Editing BioMicroCenter:PacBio (section)

=== [http://www.pacb.com/applications/target/index.html AMPLICON / RESEQUENCING] ===
PacBio reads can also be used to looks for variants in specific regions. The long reads allow for better detection of large rearrangements and understanding repetitive regions of the genome. <BR><BR>
The use of long reads in assembly can also establish phasing of mutations. Short reads will typically not be able to span multiple mutations on a single read. PacBio reads are long enough to enable detection of multiple mutations as coming from the same strand. <BR><BR>
Indexing is possible with resequencing amplicons but indexes should be added during PCR and not during library preparation.