Editing BioMicroCenter:PEM (section)

== Sample Submission Guidelines ==

'''Users are required to prepare their own libraries for sequencing''' by following the corresponding Illumina [[BioMicroCenter:Protocols| Protocols]]. If this is a service that you would like the BMC to perform in the future please visit the [[BioMicroCenter:Ideas|Ideas]] page.  Illumina Paired End sample preparation kits can be purchased directly from Illumina or through the BioMicro Center for $3,600.00 plus shipping costs (10 sample preps per kit). To request an order or for more information please contact [[BioMicroCenter:People|Ali Perrotta]]

Samples which are ready for sequencing should be gel-purified according to your protocol, and provided as at least 5uL of solution at a concentration of at least 10 nM in EB/ Tween 0.1%. To calculate your sample’s molar concentration, you must know the average length of your product. 

When submitting a sample you must supply the BMC with the molar concentration, average length, and species of origin of all samples. 

We encourage all those submitting samples to allow us to run QC to obtain/confirm concentration information. For more information on our QC methods please see the [[BioMicroCenter:Sequencing_Quality_Control|Sequencing Quality Control]] page.

Samples generated using standard kits with Illumina PCR primers can be submitted without sequencing primers. Custom sample preparations using customized PCR primer sequences should be provided along with a complementary sequencing primer.

[[BioMicroCenter:Forms#Downloads|Sample Submission Forms]]