View source for BioMicroCenter:Protocols

{{BioMicroCenter}}

== BioMicro Center Protocol Testing ==
* [[BioMicroCenter:RTPCR Protocol for Sample QC|RT-PCR Protocol for Sample QC]]
* [[BioMicroCenter:Cluster Visualization Protocol for Flowcell QC|Cluster Visualization Protocol for Flowcell QC]]

== [[BioMicroCenter:Sequencing|ILLUMINA SEQUENCING PROTOCOLS]] ==
To access the official Illumina protocols please got to the [http://illumina.com Illumina website] and create an iCom account. Once an iCom account has been created please feel free to use the links below to download specific protocols.

[[Media: BioMicro_Single_Read_Illumina_Primer_Seq_WorkFlow.xlsx | Illumina Single Read and Multiplexing Sequence Workflow]] <br>

<B><BIG> Cluster Station and Genome Analyzer </BIG></B>
*[https://icom.illumina.com/Download/Summary/6OVjeOd3X0aH0rzu0JGTYQ Cluster Station Operations Guide] 
*[https://icom.illumina.com/Download/Summary/T8b3pGVc_EumaUulIvCw_w Cluster Station User Guide]
*[https://icom.illumina.com/Download/Summary/pR7OF2lK7029XIt0-dkSdQ Genome Analyzer II User Guide]
*[[Media:LaneByLaneConverterV4.zip|Python script for Lane-by-lane GAII recipe conversion (version 4, compatible with SCS2.10 recipes).]]

<B><BIG> ChIP-Seq </BIG></B>
*[https://icom.illumina.com/Download/Summary/Yy2liOS-nEWyTUnT5w9UMA ChIP-Seq Sample Prep Guide]

<B><BIG> mRNA-Seq </BIG></B>
*[https://icom.illumina.com/Download/Summary/rOfzT_z3S0C4de1GFDL7qQ Directional RNA-Seq Library Prep Pre-Release]
*[https://icom.illumina.com/Download/Summary/kUyOp1caIk6Sea-x5DN6hw DSN Normalization Sample Prep Guide]
*[https://icom.illumina.com/Download/Summary/A4zkeV3eLUOO2mzisssR1g mRNA-Seq Sample Prep Guide]

<B><BIG> SmallRNA-Seq </BIG></B>
*[https://icom.illumina.com/Download/Summary/MBkxLGP9Q0u_psYnP0xQCw SmallRNAv1.5 Sample Prep Guide]
*[https://icom.illumina.com/Download/Summary/hJKJ92FSg0-ggFSSFSOnpQ SmallRNA Oligo Only Kit Sample Prep Guide]

<B><BIG> Genomic DNA </BIG></B>
*[https://icom.illumina.com/Download/Summary/5vvsqmSoGUKq8t6SBWjjyg Genomic Sample Prep]
*[https://icom.illumina.com/Download/Summary/vCFO-bin9ES31x5cPVYFmA Paired End Sample Prep Guide]
*[https://icom.illumina.com/Download/Summary/ABR93AL3D0eLNaP3P-yckw Multiplexing Sample Prep Guide]
*[https://icom.illumina.com/Download/Summary/LAJkz5z08kOWWwwsoZFjmg Mate Pair 2-5Kb Sample Prep Guide]

== [[BioMicroCenter:Microarray|AGILENT MICROARRAY PROTOCOLS]] ==

* [[BioMicroCenter:Microarray|aRNA Synthesis and Labeling]] - [[Media:aRNA_labeling.docx| DOWNLOAD]] <br>
* [[BioMicroCenter:Microarray|Hybridization]] - [[Media:hybridization.docx| DOWNLOAD]] <br>
* [[BioMicroCenter:Microarray|Wash and Scan]] - [[Media:wash_scan.docx| DOWNLOAD]]

== [[BioMicroCenter:Microarray|Caliper Information]] ==

* [[BioMicroCenter:Microarray|BioRad HSP dimensions]] - [[Media:BioRad HSP dimensions Feb 2011.pdf| DOWNLOAD]]