Editing BioMicroCenter:2100BioAnalyzer

{{BioMicroCenter}}

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The Agilent 2100 BioAnalyzer is a nanofluidics device that preforms size fractionation and quantification of small samples of DNA, RNA, or Protein. In addition, the BioAnalyzer is able to preform flow cytometry on small numbers of cells. Samples can be run on the BioAnalyzer by individual scientists.

More information about the BioAnalyzer, including examples of the data that we generate in the BioMicro Center can be found on the '''[[BioMicroCenter:BioAnalyzer_INFO | BioAnalyzer Information]]''' page.

== BioMicro Center Services ==
Samples may be run on the BioAnalyzer by individual scientists as supervised users following a one hour training session by [[BioMicroCenter:People | one of our staff]].

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=== Full Service ===
The BioMicro Center does not currently offer the BioAnalyzer as a service operated by BioMicro Center staff.  To submit DNA and RNA samples for size fractionation and quantification, please see the [[BioMicroCenter:Advanced_analytical_Fragment_analyzer|Fragment Analyzer]] or the [[BioMicroCenter:FemtoPulse|FEMTO Pulse]].

=== Supervised Service ===
Samples can also be run by individual users on the BioAnalyzer after a user has received training by emailing bmcwetlab@mit.edu (Core Labs only please). This service will be discontinued if we find the machine is abused. We do request that you use the chips in the BioMicro Center to help us turn over our inventory (so the kits are always in date). Once you are trained in the use of the BioAnalyzer you are welcome to use the machine off business hours (please use the sign in sheet next to the BioAnalyzer).  <BR><BR>

'''IMPORTANT: BIOMICRO CENTER BUSINESS ALWAYS HAS PRIORITY ON THE BIOANALYZER'''<BR><BR>

[https://mit-ki.ilabsolutions.com/equipment/show/265040/?tab=schedule SCHEDULE WALK UP SERVICE]. All reservations must be confirmed by the BMC before use.

=== [[BioMicroCenter:BioAnalyzer_INFO | Available Analyses]] ===
''' DNA '''
* DNA 1000 Kit: 25bp -  1,000bp, 0.1-50ng/uL     
* DNA 7500 Kit: 100bp -  7,500bp, 0.1-50ng/uL
* DNA 12000 Kit: 100bp - 12,000bp, 0.1-50ng/ul
* High Sensitivity DNA kit: 50bp - 7,000bp, 5-500pg/ul

''' RNA '''
* RNA Nano:  25bp - 5,000bp, 25-500 ng/ul total RNA
* RNA Pico: 25bp - 5,000bp, 0.05-5 ng/ul total RNA
* small RNA:  6bp-150bp, 0.05-2 ng/ul miRNAs

''' PROTEIN '''
* Protein 80         5kD- 80kD          60ng/ul
* Protein 230       14kD-230kD          30ng/ul
* High Sensitivity  10kD-250kD         0.3ng/ul

''' FLOW CYTOMETRY '''

== Software ==
Agilent is now providing software for the analysis of BioAnalyzer data to be used on your own PCs. Users have found it somewhat clunky, but it does give you more freedom. The software is available for download from BioMicro at '''http://bmc-apps.mit.edu/''' or from Agilent at http://www.chem.agilent.com/en-US/products/instruments/lab-on-a-chip/2100bioanalyzer/pages/formpage730.aspx

Software Tips and Tricks
*Click the Peak Description button on the top toolbar to switch between peak height, concentration, size, molarity, and migration time.
*Toggle the X axis to change from time to base pairs.
*Using your own standard ladder with corresponding concentrations will generate calibrations and standard curves.
*Customize your own color-coded flags.
*Changing parameters on Globing Scaling will set the same scaling standards for each sample per run.