Editing BioMicroCenter:BioAnalyzer INFO (section)

== PROTEIN ==
The same principles for RNA and DNA apply for protein. Here, the model is SDS-PAGE with samples denatured before loading and run in reducing (+DTT) or non-reducing conditions. The standard and high sensitivity assays are moderately different from each other. Standard assays work like DNA and RNA in that the sample is added to a dye and run together in the lane. With the high-sensitivity system, the buffers are exchanged by standard chromatography to tune the pH and remove contaminating chemicals before being labeled. The labeled proteins then are run on the chip - or can be used for additional purification steps before running. BMC is currently [[BioMicroCenter:High Sensitivity Protein Optimization|optimizing the High Sensitivity assay]].

Information from Agilent: [http://www.chem.agilent.com/en-us/products/instruments/lab-on-a-chip/2100bioanalyzer/proteinkits/pages/default.aspx  Protein] - [http://www.chem.agilent.com/en-us/products/instruments/lab-on-a-chip/pages/gp1566.aspx  How it works] ''(Agilent)''

Protein arrays    Protein Weight    Sensitivity
* Protein 80         5kD- 80kD          60ng/ul
* Protein 230       14kD-230kD          30ng/ul
* High Sensitivity  10kD-250kD         0.3ng/ul