Editing BioMicroCenter:RTPCR (section)

=== Light Cycler 480 II Real-time PCR Machines ===
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  ! Instrument || LC480
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  | TYPE || WALKUP - MIT only
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  | UNIT || Per Plate
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  | FORMAT || 96w/394w
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  | SIGNUP || [https://mit.ilabsolutions.com/equipment/show/261680/?tab=schedule ilabs]
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  | NEW USERS ||  New users should request training by emailing biomicro@mit.edu
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  | DONATED BY || Prof Laurie Boyer, Prof Leonard Guarente, Dept of Biology
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The [https://www.roche-applied-science.com/sis/rtpcr/htc/index.jsp LightCycler 480 Real-Time PCR System] is a fully integrated multiwell-plate based real-time PCR platform for highly accurate qualitative and quantitative detection of nucleic acids. The instrument handles both 96 and 384w plates. The LC480 can measure Ct and melt curves and can do protein melt curves as well. The instrument handles SYBR and Taqman probes. ROC standards are not required on the instrument, though it can detect them. <BR><BR>

There is a charge per plate to cover the cost of the service contract.<BR><BR> 

96 well, 384 well plates and KAPA SYBR Universal Fast Mastermix 2x can also be purchased from the BioMicro Center for use on the LightCycler480<BR><BR> 

'''Please Note:''' There are three LightCycler systems (Thing 1 96w, Thing 2 384w, and Thing 3 384w) that have a sign up <B>maximum of four hours per day</b> during business hours (10AM to 5PM), all time slots are available the night before even if it exceeds the maximum. 

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[[Image:LC480.jpg|right]]
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