Editing BioMicroCenter:BioMark (section)

=== WorkFlow ===
* Input
 1ng - 250ng is standard input
 Cell direct Kit from Invitrogen for single cells 
 Use 2-3 lines per sample as replicates.

* Specific target preamplification

    48/96 Asssys mix (@180nM)
    cDNA sample
    preamp master mix

    
    14 (reg)  / 18 (1 cell) cycles of PCR
    
    Dilute 1:5

* prep assays + samples

    2x Master mix   2.50ul / sample - NEEDS ROX - ABI universal master mix
    Loading reagent 0.25ul / sample (detergent to reduce surface tension)
    cDNA            2.25ul pre amp

    20x primer probe 2.5ul (TaqMan)
    Loading Reagent2 2.5ul detergent


* prime chip
* pipette samples + assays
* load / mix chip
* RT-PCR
* Data Analysis

   Plots are heatmaps and RT curves
   similar plots for deltaCT and DDCT