Editing BioMicroCenter:RTPCR for Solexa (section)

== Plate Preparation: ==
 

-Add 5uL of samples and standards to appropriate wells

-Add 5uL of EB to wells H11 and H12 for negative controls 

-Add 15uL of Master Mix 

<span style="color: red">We have found that the most effective way to load the plate is:
 
-prepare the samples and incomplete master mix (containing everything but SYBR green) 

-plate all of the samples and standards 

-then add SYBR green to master mix and add now complete master mix to all wells being used. 

We have also found that it is beneficial to “cold load” the plate by preparing it over ice.</span> 

'''Final plate set up:'''