Editing BioMicroCenter:SPRI-Works (section)

=== Effect of Concentration ===
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|SPRIworks prepared samples have produced high-quality data even when sample input was below the manufacturer's recommendations. To test the dynamic range, we loaded varying quantities (200ng, 50, 12.5, 3.2, 0.8 and 0.2) of 50bp ladder from New England Biolabs on the SPRIworks to produce libraries. We then measured yield using qPCR and the Agilent BioAnalyzer. Results showed a consistent yield between 10% and 30% for the 200bp insert band consistent with the SPRI beads not being limiting.
|[[Image:DNA_prep_3.png|thumb|center|300px|Constructing Illumina libraries from 50bp ladder]] 
|[[Image:DNA_prep_4.png|thumb|center|300px|Yield of library constructions as measured for the 200nt band]]
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|[[Image:BMC_libraryComplexity.png|thumb|center|300px|Relationship between input material for SPRI and library complexity]]
|colspan="2"| Experience with the SPRIworks has enabled us to build up an understanding of the relationship between material submitted for the library and the complexity of the library. To the left is data from RNAseq libraries prepared for Sidi Chen of the Sharp lab. The linear correlation between number of molecules sequenced and the input is clearly visible. High complexity libraries required 1ng of input.
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