Editing BioMicroCenter:NanoPore Library Prep (section)

== dsDNA: SQK-RBK114 ==
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  !Parameter 
  !Requirements
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  |INPUT || Clean double-stranded DNA <BR> 400 ng+ <BR> >30 kbp <br> >10uL
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  |INCLUDED || Initial QC by UV-vis and FemtoPulse <BR> Library preparation <BR> Final QC by Qubit
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  |SUBMISSION || MIT - [https://mit.ilabsolutions.com/service_item/new/3381?spt_id=3863&tab=services ilabs] <BR> External - [[BioMicroCenter:Forms|form]]
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  |UNIT || Per sample
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  |PRICING ||[[BioMicroCenter:Pricing#LONG_READ_LIBRARIES|LINK]] 
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The BioMicro Center utilizes the SQK-RBK114 kit for tagmentation-based library preparation for dsDNA of large size distributions. Oxford recommends sizes greater than 30 kbp with input of at least 400 ng. The enzymes are bought directly from New England Biolabs. Input is tagmented with adapters and the products are size selected using SPRI beads. Prepared library will be quantified by Qubit prior to loading.
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This kit can also be used when users are interested in obtaining the longest reads possible.  For this protocol, micrograms of clean genomic DNA must be used.  No size selection is performed.  The PromethION P2S has sequenced reads with lengths of 300 kbp+:  however, these long reads are rare outliers and the overall read amount for the flowcell in use will be lowered.  Higher Molecular Weight DNA seems to kill the nanopores faster and requires a large amount of computing power from the sequencer, reducing the real time speed of basecalling and delaying data delivery.
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