Z

R230

← Older revision		Revision as of 16:56, 14 November 2025
Line 23:		Line 23:
	[[IMAGE:R230pic.jpeg\|right\|250px\| Covaris R230]]		[[IMAGE:R230pic.jpeg\|right\|250px\| Covaris R230]]
	<br><br>		<br><br>
	The Covaris R230 is a high throughput sonicator fitted for 96 AFA-TUBE® TPX Plates. Both RNA and DNA have been sheared with similar programs to the ones found on the E220 Evolution at maximum input 5 ug 20-50 uL per well in the sonication by column mode. With this column sonication mode, an entire 96 well plate can be sonicated 10x faster in the R230 versus the E220. <BR><BR>		The Covaris R230 is a high throughput sonicator fitted for 96 AFA-TUBE® TPX Plates. Both RNA and DNA have been sheared with similar programs to the ones found on the E220 Evolution at maximum input 5 µg 20-50 uL per well in the sonication by column mode. With this column sonication mode, an entire 96 well plate can be sonicated 10x faster in the R230 versus the E220. <BR><BR>

	The Covaris R230 is a critical component in High-throughput libraries produced using ribosomal depletion methods.<BR><BR>		The Covaris R230 is a critical component in High-throughput libraries produced using ribosomal depletion methods.<BR><BR>

Gibcus: /* E220 Evolution */

2025-11-14T16:55:32Z

E220 Evolution

← Older revision		Revision as of 16:55, 14 November 2025
Line 8:		Line 8:
	[[Image:E220.jpg‎\|thumb\|right\|250px\| Covaris E220 Evo]]		[[Image:E220.jpg‎\|thumb\|right\|250px\| Covaris E220 Evo]]

	The [https://www.covaris.com/e220evolution-focused-ultrasonicator-500429/ Covaris E220 evolution sonicator] is a multitube system that processes single samples at a time. The E220 evolution is capable of processing a wide range of sample types and volumes and may be programed to process from 1 to 96 samples in a single batch. The E220 is most appropriate < 5µg clean DNA in 130µl (microTUBE-130) or 20-120 mg tissue mass in 1.0 ml (milliTUBE-1). Using adaptive focused acoustic energy, the E220 utilizing a spherical acoustic transducer, and delivers controlled energy precisely and accurately to sample volumes from 25 μl to 10 ml. The ~~E220 is the~~ most powerful Covaris instrument ~~allowing~~ not only ~~shearing of~~ DNA and RNA, but it also chromatin. Furthermore, it can homogenize tissue for cell lysis and drug delivery and formulation.		The [https://www.covaris.com/e220evolution-focused-ultrasonicator-500429/ Covaris E220 evolution sonicator] is a multitube system that processes single samples at a time. The E220 evolution is capable of processing a wide range of sample types and volumes and may be programed to process from 1 to 96 samples in a single batch. The E220 is most appropriate < 5µg clean DNA in 130µl (microTUBE-130) or 20-120 mg tissue mass in 1.0 ml (milliTUBE-1). Using adaptive focused acoustic energy, the E220 utilizing a spherical acoustic transducer, and delivers controlled energy precisely and accurately to sample volumes from 25 μl to 10 ml. The most powerful Covaris instrument, the E220 can shear not only DNA and RNA, but also chromatin. Furthermore, it can homogenize tissue for cell lysis and drug delivery and formulation.
	<BR><BR>		<BR><BR>
	PROTOCOLS FOR [https://www.covaris.com/protocols?filter_tag=DNA%20Shearing%20Protocols/ DNA]		PROTOCOLS FOR [https://www.covaris.com/protocols?filter_tag=DNA%20Shearing%20Protocols/ DNA]

Gibcus: /* E220 Evolution */

2025-11-14T16:54:28Z

E220 Evolution

← Older revision		Revision as of 16:54, 14 November 2025
Line 8:		Line 8:
	[[Image:E220.jpg‎\|thumb\|right\|250px\| Covaris E220 Evo]]		[[Image:E220.jpg‎\|thumb\|right\|250px\| Covaris E220 Evo]]

	The [https://www.covaris.com/e220evolution-focused-ultrasonicator-500429/ Covaris E220 evolution sonicator] is a multitube system that processes single samples at a time. The E220 evolution is capable of processing a wide range of sample types and volumes and may be programed to process from 1 to 96 samples in a single batch. The E220 is most appropriate <~~5ug~~ clean DNA in ~~130ul~~ (microTUBE-130) or 20-120 mg tissue mass in 1.0 ml (milliTUBE-1). Using adaptive focused acoustic energy, the E220 utilizing a spherical acoustic transducer, and delivers controlled energy precisely and accurately to sample volumes from 25 μl to 10 ml. The ~~main applications are~~ shearing of DNA, RNA, ~~and~~ chromatin ~~but~~ it can ~~also perform~~ tissue ~~homogenization~~ for cell lysis and drug delivery and formulation.		The [https://www.covaris.com/e220evolution-focused-ultrasonicator-500429/ Covaris E220 evolution sonicator] is a multitube system that processes single samples at a time. The E220 evolution is capable of processing a wide range of sample types and volumes and may be programed to process from 1 to 96 samples in a single batch. The E220 is most appropriate < 5µg clean DNA in 130µl (microTUBE-130) or 20-120 mg tissue mass in 1.0 ml (milliTUBE-1). Using adaptive focused acoustic energy, the E220 utilizing a spherical acoustic transducer, and delivers controlled energy precisely and accurately to sample volumes from 25 μl to 10 ml. The E220 is the most powerful Covaris instrument allowing not only shearing of DNA and RNA, but it also chromatin. Furthermore, it can homogenize tissue for cell lysis and drug delivery and formulation.
	<BR><BR>		<BR><BR>
	PROTOCOLS FOR [https://www.covaris.com/protocols?filter_tag=DNA%20Shearing%20Protocols/ DNA]		PROTOCOLS FOR [https://www.covaris.com/protocols?filter_tag=DNA%20Shearing%20Protocols/ DNA]

Gibcus: /* E220 Evolution */

2025-11-14T16:05:19Z

E220 Evolution

← Older revision		Revision as of 16:05, 14 November 2025
Line 8:		Line 8:
	[[Image:E220.jpg‎\|thumb\|right\|250px\| Covaris E220 Evo]]		[[Image:E220.jpg‎\|thumb\|right\|250px\| Covaris E220 Evo]]

	The [https://www.covaris.com/e220evolution-focused-ultrasonicator-500429/ Covaris E220 evolution sonicator] is a multitube system that processes single samples at a time. The ~~E220evolution~~ is capable of processing a wide range of sample types and volumes and may be programed to process from 1 to 96 samples in a single batch. The E220 is most appropriate <5ug clean DNA in 130ul (microTUBE-130) or 20-120 mg tissue mass in 1.0 ml (milliTUBE-1). Using adaptive focused acoustic energy, the E220 utilizing a spherical acoustic transducer, and delivers controlled energy precisely and accurately to sample volumes from 25 μl to 10 ml. The main applications are shearing of DNA, RNA, and chromatin but it can also perform tissue homogenization for cell lysis and drug delivery and formulation.		The [https://www.covaris.com/e220evolution-focused-ultrasonicator-500429/ Covaris E220 evolution sonicator] is a multitube system that processes single samples at a time. The E220 evolution is capable of processing a wide range of sample types and volumes and may be programed to process from 1 to 96 samples in a single batch. The E220 is most appropriate <5ug clean DNA in 130ul (microTUBE-130) or 20-120 mg tissue mass in 1.0 ml (milliTUBE-1). Using adaptive focused acoustic energy, the E220 utilizing a spherical acoustic transducer, and delivers controlled energy precisely and accurately to sample volumes from 25 μl to 10 ml. The main applications are shearing of DNA, RNA, and chromatin but it can also perform tissue homogenization for cell lysis and drug delivery and formulation.
	<BR><BR>		<BR><BR>
	PROTOCOLS FOR [https://www.covaris.com/protocols?filter_tag=DNA%20Shearing%20Protocols/ DNA]		PROTOCOLS FOR [https://www.covaris.com/protocols?filter_tag=DNA%20Shearing%20Protocols/ DNA]
Line 19:		Line 19:

	\|style=width:49%\|		\|style=width:49%\|

	== R230 ==		== R230 ==
	[[IMAGE:R230pic.jpeg\|right\|250px\| Covaris R230]]		[[IMAGE:R230pic.jpeg\|right\|250px\| Covaris R230]]

Slevine: /* R230 */

2025-05-17T23:45:32Z

R230

← Older revision		Revision as of 23:45, 17 May 2025
Line 22:		Line 22:
	[[IMAGE:R230pic.jpeg\|right\|250px\| Covaris R230]]		[[IMAGE:R230pic.jpeg\|right\|250px\| Covaris R230]]
	<br><br>		<br><br>
	The Covaris R230 is a high throughput sonicator fitted for 96 AFA-TUBE® TPX Plates. Both RNA and DNA have been sheared with similar programs to the ones found on the E220 Evolution at maximum input 5 ug 20-50 uL per well in the sonication by column mode. With this column sonication mode, an entire 96 well plate can be sonicated 10x faster in the R230 versus the E220.		The Covaris R230 is a high throughput sonicator fitted for 96 AFA-TUBE® TPX Plates. Both RNA and DNA have been sheared with similar programs to the ones found on the E220 Evolution at maximum input 5 ug 20-50 uL per well in the sonication by column mode. With this column sonication mode, an entire 96 well plate can be sonicated 10x faster in the R230 versus the E220. <BR><BR>

			The Covaris R230 is a critical component in High-throughput libraries produced using ribosomal depletion methods.<BR><BR>

			The Covaris R230 was funded by an NIH grant and R24OD035444 should be included in any publications using the instrument.
	\|}		\|}

Slevine: /* SONICATORS */

2025-05-17T21:46:29Z

SONICATORS

← Older revision		Revision as of 21:46, 17 May 2025
Line 3:		Line 3:
	= SONICATORS =		= SONICATORS =
	{\|		{\|
			\|-style=vertical-align:top
			\|style=width:49%\|
	== E220 Evolution ==		== E220 Evolution ==
			[[Image:E220.jpg‎\|thumb\|right\|250px\| Covaris E220 Evo]]

	The [https://www.covaris.com/e220evolution-focused-ultrasonicator-500429/ Covaris E220 evolution sonicator] is a multitube system ~~developed for high performance sample preparation~~. The E220evolution is capable of processing a wide range of sample types and volumes and may be programed to process from 1 to 96 samples in a single batch. The E220 is most appropriate <5ug clean DNA in 130ul (microTUBE-130) or 20-120 mg tissue mass in 1.0 ml (milliTUBE-1). Using adaptive focused acoustic energy, the E220 utilizing a spherical acoustic transducer, and delivers controlled energy precisely and accurately to sample volumes from 25 μl to 10 ml. The main applications are shearing of DNA, RNA, and chromatin but it can also perform tissue homogenization for cell lysis and drug delivery and formulation.		The [https://www.covaris.com/e220evolution-focused-ultrasonicator-500429/ Covaris E220 evolution sonicator] is a multitube system that processes single samples at a time. The E220evolution is capable of processing a wide range of sample types and volumes and may be programed to process from 1 to 96 samples in a single batch. The E220 is most appropriate <5ug clean DNA in 130ul (microTUBE-130) or 20-120 mg tissue mass in 1.0 ml (milliTUBE-1). Using adaptive focused acoustic energy, the E220 utilizing a spherical acoustic transducer, and delivers controlled energy precisely and accurately to sample volumes from 25 μl to 10 ml. The main applications are shearing of DNA, RNA, and chromatin but it can also perform tissue homogenization for cell lysis and drug delivery and formulation.
	<BR><BR>		<BR><BR>
	[https://www.covaris.com/protocols?filter_tag=DNA%20Shearing%20Protocols/ DNA] or [https://www.covaris.com/protocols?filter_tag=Chromatin%20Shearing%20Protocols/ Chromatin, FFPE] ~~etc., protocols for optimizing the sonication time for next generation sequencing can be found on the company's website: [https://www.covaris.com/ Covaris]~~		PROTOCOLS FOR [https://www.covaris.com/protocols?filter_tag=DNA%20Shearing%20Protocols/ DNA]
	~~<br><br>~~		PROTOCOLS FOR [https://www.covaris.com/protocols?filter_tag=Chromatin%20Shearing%20Protocols/ Chromatin, FFPE] e
	~~The manual can be found here~~: [https://www.covaris.com/e220evolution-focused-ultrasonicator-500429/ E220 evolution].		MANUAL: [https://www.covaris.com/e220evolution-focused-ultrasonicator-500429/ E220 evolution].
	<br>		<br>

	[[BioMicroCenter:Covaris_Maintenance\| E220 Maintenance Instructions]]		[[BioMicroCenter:Covaris_Maintenance\| E220 Maintenance Instructions]]
			\|style=width:2%\|

	~~[[Image~~:~~E220.jpg‎\|thumb\|right\|250px\| Covaris E220 Evolution]]~~		\|style=width:49%\|


	\|

	== R230 ==		== R230 ==
			[[IMAGE:R230pic.jpeg\|right\|250px\| Covaris R230]]
	[[IMAGE:R230pic.jpeg\|right\|~~350px~~\|R230 ~~Focused ultrasonicator 1.0 (not pictured: eBox and WCS 3.0)~~]]


	<br><br>		<br><br>
	The Covaris R230 is fitted for 96 AFA-TUBE® TPX Plates. Both RNA and DNA have been sheared with similar programs to the ones found on the E220 Evolution at maximum input 5 ug 20-50 uL per well in the sonication by column mode. With this column sonication mode, an entire 96 well plate can be sonicated 10x faster in the R230 versus the E220~~. A scanning mode exists but has not been tested yet. There are accessories to utilize both 8-well strips and 384 well plates, but these accessories have not been acquired~~.		The Covaris R230 is a high throughput sonicator fitted for 96 AFA-TUBE® TPX Plates. Both RNA and DNA have been sheared with similar programs to the ones found on the E220 Evolution at maximum input 5 ug 20-50 uL per well in the sonication by column mode. With this column sonication mode, an entire 96 well plate can be sonicated 10x faster in the R230 versus the E220.
	\|}		\|}

Slevine: /* E220 Evolution */

2025-05-17T21:28:47Z

E220 Evolution

@@ Line 5: / Line 5: @@
 == E220 Evolution ==
 The [https://www.covaris.com/e220evolution-focused-ultrasonicator-500429/ Covaris E220 evolution sonicator] is a multitube system developed for high performance sample preparation.  The E220evolution is capable of processing a wide range of sample types and volumes and may be programed to process from 1 to 96 samples in a single batch. The E220 is most appropriate <5ug clean DNA in 130ul (microTUBE-130) or 20-120 mg tissue mass in 1.0 ml (milliTUBE-1). Using adaptive focused acoustic energy, the E220 utilizing a spherical acoustic transducer, and delivers controlled energy precisely and accurately to sample volumes from 25 μl to 10 ml. The main applications are shearing of DNA, RNA, and chromatin but it can also perform tissue homogenization for cell lysis and drug delivery and formulation.
@@ Line 14: / Line 13: @@
 <br>
-[[BioMicroCenter:Covaris_Maintenance| R230 Maintenance Instructions]]
+[[BioMicroCenter:Covaris_Maintenance| E220 Maintenance Instructions]]
 [[Image:E220.jpg‎|thumb|right|250px| Covaris E220 Evolution]]
+|

Slevine: /* E220 Evolution */

2025-05-17T21:26:14Z

E220 Evolution

← Older revision		Revision as of 21:26, 17 May 2025
Line 14:		Line 14:
	<br>		<br>

			[[BioMicroCenter:Covaris_Maintenance\| R230 Maintenance Instructions]]

	[[Image:E220.jpg‎\|thumb\|right\|250px\| Covaris E220 Evolution]]		[[Image:E220.jpg‎\|thumb\|right\|250px\| Covaris E220 Evolution]]
Line 72:		Line 72:

	\|		\|

	== R230 ==		== R230 ==