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== SEQUENCING - LOW OUTPUT == Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason. <BR><BR> === [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] === {| class="wikitable" border=1 ! SINGULAR G4 SEQUENCING !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]] !width=80| MIT !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !width=80| unit ! Notes |- |50 PE: F3 Lane (~100m reads) |align="right"| $300 |align="right"| $330 |align="right"| $420 |align="center" | per lane |rowspan=3| Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year |- |Rapid 100nt: F3 flowcell (~400m reads)* |align="right"| $1200 |align="right"| $1321 |align="right"| $1680 |align="center"| per flowcell |- |150 PE: F3 Lane (~100m reads) |align="right"| $450 |align="right"| $495 |align="right"| $630 |align="center"| per lane |} * Rapid flowcells are required for adapted Illumina libraries. === MISEQ SEQUENCING === {| class="wikitable" border=1 ! MISEQ SEQUENCING !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]] !width=80| MIT !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !width=80| unit ! Notes |- |70nt V2 kit <BR> (eg. 30PE or 70SE) |align="right"| $1,320 |align="right"| $1,452 |align="right"| $1,848 |align="center" rowspan=6| per lane |rowspan=6| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year |- |150nt V3 kits <BR> (eg. 75PE) |align="right"| $1,430 |align="right"| $1,573 |align="right"| $2,002 |- |300nt V2 kit<BR> (eg. 150PE) |align="right"| $1,595 |align="right"| $1,754.50 |align="right"| $2,233 |- |500nt V2 kit <BR> (eg. 250PE) |align="right"| $1,760 |align="right"| $1,936 |align="right"| $2,464 |- |600nt V3 kit<BR> (eg. 300PE) |align="right"| $2,200 |align="right"| $2,420 |align="right"| $3,080 |- |500nt '''NANO''' Run <BR> (eg. 250PE) |align="right"| $770 |align="right"| $847 |align="right"| $1,078 |} === OTHER SHORT READ SERVICES === {| class="wikitable" border=1 ! SHORT READ SUPPORTING SERVICES !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]] !width=80| MIT !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !width=80| unit ! Notes |- | Quality Control Only -only one QC is included per sequencing lane. |align="right"| $35 |align="right"| $38.50 |align="right"| $49 |align="center"| per sample | Includes RT-PCR and Frag.Analyzer |- | Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. |align="right"| $15 |align="right"| $16.50 |align="right"| $21 |align="center"| per sample | Includes Fluorometric quantification and Frag.Analyzer |- |qPCR only (short read) |align="right"| $25 |align="right"| $27.50 |align="right"| $35 |align="center"| per sample | Includes qPCR values from standard QC only |- |qPCR only - full plate(short read) |align="right"| $400 |align="right"| $440 |align="right"| $560 |align="center"| per sample | excludes column 1 (used for controls) |}
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