Editing BioMicroCenter:Illumina Sequencing (section)

===Typical Workflow===
Illumina sequencing at the core begins with library quality control, during which the Center verifies the anchor elements and insert size using [[BioMicroCenter:RTPCR|qPCR]] and the [[BioMicroCenter:QC#AATI_FRAGMENT_ANALYZER|Fragment Analyzer]]. Users may elect to bypass this step if they provide the sample concentration and the concentration they would like to load at. Samples are then entered into the sequencing queue. Typical queues in the BioMicro Center are short, rarely exceeding 1-2 weeks, and samples are frequently run within a couple days. <B>We do guarantee a minimum number of reads per lane provided if: a) BMC performed the QC, b) the samples are high-complexity, especially in the first few nucleotides, and c) the samples are at least 2nM.</B> <br>

Illumina sequencing through the BioMicro Center is only available in full lanes and not on a per read basis. You are welcome to collaborate with other laboratories in order to share a lane. However, please be sure to minimize any possibility of cross-contamination of indexes as well as [https://www.illumina.com/science/education/minimizing-index-hopping.html index crosstalk.] 

When ordering lanes, please be aware that only full flowcells will be sequenced. More lanes may be purchased to facilitate sequencing. The wait time for individual lanes can vary widely. Full flowcells have priority for sequencing.<BR><BR>