Editing BioMicroCenter:PricingFY2019 (section)

== LIBRARY GENERATION ==
=== ILLUMINA LIBRARY - DNA ===

{| class="wikitable" border=1
 ! ILLUMINA Sample Preparation
 !width=120| Batch Size
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 ! Notes
 |-
 ! colspan="3" | '''STANDARD THROUGHPUT DNA''' <BR> Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
 ! colspan="3" style="color: blue;" | '''HIGH THROUGHPUT DNA''' <BR> Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
 |-
 |rowspan=3 |Standard Illumina Library (LM-PCR) <BR>NEB UltraII
 |align="center"| per sample
 |align="right"| $100
 |align="right"| $110
 |align="right"| $150
 | Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
 |- style="color: blue;"
 |align="center"| 24 samples
 |align="right"| $750
 |align="right"| $825
 |align="right"| $1075
 |rowspan=2 | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
 |- style="color: blue;"
 |align="center"| 96  samples
 |align="right"| $1500
 |align="right"| $1650
 |align="right"| $2250
 |-
 | rowspan="4" | Tagmentation (NEXTERA-XT) - 1ng input
 |align="center"| ''1'' Sample
 |align="right"| $100
 |align="right"| $110
 |align="right"| $150
 | rowspan="4" | Includes tagmentation, indexing, enrichment, and quality control (SYBR and FA). 
 |- style="color: blue;"
 |align="center"| ''16'' <BR>(2 column)<BR> TECAN
 |align="right" | $1000
 |align="right" | $1100
 |align="right" | $1500
 |-
 |align="center" style="color: blue;" | ''96'' <BR> Mosquito
 |align="right" style="color: blue;" | $1500
 |align="right" style="color: blue;" | $1650
 |align="right" style="color: blue;" | $2250
 |-
 |align="center" style="color: blue;" | ''384'' <BR> Mosquito
 |align="right" style="color: blue;" | $3000
 |align="right" style="color: blue;" | $3300
 |align="right" style="color: blue;" | $4500
 |-
 |rowspan="2" style="color: blue;" | 16S Amplicon
 |align="center"|48 samples <BR>(6 columns)
 |align="right" style="color: blue;" | $1,800
 |align="right" style="color: blue;" | $1,980
 |align="right" style="color: blue;" | $2,700
 |rowspan="2" style="color: blue;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA). 
 |-
 |align="center"|add'l 48 samples <BR>(6 columns)
 |align="right" style="color: blue;" | $600
 |align="right" style="color: blue;" | $660
 |align="right" style="color: blue;" | $900
 |-
 |rowspan="2" style="color: blue;" | Other Amplicon
 |align="center" style="color: blue;" |48 samples <BR>(6 columns)
 |align="right" style="color: blue;" | $1,440
 |align="right" style="color: blue;" | $1,584
 |align="right" style="color: blue;" | $2,160
 |rowspan="2" style="color: blue;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA). <BR> Samples *must* be submitted amplified with appropriate linkers.
 |-
 |align="center"|add'l 48 samples <BR>(6 columns)
 |align="right" style="color: blue;" | $600
 |align="right" style="color: blue;" | $660
 |align="right" style="color: blue;" | $900
 |-
 ! colspan="6" | '''ADDITIONAL FEES''' <BR> Samples not in proper input format will be subject to these additional charges.
 |-
 |style="color: blue;" | High Throughput Setup
 |align="center" style="color: blue;" |48 samples 
 |align="right" style="color: blue;" | $50
 |align="right" style="color: blue;" | $55
 |align="right" style="color: blue;" | $75
 | 
 |-
 |Sample Cleanup
 |align="center"|per sample
 |align="right"| $5
 |align="right"| $5.5
 |align="right"| $7.5
 |SPRI cleanup of samples to remove impurities. 
 |-
 |style="color: blue;" |Quantification
 |align="center" style="color: blue;" |per sample
 |align="right" style="color: blue;" | $5
 |align="right" style="color: blue;" | $5.5
 |align="right" style="color: blue;" | $7.5
 |Quantification of samples (FA) prior to arraying.
|}

=== ILLUMINA LIBRARY - RNA ===

{| class="wikitable" border=1
 ! ILLUMINA Sample Preparation
 !width=120| Batch Size
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 ! Notes
 |-
 ! colspan="3" | '''STANDARD RNA''' <BR> Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
 ! colspan="3" style="color: blue;" | '''HIGH THROUGHPUT RNA''' <BR> Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included
 |-
 |rowspan="2" | Standard polyA based isolation <BR> Kapa mRNA Hyperprep
 |align="center"| per sample
 |align="right"| $150
 |align="right"| $165
 |align="right"| $225
 |Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
 |-
 |align="center" style="color: blue;" | ''24'' <br> TECAN
 |align="right" style="color: blue;" | $2000
 |align="right" style="color: blue;" | $2200
 |align="right" style="color: blue;" | $3000
 |Includes polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
 |-
 | rowspan="2"  style="color: blue;" | High Throughput 3' Digital Gene Expression (HT3DGE)
 |align="center" style="color: blue;"| ''24'' <br> TECAN
 |align="right" style="color: blue;" | $1000
 |align="right" style="color: blue;" | $1100
 |align="right" style="color: blue;" | $1500
 | rowspan="2"  style="color: blue;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
 |-
 |align="center" style="color: blue;" | ''96'' <br> TECAN
 |align="right" style="color: blue;" | $4000
 |align="right" style="color: blue;" | $4400
 |align="right" style="color: blue;" | $6000
 |-
 |Total RNA sequencing:Mammalian <BR> Kapa Hyperprep + RiboErase
 |align="center"| per sample
 |align="right"| $225
 |align="right"| $247.5
 |align="right"| $337.5
 |rowspan=2| Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control. 
 |-
 |Total RNA sequencing:Other <BR> Kapa mRNA Hyperprep + Illumina RiboZero
 |align="center"| per sample
 |align="right"| $250
 |align="right"| $275
 |align="right"| $375
 |-
 |Low Input polyA+ <BR> Clontech SMARTseq v4 + NexteraXT
 |align="center"| ''1'' sample
 |align="right"| $250
 |align="right"| $275
 |align="right"| $375
 | rowspan="3" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
 |-
 |rowspan="2"| Low Input polyA+ <BR> SMARTseq v2 + NexteraXT
 |align="center" style="color: blue;" | ''24'' <BR> Mosquito
 |align="right" style="color: blue;" | $1600
 |align="right" style="color: blue;" | $1760
 |align="right" style="color: blue;" | $2400
 |-
 |align="center" style="color: blue;" | ''96'' <BR> Mosquito
 |align="right" style="color: blue;" | $2500
 |align="right" style="color: blue;" | $2750
 |align="right" style="color: blue;" | $3750
 |-
 |Low Input Mammalian Total <BR> Clontech ZapR Kit
 |align="center"| ''1'' sample
 |align="right"| $200
 |align="right"| $220
 |align="right"| $300
 | rowspan="1" | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA). 
 |-
 | Tight Size Cut
 |align="center"| per pool
 |align="right"| $85
 |align="right"| $94
 |align="right"| $130
 | +/- 40bp using PippinPrep
 |-
 | Small RNA
 | align="center"|per sample
 | align="right"|$275
 | align="right"|$303
 | align="right"|$413
 | Uses NEB, BIOO or QIAGEN small RNA kit
 |-
 ! colspan="6" | '''ADDITIONAL FEES''' <BR> Samples not in proper input format will be subject to these additional charges.
 |-
 |style="color: blue;" | High Throughput Setup
 |align="center" style="color: blue;" |48 samples 
 |align="right" style="color: blue;" | $50
 |align="right" style="color: blue;" | $55
 |align="right" style="color: blue;" | $75
 | 
 |-
 |Sample Cleanup
 |align="center"|per sample
 |align="right"| $5
 |align="right"| $5.5
 |align="right"| $7.5
 |SPRI cleanup of samples to remove impurities. 
 |-
 |style="color: blue;" |Quantification
 |align="center" style="color: blue;" |12 samples
 |align="right" style="color: blue;" | $5
 |align="right" style="color: blue;" | $5.5
 |align="right" style="color: blue;" | $7.5
 |Quantification of samples (FA) prior to arraying.
 |-
|}

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=== PACBIO LIBRARIES ===
{| class="wikitable" border=1
 ! PacBio Sample Preparation
 !width=120| Batch Size
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 ! Notes
 |-
 |rowspan=2|SMRTBell Only<BR> eg:PCR products 
 | Sample 1 || $300 || $330 || $450 
 |rowspan=2| Includes QC, repair and addition of indexed SMRTBells
 |-
 | Samples 2-8 || $150 || $165 || $225
 |-
 |rowspan=2|Genomic Library 
 | Sample 1 || $450 || $495 || $675
 |rowspan=2| Includes Covaris gSPIN fragmentation, Pippin sizing, repair and SMRTbell as above
 |-
 | Sample 2-8 || $225 || $250 || $340
|}