Editing BioMicroCenter:RNA HTL (section)

=== NEB Ultra II Directional RNA with rRNA Depletion ===
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  ! style="text-align: center; width: 150px;" | Parameter
  ! style="text-align: center; width: 300px;" | General requirements
  ! style="text-align: center; width: 300px;" | [[BioMicroCenter:RNA_HTL#RNA_Pre-load_Submissions|Pre-load requirements]]
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  | style="text-align: center; height: 2em;" | SAMPLE INPUT
  | colspan="2" style="text-align: center; height: 2em;" | [[BioMicroCenter:RIN|Intact]] or [[BioMicroCenter:RIN|degraded]] - '''Human/Mouse/Rat samples only!'''<br>''(Bacterial in development)''
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  | style="text-align: center; height: 2em;" | RANGE OF INPUT
  | style="text-align: center; height: 2em;" | 10ng - 100ng
  | style="text-align: center; height: 2em;" | [[BioMicroCenter:RNA_HTL#Normalization|Normalized]] sample
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  | style="text-align: center; height: 2em;" | SUBMISSION VOLUME
  | style="text-align: center; height: 2em;" | >10&micro;L nuclease free water
  | style="text-align: center; height: 2em;" | Exactly 5&micro;L nuclease free water (day of submission)
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  | style="text-align: center; height: 2em;" | UNIT
  | colspan="2" style="text-align: center; height: 2em;" | 24 samples <BR> 96 samples
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  | style="text-align: center; height: 2em;" | PLATE SETUP
  | style="text-align: center; height: 2em;" | Samples should be arrayed by column in a 96-well full-skirt plate (Axygen)
  | style="text-align: center; height: 2em;" | Samples arrayed by column in [[BioMicroCenter:RNA_HTL#Quadrant_Layout|1st quadrant]] of 384-well hard-shell plate (BioRad)
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  | style="text-align: center; height: 2em;" | SEQUENCING RECOMMENDATIONS
  | colspan="2" style="text-align: center; height: 2em;" | All platforms
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  | style="text-align: center; height: 2em;" | INDEX AVAILABILITY
  | colspan="2" style="text-align: center; height: 2em;" |112 [[BioMicroCenter:RNA_HTL#Unique_versus_Combinatorial_Dual_Indexing|Unique Dual Indexes]] <br> 192 [[BioMicroCenter:RNA_HTL#Unique_versus_Combinatorial_Dual_Indexing|Combinatorial Dual Indexes]]
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  | style="text-align: center; height: 2em;" | INCLUDED
  | colspan="2" style="text-align: center; height: 2em;" | RNA selection <br> cDNA synthesis <br> Library preparation <BR> Spot check of final libraries
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  | style="text-align: center; height: 2em;" | ADDITIONAL SERVICES AVAILABLE
  | style="text-align: center; height: 2em;" | Sample QC <BR> Sample cleaning <BR> Sample arraying <BR> Sample re-prep
  | style="text-align: center; height: 2em;" | [[BioMicroCenter:RNA_HTL#RNA_Pre-load_Submissions|N/A]]
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  | style="text-align: center; height: 2em;" | SUBMISSION FORM
  | colspan="2" style="text-align: center; height: 2em;" | MIT - [https://mit.ilabsolutions.com/service_item/new/3381?spt_id=3863 ilabs] <BR> External - [[BioMicroCenter:Forms|form]]
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  | style="text-align: center; height: 2em;" | PRICING
  | colspan="2" style="text-align: center; height: 2em;" | [[BioMicroCenter:Pricing#ILLUMINA_LIBRARY_-_RNA|LINK]]
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:NEBNext Ultra II Directional RNA preparation with ribosomal RNA (rRNA) depletion is used to deplete rRNA by enzymatic degradation using single-stranded DNA probes that target rRNAs, leaving all other RNA species present and available for library preparation. This depletion method is agnostic to input sample quality and is the go-to method if samples don't meet quality requirements for poly(A) selection. We have  miniaturized this rRNA depletion method to 1/6th and the following RNA library preparation to 1/10th the original reaction volume on the Mosquito HV. This method is suited for inputs ranging from 5ng to 100ng (recommended input of > 50ng). Due to probe design used in the depletion, this method is currently only available for species where NEB has probes available.
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:[[IMAGE:Depletion_HTL.jpg|350px]] 
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