Editing BioMicroCenter:Pricing

{{BioMicroCenter}}

== SEQUENCING - HIGH OUTPUT ==
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason. <BR><BR>

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===
{| class="wikitable" border=1 
 ! NOVASEQ SEQUENCING
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=80| unit
 ! Notes
 |-
 |NovaSeq SP 100nt
 |align=right| $3,699
 |align=right| $4,068
 |align=right| $5,178
 |rowspan=9| Per flowcell
 |rowspan=9| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
 |-
 |NovaSeq SP 300nt** 
 |align=right| $5,238
 |align=right| $5,761
 |align=right| $7,333.20
 |-
 |NovaSeq SP 500nt
 |align=right| $7,020
 |align=right| $7,722
 |align=right| $9,828
 |-
 |NovaSeq S1 100nt**
 |align=right| $5,832
 |align=right| $6,415.20
 |align=right| $8,164.80
 |-
 |NovaSeq S1 200nt**
 |align=right| $7,452
 |align=right| $8,197.20
 |align=right| $10,432
 |-
 |NovaSeq S1 300nt**
 |align=right| $8,127
 |align=right| $8,939.70
 |align=right| $11,377.80
 |-
 |NovaSeq S2 100nt**
 |align=right| $10,395
 |align=right| $11,434.50
 |align=right| $14,553
 |-
 |NovaSeq S2 200nt**
 |align=right| $12,960
 |align=right| $14,256
 |align=right| $18,144
 |-
 |NovaSeq S2 300nt**
 |align=right| $13,500
 |align=right| $14,850
 |align=right| $18,900
 |-
 |NovaSeq S4 50nt 
 |align=right| $3,780
 |align=right| $4,158
 |align=right| $5,292
 |rowspan=2| Per Lane
 |rowspan=2| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
 |-
 |NovaSeq S4 300nt
 |align=right| $5,400
 |align=right| $5,940
 |align=right| $7,560
 |-
 |NovaSeqX 10b 300nt LANE^^
 |colspan=3| pricing in ilabs
 |rowspan=2| Per Lane
 |rowspan=3| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
 |-
 |NovaSeqX 25b 300nt LANE^^
 |colspan=3| pricing in ilabs
 |-
 |NovaSeqX 25b 100nt Flowcell^^
 |colspan=3| pricing in ilabs
 | Per Flowcell
 |-
|}
 ** - minimal inventory maintained
 ^^ - through collaboration with nearby academic shared resources

== SEQUENCING - MID OUTPUT ==
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason. <BR><BR>
=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===
{| class="wikitable" border=1 
 ! ELEMENT AVITI SEQUENCING
 !width=80| Lane/Flowcell
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=80| unit
 ! Notes
 |-
 |rowspan=2| 75 PE
 | Full flowcell (2 lane, 800m read)
 |align="right"| $1,900
 |align="right"| $2,090
 |align="right"| $2,660
 |align="center"| per flowcell
 |rowspan=5| Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
 |-
 |One lane (~400m read)
 |align="right"| $1,000
 |align="right"| $1,100
 |align="right"| $1,400
 |align="center"| per lane
 |-
 |rowspan=2| 150 PE
 | Full flowcell (2 lane, ~800m read)
 |align="right"| $2,650
 |align="right"| $2,915
 |align="right"| $3,710
 |align="center"| per flowcell
 |-
 |One lane (~400m read)
 |align="right"| $1,375
 |align="right"| $1,513
 |align="right"| $1,925
 |align="center"| per lane
 |-
 | 300 PE
 | Full flowcell (~200m reads)
 |align="right"| $3,500
 |align="right"| $3,850
 |align="right"| $4,900
 |align="center"| per lane
|}
=== NEXTSEQ500 SEQUENCING ===
{| class="wikitable" border=1 
 ! NEXTSEQ SEQUENCING
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=80| unit
 ! Notes
 |-
 |91nt kit <BR> (eg. 40PE or 75SE)
 |align="right"| $2,570
 |align="right"| $2,827
 |align="right"| $3,598
 |align="center" rowspan="3"| per flowcell
 |rowspan="3"| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year 
 |-
 |166nt kit <BR> (eg. 80PE or 150SE)
 |align="right"| $4,375
 |align="right"| $4,812.50
 |align="right"| $6,125
 |-
 |316nt kit <BR> (eg. 150PE or 300SE)
 |align="right"| $6,420
 |align="right"| $7,062
 |align="right"| $8,988
|}

== SEQUENCING - LOW OUTPUT ==
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason. <BR><BR>
=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===
{| class="wikitable" border=1 
 ! SINGULAR G4 SEQUENCING
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=80| unit
 ! Notes
 |-
 |50 PE: F3 Lane (~100m reads)
 |align="right"| $300
 |align="right"| $330
 |align="right"| $420
 |align="center" | per lane
 |rowspan=3| Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
 |-
 |Rapid 100nt: F3 flowcell (~400m reads)*
 |align="right"| $1200
 |align="right"| $1321
 |align="right"| $1680
 |align="center"| per flowcell
 |-
 |150 PE: F3 Lane (~100m reads)
 |align="right"| $450
 |align="right"| $495
 |align="right"| $630
 |align="center"| per lane

|}
 * Rapid flowcells are required for adapted Illumina libraries.

=== MISEQ SEQUENCING ===
{| class="wikitable" border=1 
 ! MISEQ SEQUENCING
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=80| unit
 ! Notes
 |-
 |70nt V2 kit <BR> (eg. 30PE or 70SE)
 |align="right"| $1,320
 |align="right"| $1,452
 |align="right"| $1,848
 |align="center" rowspan=6| per lane
 |rowspan=6| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
 |-
 |150nt V3 kits <BR> (eg. 75PE)
 |align="right"| $1,430
 |align="right"| $1,573
 |align="right"| $2,002
 |-
 |300nt V2 kit<BR> (eg. 150PE)
 |align="right"| $1,595
 |align="right"| $1,754.50
 |align="right"| $2,233
 |-
 |500nt V2 kit <BR> (eg. 250PE)
 |align="right"| $1,760
 |align="right"| $1,936
 |align="right"| $2,464
 |-
 |600nt V3 kit<BR> (eg. 300PE)
 |align="right"| $2,200
 |align="right"| $2,420
 |align="right"| $3,080
 |-
 |500nt '''NANO''' Run <BR> (eg. 250PE)
 |align="right"| $770
 |align="right"| $847
 |align="right"| $1,078
|}


=== OTHER SHORT READ SERVICES ===
{| class="wikitable" border=1 
 ! SHORT READ SUPPORTING SERVICES
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=80| unit
 ! Notes
 |-
 | Quality Control Only -only one QC is included per sequencing lane.
 |align="right"| $35
 |align="right"| $38.50
 |align="right"| $49
 |align="center"| per sample
 | Includes RT-PCR and Frag.Analyzer
 |-
 | Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling.
 |align="right"| $15
 |align="right"| $16.50
 |align="right"| $21
 |align="center"| per sample
 | Includes Fluorometric quantification and Frag.Analyzer
 |-
 |qPCR only (short read)
 |align="right"| $25
 |align="right"| $27.50
 |align="right"| $35
 |align="center"| per sample
 | Includes qPCR values from standard QC only
 |-
 |qPCR only - full plate(short read)
 |align="right"| $400
 |align="right"| $440
 |align="right"| $560
 |align="center"| per sample
 | excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable" border=1 
 ! PROMETHION SEQUENCING
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=80| unit
 ! Notes
 |-
 | 72 hour Flowcell 
 |align="right"| $1,200
 |align="right"| $1,320
 |align="right"| $1,680
 |align="center"|per Flowcell
 ||Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable" border=1 
 ! PACBIO REVIO SEQUENCING^^
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=80| unit
 ! Notes
 |-
 | 72 hour Flowcell 
 |colspan=3 | pricing in ilabs
 |align="center"|per Flowcell
 ||Fastq stored for 1y. Trace data stored only 30d.
|}

 ^^ - through collaboration with nearby academic shared resources

== LIBRARY GENERATION ==
=== SHORT READ LIBRARY - CONVERSION ===
{| class="wikitable" border=1
 !width=100| Input library
 !width=100| Convert To
 !width=100| Method
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 ! Notes
 |-
 |align="center"|Illumina
 |align="center"|Singular
 |align="center"|Index Overwrite (per sample)
 |align="right"| $30
 |align="right"| $33
 |align="right"| $42
 | Includes QC and reindexing
 |-
 |align="center"|Illumina
 |align="center"|Singular
 |align="center"|P5/P7 conversion (per pool)
 |align="right"| $100
 |align="right"| $110
 |align="right"| $140
 | Includes QC. Samples not reindexed <BR><B> Requires full flowcell for sequencing (Rapid) </B>
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable" border=1
 !width=160| ILLUMINA Sample Preparation
 !width=60| Type
 !width=60| Batch Size
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 ! Notes
 |-
 ! colspan="4" | '''STANDARD THROUGHPUT DNA''' <BR> Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
 ! colspan="3" style="color: blue;" | '''HIGH THROUGHPUT DNA''' <BR> Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
 |-
 |rowspan=3 |LIGATION BASED<BR>NEB UltraII
 |align="center"|STD
 |align="center"| per sample
 |align="right"| $105
 |align="right"| $115
 |align="right"| $157
 | Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
 |- style="color: blue;"
 |align="center" rowspan=2|HTL
 |align="center"| 24
 |align="right"| $900
 |align="right"| $990
 |align="right"| $1,260
 |rowspan=2 | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
 |- style="color: blue;"
 |align="center"| 96
 |align="right"| $1,800
 |align="right"| $1,980
 |align="right"| $2,520
 |-

 |Tagmentation <BR> NEXTERA-XT
 |align="center" rowspan=2|STD
 |align="center" rowspan=2|per Sample
 |align="right"| $105
 |align="right"| $115
 |align="right"| $157
 | rowspan="2" | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA). 
 |-
 |Tagmentation <BR> NEXTERA-FLEX
 |align="right"| $157
 |align="right"| $173
 |align="right"| $236
 
 |- style="color: blue;"
 |rowspan=2 | NEXTERA-XT OR FLEX
 |rowspan=2 align="center"| HTL
 |align="center"| ''24''
 |align="right"| $900
 |align="right"| $990
 |align="right"| $1,260
 |rowspan=2 | Includes tagmentation, indexing, enrichment, and quality control spotcheck(SYBR and FA).
 |-
 |align="center" style="color: blue;" | ''96''
 |align="right" style="color: blue;" | $1,800
 |align="right" style="color: blue;" | $1,980
 |align="right" style="color: blue;" | $2,520
 
 |-style="color: blue;"
 |rowspan="2"  | 16S Amplicon
 |align="center" rowspan=2 | HTL
 |align="center"|48
 |align="right" | $1,900
 |align="right" | $2,090
 |align="right" | $2,660
 |rowspan="2"   | Includes normalization, enrichment, pooling and quality control (SYBR and FA). 
 |-style="color: blue;"
 |align="center"|add'l 48
 |align="right" | $650
 |align="right" | $715
 |align="right" | $910

 |-style="color: blue;"
 |rowspan="2"   | Other Amplicon
 |rowspan=2 align="center"| HTL
 |align="center" |48
 |align="right" | $1,600
 |align="right" | $1,760
 |align="right" | $2,240
 |rowspan="2"   | Includes normalization, enrichment, pooling and quality control (SYBR and FA). <BR> Samples *must* be submitted amplified with appropriate linkers.
 |- style="color: blue;" 
 |align="center"|add'l 48
 |align="right" | $630
 |align="right" | $693
 |align="right" | $945
 |-
 ! colspan="7" | '''ADDITIONAL FEES''' <BR> Samples not in proper input format will be subject to these additional charges.
 |-
 |style="color: blue;" colspan=2| High Throughput Setup
 |align="center" style="color: blue;" |48 samples 
 |align="right" style="color: blue;" | $60
 |align="right" style="color: blue;" | $66
 |align="right" style="color: blue;" | $84
 | 
 |-
 |colspan=2|Sample Cleanup
 |align="center"|per sample
 |align="right"| $5
 |align="right"| $5.50
 |align="right"| $7
 |SPRI cleanup of samples to remove impurities. 
 |-
 |style="color: blue;" colspan=2|Quantification
 |align="center" style="color: blue;" |per sample
 |align="right" style="color: blue;" | $10
 |align="right" style="color: blue;" | $11
 |align="right" style="color: blue;" | $14
 |Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable" border=1
 !width=160| ILLUMINA Sample Preparation
 !width=60| Type
 !width=60| Batch Size
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 ! Notes
 |-
 ! colspan="4" | '''STANDARD RNA''' <BR> Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
 ! colspan="3" style="color: blue;" | '''HIGH THROUGHPUT RNA''' <BR> Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
 |-
 |rowspan="3"|polyA based isolation <BR><BR> NEB UltraII RNAseq
 |align="center"|<B>STD</B>
 |align="center"| per sample
 |align="right"| $157
 |align="right"| $173
 |align="right"| $236
 |Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
 |-
 |rowspan=2 align="center" style="color: blue;"| <B>HTL</b><BR>(NEB only)
 |align="center" style="color: blue;" | ''24''
 |align="right" style="color: blue;" | $1,500
 |align="right" style="color: blue;" | $1,650
 |align="right" style="color: blue;" | $2,100
 |Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
 |-
 |align="center" style="color: blue;" | ''96''
 |align="right" style="color: blue;" | $3,000
 |align="right" style="color: blue;" | $3,300
 |align="right" style="color: blue;" | $4,200
 |Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
 |-
 
 |rowspan="2"| High Throughput 3' Digital Gene Expression (HT3DGE)
 |rowspan=2  align="center" style="color: blue;"| <B>HTL</b>
 |align="center" style="color: blue;"| ''24'' 
 |align="right" style="color: blue;" | $1,050
 |align="right" style="color: blue;" | $1,155
 |align="right" style="color: blue;" | $1,470
 | rowspan="2"  style="color: blue;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
 |-
 |align="center" style="color: blue;" | ''96''
 |align="right" style="color: blue;" | $2,100
 |align="right" style="color: blue;" | $2,310
 |align="right" style="color: blue;" | $2,940
 |-
 
 |rowspan="3"|Total RNA sequencing <BR><BR> NEB RNAseq +Ribodepletion
 |align="center"|<B>STD</B>
 |align="center"| per sample
 |align="right"| $235
 |align="right"| $258.5
 |align="right"| $352.5
 |Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control. 
 |-
 |rowspan=2 align="center" style="color: blue;"| <B>HTL</b><BR>(NEB only, covaris RNA fragmentation)
 |align="center" style="color: blue;" | ''24''
 |align="right" style="color: blue;" | $2,500
 |align="right" style="color: blue;" | $2,750
 |align="right" style="color: blue;" | $3,500
 |rowspan=2|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
 |-
 |align="center" style="color: blue;" | ''96''
 |align="right" style="color: blue;" | $5,000
 |align="right" style="color: blue;" | $5,500
 |align="right" style="color: blue;" | $7,000
 |-
 
 |rowspan=3|Low Input polyA+ <BR> Clontech SMARTseq + NexteraXT or Flex
 |align="center"| '''STD'''<BR>v4
 |align="center"|per sample
 |align="right"| $262
 |align="right"| $289
 |align="right"| $394
 | rowspan="3" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
 |-
 |rowspan="2" align="center" style="color: blue;"| '''HTL'''<br>v2
 |align="center" style="color: blue;" | ''24''
 |align="right" style="color: blue;" | $2,000
 |align="right" style="color: blue;" | $2,200
 |align="right" style="color: blue;" | $2,800
 |-
 |align="center" style="color: blue;" | ''96''
 |align="right" style="color: blue;" | $3,200
 |align="right" style="color: blue;" | $3,520
 |align="right" style="color: blue;" | $4,480
 |-
 
 |rowspan="3"|Low Input Mammalian Total <BR> Clontech ZapR Kit
 |align="center"| STD
 |align="center"| per sample
 |align="right"| $210
 |align="right"| $231
 |align="right"| $315
 | rowspan="1" | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA). 
 |-
 |rowspan=2 align="center" style="color: blue;"| <B>HTL</b><BR>(covaris RNA fragmentation)
 |align="center" style="color: blue;" | ''24''
 |align="right" style="color: blue;" | $3000
 |align="right" style="color: blue;" | $3300
 |align="right" style="color: blue;" | $4200
 |rowspan=2|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
 |-
 |align="center" style="color: blue;" | ''96''
 |align="right" style="color: blue;" | $5000
 |align="right" style="color: blue;" | $5500
 |align="right" style="color: blue;" | $7000
 |-
 | Small RNA
 |align="center"| STD
 | align="center"|per sample
 | align="right"|$289
 | align="right"|$318
 | align="right"|$433
 | Uses NEB, BIOO or QIAGEN small RNA kit
 |-
 ! colspan="7" | '''ADDITIONAL FEES''' <BR> Samples not in proper input format will be subject to these additional charges.
 |-
 |colspan=2 style="color: blue;" | High Throughput Setup
 |align="center" style="color: blue;" |48 samples 
 |align="right" style="color: blue;" | $60
 |align="right" style="color: blue;" | $66
 |align="right" style="color: blue;" | $84
 | 
 |-
 |colspan=2|Sample Cleanup
 |align="center"|per sample
 |align="right"| $5
 |align="right"| $5.50
 |align="right"| $7
 |SPRI cleanup of samples to remove impurities. 
 |-
 |colspan=2 style="color: blue;" |Quantification
 |align="center" style="color: blue;" |per sample
 |align="right" style="color: blue;" | $10
 |align="right" style="color: blue;" | $11
 |align="right" style="color: blue;" | $14
 |Quantification of samples (FA) prior to arraying.
 |-
|}

.

=== LONG READ LIBRARIES ===
{| class="wikitable" border=1
 !Oxford Nanopore Sample Preparation
 !width=120| Nanopore Kit
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 ! Notes
 |-
 |Standard Ligation || LSK || $160 || $176 || $240 
 |rowspan=6|Oxford Nanopore prep methods remain highly experimental and are continually in flux. <BR> As such, we can only estimate cost based on reagents/effort and cannot guarantee results.<BR> Please consult the core prior to sample submission for the latest information.
 |-
 |Native Barcoding <br> (ligation indexing) || - || $50 || $55 || $70
 |-
 |SMARTseq -> Ligation || Cv4+LSK || $300 || $330 || $420
 |-
 |Rapid Prep (tagment - ~10-20kb) || RAD || $150 || $165 || $225
 |-
 |Long Insert Prep || RAD (modified, target >100kb) || $262 || $289 || $393 
 |-
 |Direct RNA || RNA004 || $160 || $176 || $240 
|}

== SAMPLE EXTRACTION ==
=== CHEMAGIC360 ===
{| class="wikitable" border=1
 !TYPE
 !width=120| Batch Size
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 ! Notes
 |-
 |DNA
 |96
 |$700
 |$770
 |$980
 |
 |-
 |RNA
 |96
 |$800
 |$880
 |$1120
 |
|}

== SINGLE CELL & SPATIAL SERVICES ==
=== 10X CHROMIUM ===
{| class="wikitable" border=1
 ! 10X USAGE
 !width=120| Batch Size
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 ! Notes
  |-
 |colspan=6 align="center"|'''Assisted Service'''
 |- 
 |colspan=6 |''DIGITAL GENE EXPRESSION''
 |- 
 |rowspan=2 |5' RNA <br> 3' RNA
 |setup || $1,015 || $1,116.50 || $1,421 || charge per submission
 |- 
 |per sample|| $1,750 || $1,925 || $2,450  || Includes QC of cDNA and creation of Short Read Library
 |- 
 |rowspan=2 |5' RNA <br> 3' RNA - on chip multiplexing
 |setup || $1,150 || $1,265 || $1,1610 || charge per submission
 |- 
 |per 4 samples|| $2,305 || $2,535 || $3,227  || Includes QC of cDNA and creation of Short Read Library
 |- 
 |rowspan=2 |Fixed RNA <br> probe based. human/mouse only
 |setup || $1300 || $1430 || $1820 || charge per submission
 |- 
 |per sample (4plex) || $3900|| $4290 || $5460 || Includes reagents for hashing of 4 samples in one channel.<br>QC of cDNA and creation of Short Read Library
 |-
 |rowspan=2 |Amplicon (CITE-SEQ, TCR, BCR, antibody barcocde, etc // per amplicon)
 |setup || $470 || $517 || $658 || additional prep beyond DGE
 |- 
 |per sample|| $45 || $50 || $63  || Includes QC of cDNA and creation of Short Read Library
 |-
 |colspan=6 |''scATACseq''
 |- 
 |rowspan=2 |ATACseq
 |setup || $880 || $968 || $1,232 || charge per submission
 |- 
 |per sample|| $1,675|| $1,843|| $2,345|| Includes QC and creation of Short Read Library
 |-
 |colspan=6 |''Multiome (ATAC+3'RNA)''
 |- 
 |rowspan=2 |MULTI
 |setup || $1,800|| $1,980|| $2,520|| charge per submission
 |- 
 |per sample|| $3,100|| $3,410|| $4,340|| Includes QC of cDNA and creation of Short Read Library
 |-
 |colspan=6 |''CANCELLATION''
 |-
 |per session || || $200|| $220|| $280|| <24h notice
|}
*please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===
{| class="wikitable" border=1
 ! SLIDES
 !width=120| Batch Size
 !width=80| KI Only
 !width=80| [[BioMicroCenter:CoreDeps|Other CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 ! Notes
 |- 
 |Test Array ||slide|| $1,300|| $1,511|| $1,607|| $2435|| recommended 1x per tissue type for fresh frozen
 |- 
 |Sample Slide - Fresh Frozen ||1 slide / 4 tiles|| $6,210|| $6,340|| $6,940|| $9,067 || Includes H&E stain images + Library Generation
 |- 
 |Sample Slide - FFPE (hs/mm only) ||1 slide / 2 tiles|| $4,550|| $4,550|| $4,996|| $6,359 || Includes library generation only.
 |- 
 |Sample Slide - HD (hs/mm only) ||1 slide / 2 tiles|| $7,850|| $7,850|| $8,596|| $10,940 || Includes library generation only.
|}

=== AVITI24 ===
{| class="wikitable" border=1
 ! array
|}

== OTHER GENOMICS SERVICES ==

<br>

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===
{| class="wikitable" border=1
 ! QUALITY CONTROL
 !width=100| unit
 !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=100| MIT 
 !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 ! Notes
 |-
 |Fragment Analyzer: RNA or DNA
 |per sample ||$15 || $16.50 || $21
 |
 |-
 |Fragment Analyzer: Preloaded row
 | per row (12) ||$80||$88 || $112
 | 2ul per sample in each well using plates from BMC
 |-
 |FemtoPulse: RNA or DNA
 |per sample || $30 || $33 || $42
 | 
 |-
 |FemtoPulse: Preloaded Row
 |per row (12) || $160 || $176 || $224
 | 2ul per sample in each well using plates from BMC
 |-
 |BioAnalyzer: Walkup Service 
 |per sample || $50 || $55 || $70
 |  
 |-
 |qPCR only (Illumina quantification test)
 |per sample || $25 || $27.50 || $35
 | 4 point serial dilution
 |-
 |Full plate qPCR (Illumina quantification test)
 |per plate || $400 || $440 || $560
 | full plate only (exclude column 1 for standards) - must be to BMC specifications.
 |-
|}

=== OLIGO SYNTHESIS ===
{| class="wikitable" border=1
 !Platform
 !width=100| Service
 !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=100| MIT 
 !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 ! Notes
 |-
 |rowspan=3| Dr.Oligo
 |Assisted
 |$2000<br>+consumables
 |$2200<br>+consumables
 |$2800<br>+consumables
 |includes setup and run of the instrument only. Large reagents / N2 included.
 |-
 |Walkup
 |$1000<br>+consumables
 |$1100<br>+consumables
 |$1400<br>+consumables
 |includes run of the instrument only. Large reagents / N2 included.
 |-
 |Training
 |$4000
 |$4400
 |$5600
 |ncludes two instrument runs. 
 |-
 |rowspan=6| DNAscript Syntax
 |Setup (per run)
 |$1000
 |$1100
 |$1400
 |Per run cost
 |-
 |2000 nucleotide synthesis
 |$500
 |$550
 |$700
 |Up to 96 samples
 |-
 |8000 nucleotide synthesis
 |$1500
 |$1650
 |$2100
 |Up to 96 samples
 |-
 |add Biotin (max48)
 |$115
 |$126
 |$161
 |rowspan = 3| max 48 per run and 2 per oligo. Couple to T only.
 |-
 |add Fluor
 |$300
 |$330
 |$420
 |-
 |add quench
 |$100
 |$110
 |$140
|}

== [[BioMicroCenter:RTPCR|REAL TIME PCR]] ==

{| border=1
 ! OTHER LAB SERVICES
 !width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]
 !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=100| unit
 ! SIGN UP
 ! Notes
 |-
 | Roche LightCycler 480
 |align="right"| $15
 |align="right"| N/A
 |align="center"| per plate
 | [[BioMicroCenter:Forms#Calenders|CALENDAR]] *available for CORE only
 | Contact [[BioMicroCenter:People|Christopher Hallee]] for training
 |-
 | SYBR Green (KAPA/Roche)
 |align="right"| $45
 |align="right"| N/A
 |align="center"| per ml
 |
 | Available for MIT only 
 |-
 | 96-well PLATES
 |align="right"| $10
 |align="right"| N/A
 |align="center"| per plate
 |
 | Available for MIT only
 |-
|}
.
<br>

== OTHER LAB SERVICES ==
{| border=1
 ! OTHER LAB SERVICES
 !width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]
 !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=100| unit
 ! SIGN UP
 ! Notes
 |- 
 |colspan=6 align="center"| '''COVARIS SONICATOR'''
 |-
 |COVARIS E220
 |align="right"|$20
 |align="right"|$30
 |per hour
 |[https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar]
 |tubes available for purchase.
 |-

 | colspan="6" align="center"| '''PIPPIN PREP'''
 |-
 |Gel Isolation
 |align="right"| $75
 |align="right"| $105
 |align="center"| per gel (5 lanes)
 | TBA
 | 
 |-
 |Training
 |align="right"| $25
 |align="right"| $50
 |align="center"| per session
 | TBA
 | Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
 |-
| colspan="6" align="center"| '''PLATE READER'''
 |-
 | Varioskan
 |align="right"| $18
 |align="right"| $27
 |align="center"| per hour
 | [[BioMicroCenter:Forms#Calenders|CALENDAR]]
 | Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training 
 |-
 | colspan="6" align="center"| '''ROBOTICS'''
 |-
 | Tecan EVO 150
 |align="right"| $75
 |align="right"| $105
 |align="center"| per 1h block - based on scheduled time.
 | [[BioMicroCenter:Forms#Calenders|CALENDAR]]
 | 
 |-
 |non-filter tips
 |align="right"| $8
 |align="right"| $11.20
 |align="center"| per box
 |
 |** Please let us know if there is additional plasticware you would like us to stock.
 |-
 |Filter tips
 |align="right"| $12.00
 |align="right"| $16.80
 |align="center"| per box
 |
 |
 |-
 | Training
 |align="right"| $65
 |align="right"| $91
 |align="center"| per hour
 | Contact [[BioMicroCenter:People|Tatum Murdock]]
 | 
 |-
 | colspan="6" align="center"| '''NANODROP'''
 |-
 | Nanodrop
 |align="right"| --
 |align="right"| --
 |align="center"| 
 | Walk up service
 | * no charge
 |-
|}
<br><br>

== COMPUTATIONAL SERVICES ==   
{| class="wikitable" border=1
 ! COMPUTATIONAL SERVICES
 !width=200| unit
 !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=100| Other MIT / non-MIT 
 ! Notes
  |-
 | Informatics Support
 | align="right"| per hour
 | align="right"| $90
 | align="right"| $125 / $205
 | Very limited support for non-MIT users
 |-
 | Informatics Share
 | align="right"| per 0.1FTE
 | align="right"| $1,250
 | align="right"| N/A
 | MIT only. Priority access to informatics.
 |-
 |Ingenuity Pathway Analysis
 |align="right"|per dataset
 |align="right" | $100
 |align="right" | $110/$150
 | Recovery of licence cost.
 |-
 |Active Data storage
 |align="right"| per TB per year
 |align="right"| $100
 |align="right"| $300 / N/A
 |  
 |-
 | Tape Archive / Recovery
 |align="right"| per tape
 |align="right"| $60
 |align="right"| $70
 | 
 |-
 | Informatics Training Classes
 |align="right"| per session
 |align="right"| $50
 |align="right"| $100
 | 
|}


OLD<BR>
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]]<BR>
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]]<BR>
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]]<BR>
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]]<BR>
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]]<BR>
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]]<BR>
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]]<BR>
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]]<BR>
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]]<BR>
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]]<BR>
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]]<BR>
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]]<BR>
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]