Editing BioMicroCenter:RTPCR for Solexa (section)

== RT-PCR Protocol ==

'''QPCR Quantification for Solexa Sequencing Libraries'''


'''Materials:'''

-Enriched sample libraries (with either paired end or standard adapters)

-Phix 335bp control library (Cat. No. CT-901-1001)

-Qiagen Buffer EB

-Molecular biology grade water

-10uM Forward Primer: AATGATACGGCGACCACCGA

-10uM Reverse Primer: CAAGCAGAAGACGGCATACGA

-LightCycler 480 SYBR Master Mix (Cat. No. 04707516001)

-LightCycler 480 Multiwell Plate 96, Clear (Cat No. 05102413001)

-Light Cycler 480 Sealing Foil (included in Lightcycler plate order)

-Light Cycler 480 II RT-PCR machine (Roche)

<span style="color: red">SYBR Green and 96 well plate listed in materials are specific for Roche LightCycler 480, please adjust for use on other RT-PCR machines</span>

'''Remember to turn on the RT-PCR machine before preparing your plate so it can warm up.
'''