Editing BioMicroCenter:Singular Sequencing (section)

=== Illumina Library to Singular Library Conversions and Custom Sequencing ===
The BioMicro Center can modify Illumina ready libraries by using the p5 and p7 flow cell binding sequences to attach Singular S1 and S2 flow cell binding sequences.  However, this process leaves behind a 16 bp portion of the p5/p7 sequences requiring a different set of primers that will compete with the onboard S1 and S2 index primers.  Like custom runs, these requests are rare and must be run on an entire flow cell.
{|
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  !Service 
  !Singular Sequencing
  |-
  |INPUT || Illumina or Singular libraries
  |-
  |MIN VOLUME || 12 uL
  |-
  |MIN CONCENTRATION || 4 nM
  |-
  |INCLUDED SERVICES 
  | 
* Quality Control:Fragment Analyzer and qPCR
* Singular Sequencing 
* Demultiplexing
  |-
  |ADDITIONAL SERVICES ||
* Quality Control
* Singular library preperation
  |-
  |DATA FORMATS 
  |
*FASTQ (stored 1 year)
*BCL (stored 30d)
  |-
  |QUALITY CONTROL 
  |
* [https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ FASTQC]
* Basic run metrics (alignment rate, complexity)
* Basic RNAseq metrics (where applicable)
* Basic paired end metrics (where applicable)
* Contamination checks
  |-
  |PRICING || [[BioMicroCenter:Pricing#SINGULAR_G4|LINK]]
  |-
  |SUBMISSION 
  |
* MIT - [https://mit.ilabsolutions.com/service_item/new/3381?spt_id=3863 ilabs] 
  |-
 |}