Jump to content
Main menu
Main menu
move to sidebar
hide
Navigation
Main page
Recent changes
Random page
Help about MediaWiki
BioMicro Center
Search
Search
Appearance
Create account
Log in
Personal tools
Create account
Log in
Pages for logged out editors
learn more
Contributions
Talk
Editing
BioMicroCenter:RTPCR for Solexa
(section)
Page
Discussion
English
Read
Edit
View history
Tools
Tools
move to sidebar
hide
Actions
Read
Edit
View history
General
What links here
Related changes
Special pages
Page information
Appearance
move to sidebar
hide
Warning:
You are not logged in. Your IP address will be publicly visible if you make any edits. If you
log in
or
create an account
, your edits will be attributed to your username, along with other benefits.
Anti-spam check. Do
not
fill this in!
== Preparing Master Mix: == Make the following reaction mix for each well being used. Keep in mind that each sample requires three wells (triplicates) and the 16 standard wells. '''For 1 well:''' Water β 3uL PCR Primer Forward (P5), 10uM β 1uL PCR Primer Reverse (P7), 10uM β 1uL Roche SYBR Green β 10uL '''For 45 wells:''' Water β 135uL PCR Primer Forward (P5), 10uM β 45uL PCR Primer Reverse (P7), 10uM β 45uL Roche SYBR Green β 450uL - flick and spin down master mix after SYBR green has been added '''Do not add SYBR Green to master mix until right before use ''' <span style="color: red">Master mix may vary depending on type of RT-PCR machine and brand of SYBR green being used</span>
Summary:
Please note that all contributions to BioMicro Center may be edited, altered, or removed by other contributors. If you do not want your writing to be edited mercilessly, then do not submit it here.
You are also promising us that you wrote this yourself, or copied it from a public domain or similar free resource (see
BioMicro Center:Copyrights
for details).
Do not submit copyrighted work without permission!
Cancel
Editing help
(opens in new window)
