Editing BioMicroCenter:PricingFY2025 (section)

== LIBRARY GENERATION ==
=== SHORT READ LIBRARY - CONVERSION ===
{| class="wikitable" border=1
 !width=100| Input library
 !width=100| Convert To
 !width=100| Method
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 ! Notes
 |-
 |align="center"|Illumina
 |align="center"|Singular
 |align="center"|Index Overwrite (per sample)
 |align="right"| $30
 |align="right"| $33
 |align="right"| $42
 | Includes QC and reindexing
 |-
 |align="center"|Illumina
 |align="center"|Singular
 |align="center"|P5/P7 conversion (per pool)
 |align="right"| $100
 |align="right"| $110
 |align="right"| $140
 | Includes QC. Samples not reindexed <BR><B> Requires full flowcell for sequencing (Rapid) </B>
 |-
 |align="center"|Illumina
 |align="center"|Element
 |align="center"|Index Overwrite (per sample)
 |align="right"| $30
 |align="right"| $33
 |align="right"| $42
 | Includes QC and reindexing - "Elevate" workflow
 |-
 |align="center"|Illumina
 |align="center"|Element
 |align="center"|P5/P7 splicing (per pool)
 |align="right"| $100
 |align="right"| $110
 |align="right"| $140
 | Includes QC. Samples not reindexed. Requires high input amounts (>10nM)
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable" border=1
 !width=160| ILLUMINA Sample Preparation
 !width=60| Type
 !width=60| Batch Size
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 ! Notes
 |-
 ! colspan="4" | '''STANDARD THROUGHPUT DNA''' <BR> Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
 ! colspan="3" style="color: blue;" | '''HIGH THROUGHPUT DNA''' <BR> Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
 |-
 |rowspan=3 |LIGATION BASED<BR>NEB UltraII
 |align="center"|STD
 |align="center"| per sample
 |align="right"| $105
 |align="right"| $115
 |align="right"| $157
 | Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
 |- style="color: blue;"
 |align="center" rowspan=2|HTL
 |align="center"| 24
 |align="right"| $900
 |align="right"| $990
 |align="right"| $1,260
 |rowspan=2 | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
 |- style="color: blue;"
 |align="center"| 96
 |align="right"| $1,800
 |align="right"| $1,980
 |align="right"| $2,520
 |-

 |Tagmentation <BR> NEXTERA-XT
 |align="center" rowspan=2|STD
 |align="center" rowspan=2|per Sample
 |align="right"| $105
 |align="right"| $115
 |align="right"| $157
 | rowspan="2" | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA). 
 |-
 |Tagmentation <BR> NEXTERA-FLEX
 |align="right"| $157
 |align="right"| $173
 |align="right"| $236
 
 |- style="color: blue;"
 |rowspan=2 | NEXTERA-XT OR FLEX
 |rowspan=2 align="center"| HTL
 |align="center"| ''24''
 |align="right"| $900
 |align="right"| $990
 |align="right"| $1,260
 |rowspan=2 | Includes tagmentation, indexing, enrichment, and quality control spotcheck(SYBR and FA).
 |-
 |align="center" style="color: blue;" | ''96''
 |align="right" style="color: blue;" | $1,800
 |align="right" style="color: blue;" | $1,980
 |align="right" style="color: blue;" | $2,520
 
 |-style="color: blue;"
 |rowspan="2"  | 16S Amplicon
 |align="center" rowspan=2 | HTL
 |align="center"|48
 |align="right" | $1,900
 |align="right" | $2,090
 |align="right" | $2,660
 |rowspan="2"   | Includes normalization, enrichment, pooling and quality control (SYBR and FA). 
 |-style="color: blue;"
 |align="center"|add'l 48
 |align="right" | $650
 |align="right" | $715
 |align="right" | $910

 |-style="color: blue;"
 |rowspan="2"   | Other Amplicon
 |rowspan=2 align="center"| HTL
 |align="center" |48
 |align="right" | $1,600
 |align="right" | $1,760
 |align="right" | $2,240
 |rowspan="2"   | Includes normalization, enrichment, pooling and quality control (SYBR and FA). <BR> Samples *must* be submitted amplified with appropriate linkers.
 |- style="color: blue;" 
 |align="center"|add'l 48
 |align="right" | $630
 |align="right" | $693
 |align="right" | $945
 |-
 ! colspan="7" | '''ADDITIONAL FEES''' <BR> Samples not in proper input format will be subject to these additional charges.
 |-
 |style="color: blue;" colspan=2| High Throughput Setup
 |align="center" style="color: blue;" |48 samples 
 |align="right" style="color: blue;" | $60
 |align="right" style="color: blue;" | $66
 |align="right" style="color: blue;" | $84
 | 
 |-
 |colspan=2|Sample Cleanup
 |align="center"|per sample
 |align="right"| $8
 |align="right"| $8.80
 |align="right"| $11.20
 |SPRI cleanup of samples to remove impurities. 
 |-
 |style="color: blue;" colspan=2|Quantification
 |align="center" style="color: blue;" |per sample
 |align="right" style="color: blue;" | $10
 |align="right" style="color: blue;" | $11
 |align="right" style="color: blue;" | $14
 |Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable" border=1
 !width=160| ILLUMINA Sample Preparation
 !width=60| Type
 !width=60| Batch Size
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 ! Notes
 |-
 ! colspan="4" | '''STANDARD RNA''' <BR> Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
 ! colspan="3" style="color: blue;" | '''HIGH THROUGHPUT RNA''' <BR> Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
 |-
 |rowspan="3"|polyA based isolation <BR><BR> NEB UltraII RNAseq
 |align="center"|<B>STD</B>
 |align="center"| per sample
 |align="right"| $157
 |align="right"| $173
 |align="right"| $236
 |Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
 |-
 |rowspan=2 align="center" style="color: blue;"| <B>HTL</b><BR>(NEB only)
 |align="center" style="color: blue;" | ''24''
 |align="right" style="color: blue;" | $1,500
 |align="right" style="color: blue;" | $1,650
 |align="right" style="color: blue;" | $2,100
 |Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
 |-
 |align="center" style="color: blue;" | ''96''
 |align="right" style="color: blue;" | $3,000
 |align="right" style="color: blue;" | $3,300
 |align="right" style="color: blue;" | $4,200
 |Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
 |-
 
 |rowspan="2"| High Throughput 3' Digital Gene Expression (HT3DGE)
 |rowspan=2  align="center" style="color: blue;"| <B>HTL</b>
 |align="center" style="color: blue;"| ''24'' 
 |align="right" style="color: blue;" | $1,050
 |align="right" style="color: blue;" | $1,155
 |align="right" style="color: blue;" | $1,470
 | rowspan="2"  style="color: blue;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
 |-
 |align="center" style="color: blue;" | ''96''
 |align="right" style="color: blue;" | $2,100
 |align="right" style="color: blue;" | $2,310
 |align="right" style="color: blue;" | $2,940
 |-
 
 |rowspan="3"|Total RNA sequencing <BR><BR> NEB RNAseq +Ribodepletion
 |align="center"|<B>STD</B>
 |align="center"| per sample
 |align="right"| $235
 |align="right"| $258.5
 |align="right"| $352.5
 |Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control. 
 |-
 |rowspan=2 align="center" style="color: blue;"| <B>HTL</b><BR>(NEB only, covaris RNA fragmentation)
 |align="center" style="color: blue;" | ''24''
 |align="right" style="color: blue;" | $2,500
 |align="right" style="color: blue;" | $2,750
 |align="right" style="color: blue;" | $3,500
 |rowspan=2|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
 |-
 |align="center" style="color: blue;" | ''96''
 |align="right" style="color: blue;" | $5,000
 |align="right" style="color: blue;" | $5,500
 |align="right" style="color: blue;" | $7,000
 |-
 
 |rowspan=3|Low Input polyA+ <BR> Clontech SMARTseq + NexteraXT or Flex
 |align="center"| '''STD'''<BR>v4
 |align="center"|per sample
 |align="right"| $262
 |align="right"| $289
 |align="right"| $394
 | rowspan="3" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
 |-
 |rowspan="2" align="center" style="color: blue;"| '''HTL'''<br>v2
 |align="center" style="color: blue;" | ''24''
 |align="right" style="color: blue;" | $2,000
 |align="right" style="color: blue;" | $2,200
 |align="right" style="color: blue;" | $2,800
 |-
 |align="center" style="color: blue;" | ''96''
 |align="right" style="color: blue;" | $3,200
 |align="right" style="color: blue;" | $3,520
 |align="right" style="color: blue;" | $4,480
 |-
 
 |rowspan="3"|Low Input Mammalian Total <BR> Clontech ZapR Kit
 |align="center"| STD
 |align="center"| per sample
 |align="right"| $210
 |align="right"| $231
 |align="right"| $315
 | rowspan="1" | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA). 
 |-
 |rowspan=2 align="center" style="color: blue;"| <B>HTL</b><BR>(covaris RNA fragmentation)
 |align="center" style="color: blue;" | ''24''
 |align="right" style="color: blue;" | $3000
 |align="right" style="color: blue;" | $3300
 |align="right" style="color: blue;" | $4200
 |rowspan=2|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
 |-
 |align="center" style="color: blue;" | ''96''
 |align="right" style="color: blue;" | $5000
 |align="right" style="color: blue;" | $5500
 |align="right" style="color: blue;" | $7000
 |-
 | Small RNA
 |align="center"| STD
 | align="center"|per sample
 | align="right"|$289
 | align="right"|$318
 | align="right"|$433
 | Uses NEB, BIOO or QIAGEN small RNA kit
 |-
 ! colspan="7" | '''ADDITIONAL FEES''' <BR> Samples not in proper input format will be subject to these additional charges.
 |-
 |colspan=2 style="color: blue;" | High Throughput Setup
 |align="center" style="color: blue;" |48 samples 
 |align="right" style="color: blue;" | $60
 |align="right" style="color: blue;" | $66
 |align="right" style="color: blue;" | $84
 | 
 |-
 |colspan=2|Sample Cleanup
 |align="center"|per sample
 |align="right"| $8
 |align="right"| $8.80
 |align="right"| $11.20
 |SPRI cleanup of samples to remove impurities. 
 |-
 |colspan=2 style="color: blue;" |Quantification
 |align="center" style="color: blue;" |per sample
 |align="right" style="color: blue;" | $10
 |align="right" style="color: blue;" | $11
 |align="right" style="color: blue;" | $14
 |Quantification of samples (FA) prior to arraying.
 |-
|}

.

=== LONG READ LIBRARIES ===
{| class="wikitable" border=1
 !Oxford Nanopore Sample Preparation
 !width=120| Nanopore Kit
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 ! Notes
 |-
 |Standard Ligation || LSK || $160 || $176 || $224 
 |rowspan=6|Oxford Nanopore prep methods remain highly experimental and are continually in flux. <BR> As such, we can only estimate cost based on reagents/effort and cannot guarantee results.<BR> Please consult the core prior to sample submission for the latest information.
 |-
 |Native Barcoding <br> (ligation indexing) || - || $50 || $55 || $70
 |-
 |SMARTseq -> Ligation || Cv4+LSK || $300 || $330 || $420
 |-
 |Rapid Prep (tagment - ~10-20kb) || RAD || $150 || $165 || $210
 |-
 |Long Insert Prep || RAD (modified, target >100kb) || $262 || $289 || $394 
 |-
 |Direct RNA || RNA004 || $160 || $176 || $225 
|}