Editing BioMicroCenter:RTPCR Protocol for Sample QC (section)

== Preparing Master Mix ==
 

Make the following reaction mix for each well being used.  Keep in mind that each sample requires three wells (triplicates) and the 16 standard wells. 

'''For 1 well: Roche SYBR'''

* Water – 3uL

* PCR Primer Forward (P5), 10uM – 1uL

* PCR Primer Reverse (P7), 10uM – 1uL

* Roche SYBR Green – 10uL 


'''For 1 well: KAPA SYBR'''

* Water – 7.2uL

* PCR Primer Forward (P5), 10uM – 0.4uL

* PCR Primer Reverse (P7), 10uM – 0.4uL

* KAPA Sybr Fast – 10uL

'''<span style="color: purple">Do not add SYBR Green to master mix until right before use. Flick and spin down master mix after SYBR green has been added</span>
'''

<span style="color: red">Master mix may vary depending on type of RT-PCR machine and brand of SYBR green being used</span>