Editing BioMicroCenter:SPRI-Works (section)

=== Size Selection ===
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|One of the more remarkable aspect of the SPRIbeads is their ability to provide size selection. SPRI beads are supplied in very high salt/PEG buffer that is needed for their binding. At low amounts of salt and PEG only large molecules are bound to the beads. As more salt is added, smaller fragmetns become bound to the DNA. This allows the "Double SPRI" size selection. A low amount of salt+beads is added to the mix and the high molecular weight material is discarded. Then additional beads (and salt) are added and a smaller size cut is taken with the beads while the low molecular weight material is discarded. On the SPRIworks, there are three size range options available, 200-400bp, 300-600bp, or no size selection. (These ranges apply to the inserts together with the partial adapters.)<br>
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