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BioMicroCenter:PricingFY2019
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== SEQUENCING == Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason. <BR><BR> === HISEQ2000 SEQUENCING === {| class="wikitable" border=1 ! HISEQ SEQUENCING !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]] !width=80| MIT !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !width=80| unit ! Notes |- |Single End 40nt |align="right"| $1,200 |align="right"| $1,320 |align="right"| $1,800 |align="center"| per lane | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1y. |- | Other read lengths |colspan="5" align=left |7/1/18: Custom only. Requires purchase of full flowcell. |} === NEXTSEQ500 SEQUENCING === {| class="wikitable" border=1 ! NEXTSEQ SEQUENCING !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]] !width=80| MIT !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !width=80| unit ! Notes |- |91nt kit <BR> (eg. 40PE or 75SE) |align="right"| $2,100 |align="right"| $2,320 |align="right"| $3,150 |align="center" rowspan="3"| per flowcell |rowspan="3"| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year |- |166nt kit <BR> (eg. 80PE or 150SE) |align="right"| $3,200 |align="right"| $3,520 |align="right"| $4,800 |- |316nt kit <BR> (eg. 150PE or 300SE) |align="right"| $4,900 |align="right"| $5,390 |align="right"| $7,350 |} === MISEQ SEQUENCING === {| class="wikitable" border=1 ! MISEQ SEQUENCING !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]] !width=80| MIT !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !width=80| unit ! Notes |- |70nt V2 kit <BR> (eg. 30PE or 70SE) |align="right"| $1,155 |align="right"| $1,270 |align="right"| $1,732 |align="center" rowspan=6| per lane |rowspan=6| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year |- |150nt V3 kits <BR> (eg. 75PE) |align="right"| $1,250 |align="right"| $1,375 |align="right"| $1,875 |- |300nt V2 kit<BR> (eg. 150PE) |align="right"| $1,365 |align="right"| $1,500 |align="right"| $2,050 |- |500nt V2 kit <BR> (eg. 250PE) |align="right"| $1,575 |align="right"| $1,732 |align="right"| $2,362 |- | 600nt V3 kit<BR> (eg. 300PE) |align="right"| $1,890 |align="right"| $2,080 |align="right"| $2,835 |- | 500nt '''NANO''' Run <BR> (eg. 250PE) |align="right"| $675 |align="right"| $742.50 |align="right"| $1,012.50 |} === OTHER ILLUMINA SERVICES === {| class="wikitable" border=1 ! ILLUMINA SUPPORTING SERVICES !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]] !width=80| MIT !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !width=80| unit ! Notes |- | Quality Control Only -only one QC is included per sequencing lane. |align="right"| $20 |align="right"| $26 |align="right"| $30 |align="center"| per sample | Includes RT-PCR and Frag.Analyzer |- | Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. |align="right"| $10 |align="right"| $13 |align="right"| $15 |align="center"| per sample | Includes Fluorometric quantification and Frag.Analyzer |} === PACBIO SEQUENCING === {| class="wikitable" border=1 ! SEQUEL SEQUENCING !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]] !width=80| MIT !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !width=80| unit ! Notes |- | 10 hour v3 Chemistry |align="right"| $1,275 |align="right"| $1,405 |align="right"| $1,915 |align="center" rowspan=2| per SMRTCell |rowspan=2| Includes polymerase binding and movie. |- | 20 hour v3 Chemistry |align="right"| $1,400 |align="right"| $1,540 |align="right"| $2,100 |} == LIBRARY GENERATION == === ILLUMINA LIBRARY - DNA === {| class="wikitable" border=1 ! ILLUMINA Sample Preparation !width=120| Batch Size !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]] !width=80| MIT !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] ! Notes |- ! colspan="3" | '''STANDARD THROUGHPUT DNA''' <BR> Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ! colspan="3" style="color: blue;" | '''HIGH THROUGHPUT DNA''' <BR> Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. |- |rowspan=3 |Standard Illumina Library (LM-PCR) <BR>NEB UltraII |align="center"| per sample |align="right"| $100 |align="right"| $110 |align="right"| $150 | Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA). |- style="color: blue;" |align="center"| 24 samples |align="right"| $750 |align="right"| $825 |align="right"| $1075 |rowspan=2 | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA). |- style="color: blue;" |align="center"| 96 samples |align="right"| $1500 |align="right"| $1650 |align="right"| $2250 |- | rowspan="4" | Tagmentation (NEXTERA-XT) - 1ng input |align="center"| ''1'' Sample |align="right"| $100 |align="right"| $110 |align="right"| $150 | rowspan="4" | Includes tagmentation, indexing, enrichment, and quality control (SYBR and FA). |- style="color: blue;" |align="center"| ''16'' <BR>(2 column)<BR> TECAN |align="right" | $1000 |align="right" | $1100 |align="right" | $1500 |- |align="center" style="color: blue;" | ''96'' <BR> Mosquito |align="right" style="color: blue;" | $1500 |align="right" style="color: blue;" | $1650 |align="right" style="color: blue;" | $2250 |- |align="center" style="color: blue;" | ''384'' <BR> Mosquito |align="right" style="color: blue;" | $3000 |align="right" style="color: blue;" | $3300 |align="right" style="color: blue;" | $4500 |- |rowspan="2" style="color: blue;" | 16S Amplicon |align="center"|48 samples <BR>(6 columns) |align="right" style="color: blue;" | $1,800 |align="right" style="color: blue;" | $1,980 |align="right" style="color: blue;" | $2,700 |rowspan="2" style="color: blue;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA). |- |align="center"|add'l 48 samples <BR>(6 columns) |align="right" style="color: blue;" | $600 |align="right" style="color: blue;" | $660 |align="right" style="color: blue;" | $900 |- |rowspan="2" style="color: blue;" | Other Amplicon |align="center" style="color: blue;" |48 samples <BR>(6 columns) |align="right" style="color: blue;" | $1,440 |align="right" style="color: blue;" | $1,584 |align="right" style="color: blue;" | $2,160 |rowspan="2" style="color: blue;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA). <BR> Samples *must* be submitted amplified with appropriate linkers. |- |align="center"|add'l 48 samples <BR>(6 columns) |align="right" style="color: blue;" | $600 |align="right" style="color: blue;" | $660 |align="right" style="color: blue;" | $900 |- ! colspan="6" | '''ADDITIONAL FEES''' <BR> Samples not in proper input format will be subject to these additional charges. |- |style="color: blue;" | High Throughput Setup |align="center" style="color: blue;" |48 samples |align="right" style="color: blue;" | $50 |align="right" style="color: blue;" | $55 |align="right" style="color: blue;" | $75 | |- |Sample Cleanup |align="center"|per sample |align="right"| $5 |align="right"| $5.5 |align="right"| $7.5 |SPRI cleanup of samples to remove impurities. |- |style="color: blue;" |Quantification |align="center" style="color: blue;" |per sample |align="right" style="color: blue;" | $5 |align="right" style="color: blue;" | $5.5 |align="right" style="color: blue;" | $7.5 |Quantification of samples (FA) prior to arraying. |} === ILLUMINA LIBRARY - RNA === {| class="wikitable" border=1 ! ILLUMINA Sample Preparation !width=120| Batch Size !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]] !width=80| MIT !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] ! Notes |- ! colspan="3" | '''STANDARD RNA''' <BR> Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ! colspan="3" style="color: blue;" | '''HIGH THROUGHPUT RNA''' <BR> Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included |- |rowspan="2" | Standard polyA based isolation <BR> Kapa mRNA Hyperprep |align="center"| per sample |align="right"| $150 |align="right"| $165 |align="right"| $225 |Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA). |- |align="center" style="color: blue;" | ''24'' <br> TECAN |align="right" style="color: blue;" | $2000 |align="right" style="color: blue;" | $2200 |align="right" style="color: blue;" | $3000 |Includes polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA). |- | rowspan="2" style="color: blue;" | High Throughput 3' Digital Gene Expression (HT3DGE) |align="center" style="color: blue;"| ''24'' <br> TECAN |align="right" style="color: blue;" | $1000 |align="right" style="color: blue;" | $1100 |align="right" style="color: blue;" | $1500 | rowspan="2" style="color: blue;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC. |- |align="center" style="color: blue;" | ''96'' <br> TECAN |align="right" style="color: blue;" | $4000 |align="right" style="color: blue;" | $4400 |align="right" style="color: blue;" | $6000 |- |Total RNA sequencing:Mammalian <BR> Kapa Hyperprep + RiboErase |align="center"| per sample |align="right"| $225 |align="right"| $247.5 |align="right"| $337.5 |rowspan=2| Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control. |- |Total RNA sequencing:Other <BR> Kapa mRNA Hyperprep + Illumina RiboZero |align="center"| per sample |align="right"| $250 |align="right"| $275 |align="right"| $375 |- |Low Input polyA+ <BR> Clontech SMARTseq v4 + NexteraXT |align="center"| ''1'' sample |align="right"| $250 |align="right"| $275 |align="right"| $375 | rowspan="3" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC. |- |rowspan="2"| Low Input polyA+ <BR> SMARTseq v2 + NexteraXT |align="center" style="color: blue;" | ''24'' <BR> Mosquito |align="right" style="color: blue;" | $1600 |align="right" style="color: blue;" | $1760 |align="right" style="color: blue;" | $2400 |- |align="center" style="color: blue;" | ''96'' <BR> Mosquito |align="right" style="color: blue;" | $2500 |align="right" style="color: blue;" | $2750 |align="right" style="color: blue;" | $3750 |- |Low Input Mammalian Total <BR> Clontech ZapR Kit |align="center"| ''1'' sample |align="right"| $200 |align="right"| $220 |align="right"| $300 | rowspan="1" | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA). |- | Tight Size Cut |align="center"| per pool |align="right"| $85 |align="right"| $94 |align="right"| $130 | +/- 40bp using PippinPrep |- | Small RNA | align="center"|per sample | align="right"|$275 | align="right"|$303 | align="right"|$413 | Uses NEB, BIOO or QIAGEN small RNA kit |- ! colspan="6" | '''ADDITIONAL FEES''' <BR> Samples not in proper input format will be subject to these additional charges. |- |style="color: blue;" | High Throughput Setup |align="center" style="color: blue;" |48 samples |align="right" style="color: blue;" | $50 |align="right" style="color: blue;" | $55 |align="right" style="color: blue;" | $75 | |- |Sample Cleanup |align="center"|per sample |align="right"| $5 |align="right"| $5.5 |align="right"| $7.5 |SPRI cleanup of samples to remove impurities. |- |style="color: blue;" |Quantification |align="center" style="color: blue;" |12 samples |align="right" style="color: blue;" | $5 |align="right" style="color: blue;" | $5.5 |align="right" style="color: blue;" | $7.5 |Quantification of samples (FA) prior to arraying. |- |} . === PACBIO LIBRARIES === {| class="wikitable" border=1 ! PacBio Sample Preparation !width=120| Batch Size !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]] !width=80| MIT !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] ! Notes |- |rowspan=2|SMRTBell Only<BR> eg:PCR products | Sample 1 || $300 || $330 || $450 |rowspan=2| Includes QC, repair and addition of indexed SMRTBells |- | Samples 2-8 || $150 || $165 || $225 |- |rowspan=2|Genomic Library | Sample 1 || $450 || $495 || $675 |rowspan=2| Includes Covaris gSPIN fragmentation, Pippin sizing, repair and SMRTbell as above |- | Sample 2-8 || $225 || $250 || $340 |} == SINGLE CELL SERVICES == === SEQWELL === {| class="wikitable" border=1 ! 10X USAGE !width=120| Batch Size !width=80|KI !width=80| [[BioMicroCenter:CoreDeps|OTHER CORE LAB]] !width=80| MIT !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] ! Notes |- |colspan=7 align="center"|'''Assisted Service''' |- |FULL SERVICE<BR> Begin with cells |per array|| - || - || - || - || Includes all steps, QC of cDNA<BR>Begins in KI Nanowell Core |- |FROM cDNA on BEADS |per sample || $350 || $350 || $385 || $525 | Includes exonuclease treatment, amplification, QC of cDNA and library production<BR> Must coordinate with BMC staff on submission. |- |FROM AMPLIFIED cDNA |per sample || $100 || $100 || $110 || $150 || Includes QC of cDNA and library production only. |- |colspan=7 align="center"|'''Reagents for SeqWell''' |- |SeqWell Array |colspan=6 rowspan="2" | contact shanu@mit.edu for pricing and availability |- |SeqWell beads |} === 10X CHROMIUM === {| class="wikitable" border=1 ! 10X USAGE !width=120| Batch Size !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]] !width=80| MIT !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] ! Notes |- |colspan=6 align="center"|'''Assisted Service''' |- |3'RNA (v3)|| per sample|| $1800 || $1980 || $2700 || Includes QC of cDNA and creation of Illumina Library |- |colspan=6 align="center"|'''Walk-up Service''' |- |3'RNA Beads and Reagents (v3)|| per samples || $1350 || $1485 || $2025 || |- |ATACseq Beads and Reagents* || per sample || $1280 || $1408 || $1920 || |- |scCNV Beads and Reagents* || per 4 samples || $1330 || $1463 || $1995 || |- |Chips (up to 8 sample) || per chip || $350 || $385 || $525 || Includes service contract charge. |- |Training<BR>Limited to 2 people |per method & per session<BR> Reagents not included || $500 || $550 || $750 || |} *please let BMC know if you plan to run this method early so we will have reagents ready for you. === NAMOCELL === {| class="wikitable" border=1 ! NAMOCELL !width=120| Batch Size !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]] !width=80| MIT !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] ! Notes |- |Cartridge|| 1 cartridge per sample|| $75 || $85 || $125 || Includes service contract charge |} == OTHER GENOMICS SERVICES == <br> === [[BioMicroCenter:QC|QUALITY CONTROL]] === {| class="wikitable" border=1 ! QUALITY CONTROL !width=100| unit !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]] !width=100| MIT !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] ! Notes |- |Fragment Analyzer: RNA or DNA |per sample ||$8 || $9 || $12 | |- |Fragment Analyzer: Preloaded row | per row (12) ||$30 ||$33 || $45 | 2ul per sample in each well using plates from BMC |- |FemtoPulse: RNA or DNA |per sample || $10 || $11 || $15 | |- |FemtoPulse: Preloaded Row |per row (12) || $50 || $55 || $75 | 2ul per sample in each well using plates from BMC |- |BioAnalyzer: Assisted Service (RNA or DNA) |per sample || $15 || $18 || $25 | |- |BioAnalyzer: Assisted Service (High Sensitivity protein) |per sample || $40 || $44 || $60 | detection of proteins at < 30ng/ul. |- |} === [[BioMicroCenter:MicroArrays|MICROARRAYS]] === {| class="wikitable" border=1 ! MICROARRAYS !width=100| unit !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]] !width=100| MIT !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] ! Notes |- | AGILENT SCANNER |align="center"| per slide |align="right"| $50 |align="right"| $50 |align="right"| N/A | Price per glass slide. |- |} Agilent scanning services will end 6/30/2019 <br>. == [[BioMicroCenter:RTPCR|REAL TIME PCR]] == {| border=1 ! OTHER LAB SERVICES !width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !width=100| unit ! SIGN UP ! Notes |- | Roche LightCycler 480 |align="right"| $10 |align="right"| N/A |align="center"| per plate | [[BioMicroCenter:Forms#Calenders|CALENDAR]] *available for CORE only | Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training |- | SYBR Green (KAPA/Roche) |align="right"| $25 |align="right"| N/A |align="center"| per ml | | Available for MIT only |- | 96-well PLATES |align="right"| $6 |align="right"| N/A |align="center"| per plate | | Available for MIT only |- |} . <br> == OTHER LAB SERVICES == {| border=1 ! OTHER LAB SERVICES !width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !width=100| unit ! SIGN UP ! Notes |- |colspan=6 align="center"| '''COVARIS SONICATOR''' |- |COVARIS E220 |align="right"|$20 |align="right"|$30 |per hour |[https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] |tubes available for purchase. |- | colspan="6" align="center"| '''PIPPIN PREP''' |- |Gel Isolation |align="right"| $50 |align="right"| $75 |align="center"| per gel (5 lanes) | TBA | |- |Training |align="right"| $25 |align="right"| $50 |align="center"| per session | TBA | Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training. |- | colspan="6" align="center"| '''PLATE READER''' |- | Varioskan |align="right"| $18 |align="right"| $27 |align="center"| per hour | [[BioMicroCenter:Forms#Calenders|CALENDAR]] | Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training |- | colspan="6" align="center"| '''ROBOTICS''' |- | Tecan EVO 150 |align="right"| $50 |align="right"| $55 |align="center"| per 1h block - based on scheduled time. | [[BioMicroCenter:Forms#Calenders|CALENDAR]] | |- |non-filter tips |align="right"| $6.00 |align="right"| $6.00 |align="center"| per box | |** Please let us know if there is additional plasticware you would like us to stock. |- |Filter tips |align="right"| $8.00 |align="right"| $8.00 |align="center"| per box | | |- | Training |align="right"| $50 |align="right"| $55 |align="center"| per session | Contact [[BioMicroCenter:People|Stuart]] | |- | colspan="6" align="center"| '''NANODROP''' |- | Nanodrop |align="right"| -- |align="right"| -- |align="center"| | Walk up service | * no charge |- |} <br><br> == COMPUTATIONAL SERVICES == {| class="wikitable" border=1 ! COMPUTATIONAL SERVICES !width=200| unit !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]] !width=100| Other MIT / non-MIT ! Notes |- | Informatics Support | align="right"| per hour | align="right"| $80 | align="right"| $110 / $150 | Very limited support for non-MIT users |- | Informatics Share | align="right"| per 0.1FTE | align="right"| $1,100 | align="right"| N/A | MIT only. Priority access to informatics. |- |Active Data storage |align="right"| per TB per year |align="right"| $160 |align="right"| $240 / N/A | |- | Archival Storage (read only. Unmutable) |align="right"| per TB per year |align="right"| $80 |align="right"| $120 / N/A | |- | Tape Archive / Recovery |align="right"| per tape |align="right"| $60 |align="right"| $70 | |}
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