Editing BioMicroCenter:HTLR TEST HTLR TEST (section)

== Cv2 ==
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  ! style="text-align: center; width: 150px;" | Parameter
  ! style="text-align: center; width: 300px;" | General requirements
  ! style="text-align: center; width: 300px;" | [[BioMicroCenter:HTLR_TEST_HTLR_TEST#RNA_Pre-load_Submissions|Pre-load requirements]]
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  | style="text-align: center; height: 2em;" | SAMPLE INPUT
  | colspan="2" style="text-align: center; height: 2em;" | Single cells or [[BioMicroCenter:RIN|intact]] low RNA input
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  | style="text-align: center; height: 2em;" | RANGE OF INPUT
  | style="text-align: center; height: 2em;" | 10pg - 250pg<br>(Or as available for single cells)
  | style="text-align: center; height: 2em;" | Single cells pre-arrayed across plate or [[BioMicroCenter:HTLR_TEST_HTLR_TEST#Normalization|normalized]] RNA
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  | style="text-align: center; height: 2em;" | SUBMISSION VOLUME
  | style="text-align: center; height: 2em;" | &ge;5&micro;L nuclease free water
  | style="text-align: center; height: 2em;" | Single cell or RNA in 1&micro;L nuclease free water
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  | style="text-align: center; height: 2em;" | UNIT
  | colspan="2" style="text-align: center; height: 2em;" | 24 samples <BR> 96 samples
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  | style="text-align: center; height: 2em;" | PLATE SETUP
  | style="text-align: center; height: 2em;" | Samples should be arrayed by column in a 96-well full-skirt plate (Axygen)
  | style="text-align: center; height: 2em;" | Samples arrayed by column in [[BioMicroCenter:HTLR_TEST_HTLR_TEST#Quadrant_Layout|1st quadrant]] of 384-well hard-shell plate (BioRad)
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  | style="text-align: center; height: 2em;" | SEQUENCING RECOMMENDATIONS
  | colspan="2" style="text-align: center; height: 2em;" | All platforms
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  | style="text-align: center; height: 2em;" | INDEX AVAILABILITY
  | colspan="2" style="text-align: center; height: 2em;" | Up to 384 [[BioMicroCenter:HTLR_TEST_HTLR_TEST#Unique_versus_Combinatorial_Dual_Indexing|Unique Dual Indexes]] (w/ Nextera Flex) <br> 384 [[BioMicroCenter:HTLR_TEST_HTLR_TEST#Unique_versus_Combinatorial_Dual_Indexing|Combinatorial Dual Indexes]]
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  | style="text-align: center; height: 2em;" | INCLUDED
  | colspan="2" style="text-align: center; height: 2em;" |cDNA generation of poly(A) RNA <br>cDNA amplification<br>Spot check of cDNA<br>Library preparation (Nextera Flex or XT)<br>Spot check of final libraries  
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  | style="text-align: center; height: 2em;" | ADDITIONAL SERVICES AVAILABLE
  | style="text-align: center; height: 2em;" | Sample QC <BR> Sample cleaning <BR> Sample arraying <BR> Sample re-prep
  | style="text-align: center; height: 2em;" | [[BioMicroCenter:HTLR_TEST_HTLR_TEST#RNA_Pre-load_Submissions|N/A]]
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  | style="text-align: center; height: 2em;" | SUBMISSION FORM
  | colspan="2" style="text-align: center; height: 2em;" | MIT - [https://mit.ilabsolutions.com/service_item/new/3381?spt_id=3863 ilabs] <BR> External - [[BioMicroCenter:Forms|form]]
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  | style="text-align: center; height: 2em;" | PRICING
  | colspan="2" style="text-align: center; height: 2em;" | [[BioMicroCenter:Pricing#ILLUMINA_LIBRARY_-_RNA|LINK]]
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:Our Cv2 prep is a "home-brew" version of Clontech's SMART-Seq kits which generates cDNA of full length transcripts from low inputs of RNA (i.e. single cell, dilute RNA sample). This workflow plugs into either our HTL [[BioMicroCenter:HTLD_TEST_HTLD_TEST#Nextera_Flex|Nextera Flex]] or [[BioMicroCenter:HTLD_TEST_HTLD_TEST#Nextera_XT|XT]] preps where the cDNA is tagmented and indexed.<br>
:This method uses oligo-d(T) priming to selectively process polyadenylated RNA which is then amplified by an initial round of PCR, making this prep ideal low-input high quality RNA or single cells. Due to the nature method, submissions should be coordinated with BMC to schedule sample drop-off of up to 384 samples to begin processing immediately in order to reduce possible loss/degradation of sample. <br>
[[Image:Cv2_BMC.png|center|350px]]
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