Editing BioMicroCenter:QC (section)

== AATI FEMTO PULSE ==
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  !Service 
  !AATI FEMTO PULSE
  |-
  |INPUT || 5uL for standard <br> 2uL/well for preload
  |-
  |SUBMISSION || MIT - [https://mit.ilabsolutions.com/service_item/new/3381?spt_id=4110 ilabs] -- External - [[BioMicroCenter:Forms|form]] <BR> Preloaded rows must be in semiskirted plates from the BioMicro Center (or equivalent).
  |-
  |UNIT || Samples or preloaded rows
  |-
  |TURN AROUND || Typically < 1 business day
  |-
  |DONATED BY || MIT CEHS
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The [[BioMicroCenter:FemtoPulse|FEMTO Pulse (FP)]] is an instrument that provides a remarkable upgrade over the existing FA and Agilent BioAnalyzer2100 technologies that have been available for many years. While still an instrument to analyze the lengths of nucleic acids, the FEMTO Pulse adds a pulse filed generator to allow the separation and characterization of significantly longer molecules, out to 200,000 base pairs. Additionally, the FP has significantly improved optics that allow approximately two orders of magnitude of additional sensitivity. Where the FA bottoms out a sensitivity of 2-3pg/ul, the FP has nearly 100x improved sensitivity which may enable this instrument to directly observe molecular reactions without the need for amplification or modification with radioactive nucleotides. <BR><BR>

The FEMTO Pulse runs 12 samples at a time using 2ul of material. Each run takes ~90 minutes to run. It is *not* a replacement for the Fragment Analyzer and is optimized for very low inputs or very long samples.  <BR><BR>
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[[image:BMC_FP_instrument.jpeg|right]]
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  ! Assay
  ! Sizing Range
  ! Concentration Range
  |-
  |NGS DNA
  |50bp - 5kbp
  |Smear: 2.5-500pg/uL <br> Fragment 50fg-5pg/uL
  |-
  |Genomic DNA
  |2kbp - 160kbp
  |Smear: 2.5 - 500pg/uL
  |-
  |Femto RNA 
  |50bp - 5kbp
  |2.5pg-500pg/uL
  |}
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