Editing BioMicroCenter:RNA LIB (section)

== [http://www.clontech.com/US/Products/cDNA_Synthesis_and_Library_Construction/cDNA_Synthesis_Kits/Ultra_Low_Input_RNA_cDNA_Synthesis Clontech SMARTseq Low-Input] ==
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  !Service 
  !Low input RNA Library Prep
  |-
  |INPUT || Clean eukaryotic total RNA <BR> RIN 9+ <BR> 10pg <BR> >10uL (where possible)
  |-
  |INCLUDED || Initial QC by FemtoPulse <BR> Library preparation <BR> Illumina QC
  |-
  |SUBMISSION || MIT - [https://mit.ilabsolutions.com/service_item/new/3381?spt_id=3863 ilabs] <BR> External - [[BioMicroCenter:Forms|form]]
  |-
  |UNIT || Per sample
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For samples with less then 50ng of input, the BioMicro Center utilizes the [http://www.clontech.com/US/Products/cDNA_Synthesis_and_Library_Construction/cDNA_Synthesis_Kits/Ultra_Low_Input_RNA_cDNA_Synthesis Clontech SMARTseq v4 system]. This system differs from the TruSeq chemistry in that it begins with cDNA generation using polyT priming followed by strand switching oligos. The use of polyT priming requires the RNA to be of high quality. Full length double-stranded cDNAs are generated and amplified by PCR. These cDNAs can be prepared into NGS short-read libraries using the NexteraXT chemistry from Illumina. Data from this system is of similar quality to samples created with Illumina TruSeq chemistry but is not stranded. Single samples can be prepared by hand. [[BioMicroCenter:RNA_HTL|Batches of 24, 96 or 384 samples]] can be prepared using the older SMARTseq v2 chemistry on the Mosquito HV resulting in significantly lower costs/sample.

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