Editing BioMicroCenter:Singular Sequencing (section)

===Lane-by-lane Requirements===
*Must be 50 or 150 paired-end dual indexed
*Pool must be compatible with TruSeq, Nextera, TruSeq, smRNA and Solexa sequencing primers

Minimum reads per lane are guaranteed if: 
*the BMC has performed quality control, 
*the samples are high-complexity, especially for the first 10 nt of read 1, and
*submitted libraries are at least 2 nM.

All other requests, including use of custom sequencing primers require a full flowcell. Illumina libraries which have been converted in the Center will also require a full flowcell.

*Min custom primer submission: 20 uL at 100 uM


[[IMAGE:IndexhopF3.jpeg|left|250px|Index hopping for a standard set of Singular libraries]] [[IMAGE:PPPPF3.jpeg|right|200px|Percent Perfect Plot for Singular libraries 150PE]]

Index hopping on Singular runs has been low. The performance of an F3 flowcell mimics other short-read 4 color chemistry sequencers with patterned flowcells. 
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