Editing BioMicroCenter:Sequencing Quality Control (section)

== Other Sample QC techniques not recommended ==

* [[BioMicroCenter:NanoDrop ND-1000|NanoDrop ND-1000]]- The NanoDrop is one of the most commonly used tools to measure the concentration of DNA in solution. The NanoDrop has a detection limit of about 5ng/ul. Unfortunately, due to noise at the lower detection limit, samples speced on the Nanodrop have not shown reliable results on the Solexa sequencer. We do not recommend using the NanoDrop as the primary method of determining concentration for samples on the Sequencer. 

* Qubit - The Qubit is a selective flurometer that uses fluorescent dyes specific for dsDNA, RNA or protein to quantify samples. The Qubit is used in the Fraenkel lab to quantify their samples for Solexa sequencing. Information about this application can be found at: http://www.invitrogen.com/site/us/en/home/brands/Product-Brand/Qubit.html

* PicoGreen - PicoGreen is a fluorescent dye that binds specifically to dsDNA and allows for quantification. PicoGreen can be measured in a few different ways, the Boyer lab uses a photospectrometer and  Invitrogen states that the Qubit can also be used. More information can be found at: http://probes.invitrogen.com/media/pis/mp07581.pdf