Editing BioMicroCenter:Caliper LabChip GX (section)

===How to Read Caliper RNA Analysis Result ===

The Caliper RNA analysis results are almost the same as Agilent Bioanalyzer results. Caliper Life Sciences LabChip GX system analyzes RNA by electrophoretic separation on our microfluidic sipper chips. RNA molecules are separated and subsequently detected via laser induced fluorescence. The LabChip GX Software displays the raw data as an electropherogram and generates a gel-like image for visualization, data such as peak heights, peak areas, concentration, etc., are then calculated and stored as digitized data as text files and tables. These parameters are next used to determine an RQS (RNA Quality Score) number, a calculated score that rates the quality of RNA samples.

•	Caliper RNA Ladder Result (Electropherogram of a typical RNA ladder): Total 9 peaks. The first peak is lower marker used to align the ladder data with data from the sample wells. Others represent different sizing ( 200nt, 500nt, 1000nt, 1500nt, 2000nt, 3000nt, 4000nt and 6000nt).

[[Image:BioMicroCenter_caliper ladder3.jpg|center|200px]]

•	Caliper RNA Eukaryote Sample Result
The electropherogram for typical total RNA samples is shown below. Your results may vary depending on the type of total RNA.

[[Image:BioMicroCenter_caliper RNA2.jpg|center|200px]]

•	Caliper RNA Prokaryot Sample Result
The electropherogram for typical Prokaryote Total RNA samples is shown below. Your results may vary depending on the type of total RNA.

[[Image:BioMicroCenter_caliper RNA3.jpg|center|200px]]

•	RNA mRNA Sample Result
The electropherogram for a typical mRNA sample is shown below. Your results may vary depending on the type and concentration of mRNA.

[[Image:BioMicroCenter_caliper mRNA.jpg|center|200px]]