Editing BioMicroCenter:PREP TEST

{{BioMicroCenter}}
=Library Preparation Services=
[[Image:BMC_samplePrep_pipes.png|thumb|right|400px|Sample Preparation available in BioMicro]]
Generating high-quality data on the Illumina sequencing platform requires high-quality libraries. The BMC currently offers library preparation services for a variety of starting materials. Prior to sequencing, all samples must pass the BioMicro Center’s Sequencing Quality Control process, which verifies selection of inserts of a desired size and correct ligation of Illumina adapters.

==Illumina Library Prep Services==
Please follow the links in the table below for more information about our library preparation offerings.
{| class="wikitable" border=1
 |-
 ! style="text-align: center;" | METHOD TYPE
 ! style="text-align: center;" | DNA
 ! style="text-align: center;" | RNA
 |-
 | style="text-align: center; height: 2em; font-size: 16px;" | STANDARD
 | style="text-align: left; vertical-align: top; height: 2em;" | '''Ligation-mediated PCR Methods'''
* [[BioMicroCenter:DNA_LIB#NEB_Ultra_II | NEB Ultra II]]
'''Tagmentation Methods'''
* [[BioMicroCenter:DNA_LIB#Nextera_Flex | Nextera Flex]]
* [[BioMicroCenter:DNA_LIB#Nextera_XT | Nextera XT]]
 | style="text-align: left; height: 2em;" | '''Standard Input'''
* [[BioMicroCenter:RNA_LIB | NEB Ultra II Directional RNA]] with: <br> &nbsp;&nbsp; [[BioMicroCenter:RNA_LIB#NEB_Ultra_II_Directional_RNA_with_Poly(A)_Selection | Poly(A) mRNA Isolation]] <br> &nbsp;&nbsp; [[BioMicroCenter:RNA_LIB#NEB_Ultra_II_Directional_RNA_with_rRNA_Depletion_.28H.2FM.2FR.29 | rRNA Depletion (H/M/R)]] <br> &nbsp;&nbsp; rRNA Depletion (Bacterial)
'''Low Input'''
* [[BioMicroCenter:RNA_LIB#Clontech_SMARTseq_Low-Input | Clontech SMART-Seq v4]] <br> &nbsp;&nbsp; - Poly(A) based selection
* [[BioMicroCenter:RNA_LIB#Clontech_SMARTer_Stranded_Total_RNA-Seq_Kit_-_Pico_Input_Mammalian_--_aka_Clontech_ZapR | Clontech ZapR]] <br> &nbsp;&nbsp; - rRNA depletion based
''Small RNA upon request''
 |-
 | style="text-align: center; height: 2em;" | HIGH THROUGHPUT
 | style="text-align: top-left; height: 2em;" | '''Ligation-mediated PCR Methods'''
* NEB Ultra II
'''Tagmentation Methods'''
* Nextera Flex
* Nextera XT
'''Amplicon Methods'''
* 16S Metagenomic
* Custom Amplicon
 | style="text-align: left; height: 2em;" | '''Standard Input'''
* NEB Ultra II Directional RNA with: <br> &nbsp;&nbsp; Poly(A) mRNA Isolation <br> &nbsp;&nbsp; rRNA Depletion (H/M/R)
* 3' Digital Gene Expression (3'DGE)
'''Low Input'''
* Clontech SMART-Seq v2 <br> &nbsp;&nbsp; - Poly(A) based selection
* Clontech ZapR <br> &nbsp;&nbsp; - rRNA depletion based selection
|}

== OTHER SERVICES ==
=== SIZE SELECTION ===
The BMC offers [[BioMicroCenter:PippinPrep|PippinPrep Size Selection]] post library construction if custom insert sizes are needed.
=== QUALITY CONTROL ===
All NGS libraries are quality controlled prior to use. The BioMicro Center offers two 'flavors' of quality control for Illumina libraries.<BR><BR>
Standard quality control includes analysis on the [[BioMicroCenter:QC#AATI_FRAGMENT_ANALYZER|Fragment Analyzer]] and quantification by four point [[BioMicroCenter:RTPCR|qPCR]] quantification for each sample. This confirms both the sizing as well as the presence of Illumina adapters on the libraries.<BR><BR>
Rapid Quality Control is less expensive and quicker. It includes analysis on the [[BioMicroCenter:QC#AATI_FRAGMENT_ANALYZER|Fragment Analyzer]] and quantification by PicoGreen on the [[BioMicroCenter:Varioskan|Varioskan]] for each sample. Final pools are quantified by [[BioMicroCenter:RTPCR|qPCR]] before loading. While this method is significantly faster and less expensive, we cannot guarantee pool balance with it.