Editing BioMicroCenter:RNAseq (section)

=== [http://www.clontech.com/US/Products/cDNA_Synthesis_and_Library_Construction/cDNA_Synthesis_Kits/Ultra_Low_Input_RNA_cDNA_Synthesis Clontech SMARTer Low-Input] ===
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For samples with less then 100ng of input, the BioMicro Center utilizes the [http://www.clontech.com/US/Products/cDNA_Synthesis_and_Library_Construction/cDNA_Synthesis_Kits/Ultra_Low_Input_RNA_cDNA_Synthesis Clontech SMARTer system]. This system differs from the TruSeq chemisry in that it begins with cDNA generation using polyT priming and proprietary chemistry. The use of polyT priming requires the RNA to be of high quality. Full length double-stranded cDNAs are generated and amplified by PCR. These cDNAs are then fragmented and transferred to the [[BioMicroCenter:SPRI-Works|SPRIworks]] for the remainder of sample preparation. Data from this system is of similar quality to samples created with Illumina TruSeq chemistry.
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[[Image:BMC_ClontechChemistry.png|thumb|right|200px|Clontech system.<BR>Image from Clonetech]]
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