Editing BioMicroCenter:SPRI-Works (section)

=== Sample Submission ===

We suggest that you submit as much volume as possible since the first step of the protocol brings all sample volumes up to 100uL in water.  For optimal yield, libraries should be fragmented so that the average fragment size is approximately 60nt shorter than the chosen size range to allow for the addition of partial adapters.

All samples submitted for '''BMC Fragmented DNA sample prep (SPRI-only)''' must be submitted with '''user supplied''' adapter mix. Adapter mixes should be pre-diluted and should have enough volume for 10uL of adapter per sample. For recommended dilutions for various sample concentrations, please see the chart below:

[[Image:Spri-adaptrecomend.jpg|center|500px]]


Please note that SPRI-only samples will need to be re-submitted separately if you wish to sequence with us later on.

'''[[BioMicroCenter:Forms#Downloads|Sample Submission Forms]]'''