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BioMicroCenter:PricingFY2013
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=== SAMPLE PREPARATION === {| border=1 align="left" ! ILLUMINA Sample Preparation !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]/MIT !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !width=100| unit ! Notes |- | colspan="5" | '''STARTING WITH DNA''' |- | Full Service Sheared DNA (SPRIWORKS) |align="right"| $150 |align="right"| $225 |align="center"| per sample | Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). |- | ''SPRI-TE use only'' |align="right"| $100 |align="right"| N/A |align="center"| per sample | User must provide all primers. We do not QC the samples. Samples will be returned for amplification. |- | Full Service Intact Genomic DNA (NEXTERA) |align="right"| $150 |align="right"| $225 |align="center"| per sample | Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). |- | colspan="5" | '''STARTING WITH RNA''' |- | Illumina TruSeq |align="right"| $300 |align="right"| $450 |align="center"| per sample | Preformed using TruSeq AND SPRIworks system. <br> Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). |- |''+strand specific'' |align="right"| $30 |align="right"| $45 |align="center"| per sample | Requires 1ug of RNA. Uses the dUTP method. |- | Epicenter Ribozero<br>''no polyA pulldown'' |align="right"| $350 |align="right"| $525 |align="center"| per sample | Requires 1ug of RNA. Based on Illumina Truseq chemistry |- | Low Input (Clontech) |align="right"| $375 |align="right"| $575 |align="center"| per sample | Can work on single cells |- | Low Input (NuGen) |align="right"| $400 |align="right"| $600 |align="center"| per sample | Can work on degraded RNA. |- | Tight Size Cut |align="right"| $75 |align="right"| $125 |align="center"| per pool | +/- 40bp using PippinPrep |- | colspan="5" | '''STARTING WITH CHROMATIN''' Note: User must supply antibodies. |- | Full Service ChIP-seq |align="right"| $210 |align="right"| $315 |align="center"| per sample | Preformed using IP-Star AND SPRIworks system. <br> Includes ChIP, library contruction and barcoding. |- | ''IP-Star Only'' |align="right"| $70 |align="right"| $105 |align="center"| per sample | Preformed using IP-Star |- | ''qPCR of ChIP''<br> ''in development'' |align="right"| TBA |align="right"| TBA |align="center"| per sample |Tests 8 IPs against 5 genomic loci in duplicate. User provides oligos. |- |} <BR> <BR> <BR> . <BR> <BR> <BR> <BR> <BR>
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