Editing BioMicroCenter:PacBio Library Preparation (section)

===Standard Workflow===
The BioMicro Center uses the Express Template Prep Kit v2.0 for dsDNA library preparation. This kit is recommended by PacBio for all libraries to be sequenced with Sequel Chemistry 3.0 for Revio. Single strand overhangs are removed, and DNA Damage repair is performed.  End-Repair and A-tailing proceed before SMRTBell A/T Ligation, after which two rounds of SPRI cleans with PacBio approved beads clean the prepared library.  Prepared libraries can be quantified via Qubit prior to polymerase binding.  If a size selection is necessary, the size will be verified by Fragment Analyzer or by Femto Pulse for sizes larger than 6 kbp.
|[[IMAGE:PacBioBound.jpg|thumb|center|400px|PacBio Library with Enzyme bound to SMRTBell hairpins<br>NOT TO SCALE]]
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