Editing BioMicroCenter:DNA HTL (section)

== FRAGMENTED DNA ==
=== NEB Ultra II ===
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  ! style="text-align: center; width: 150px;" | Parameter
  ! style="text-align: center; width: 300px;" | General requirements
  ! style="text-align: center; width: 300px;" | [[BioMicroCenter:DNA_HTL#DNA_Pre-load_Submissions|Pre-load requirements]]
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  | style="text-align: center; height: 2em;" | SAMPLE INPUT
  | colspan="2" style="text-align: center; height: 2em;" | Fragmented DNA (i.e. ChIP-Seq, small PCR amplicons)
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  | style="text-align: center; height: 2em;" | RANGE OF INPUT
  | style="text-align: center; height: 2em;" | 100pg - 75ng
  | style="text-align: center; height: 2em;" | Samples all [[BioMicroCenter:DNA_HTL#Normalization|normalized]] to same concentration in 10&micro;L (100pg/&micro;L - 7.5ng/&micro;L)
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  | style="text-align: center; height: 2em;" | SUBMISSION VOLUME
  | style="text-align: center; height: 2em;" | >10&micro;L 10 mM Tris pH 8.0
  | style="text-align: center; height: 2em;" | Exactly 10&micro;L 10mM Tris pH 8.0
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  | style="text-align: center; height: 2em;" | UNIT
  | colspan="2" style="text-align: center; height: 2em;" | 24 samples <BR> 96 samples
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  | style="text-align: center; height: 2em;" | PLATE SETUP
  | style="text-align: center; height: 2em;" | Samples should be arrayed by column in a 96-well full-skirt plate (Axygen)
  | style="text-align: center; height: 2em;" | Samples arrayed by column in [[BioMicroCenter:DNA_HTL#Quadrant_Layout|1st quadrant]] of 384-well hard-shell plate (BioRad)
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  | style="text-align: center; height: 2em;" | SEQUENCING RECOMMENDATIONS
  | colspan="2" style="text-align: center; height: 2em;" | All platforms
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  | style="text-align: center; height: 2em;" | INDEX AVAILABILITY
  | colspan="2" style="text-align: center; height: 2em;" |112 [[BioMicroCenter:DNA_HTL#Unique_versus_Combinatorial_Dual_Indexing|Unique Dual Indexes]] <br> 192 [[BioMicroCenter:DNA_HTL#Unique_versus_Combinatorial_Dual_Indexing|Combinatorial Dual Indexes]]
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  | style="text-align: center; height: 2em;" | INCLUDED
  | colspan="2" style="text-align: center; height: 2em;" | Library preparation <BR> Spot check of final libraries
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  | style="text-align: center; height: 2em;" | ADDITIONAL SERVICES AVAILABLE
  | style="text-align: center; height: 2em;" | Sample QC <BR> Sample cleaning <BR> Sample arraying <BR> Sample re-prep
  | style="text-align: center; height: 2em;" | [[BioMicroCenter:DNA_HTL#DNA_Pre-load_Submissions|N/A]]
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  | style="text-align: center; height: 2em;" | SUBMISSION FORM
  | colspan="2" style="text-align: center; height: 2em;" | MIT - [https://mit.ilabsolutions.com/service_item/new/3381?spt_id=3863 ilabs] <BR> External - [[BioMicroCenter:Forms|form]]
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  | style="text-align: center; height: 2em;" | PRICING
  | colspan="2" style="text-align: center; height: 2em;" | [[BioMicroCenter:Pricing#ILLUMINA_LIBRARY_-_DNA|LINK]]
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:NEBNext Ultra II is used for standard ligation-mediated PCR based approaches. We have miniaturized the reaction volume to a 1/5th scale that reliably performs on inputs ranging from 10pg to 75ng (recommended minimum of 100pg). The reaction begins with 10&micro;L of normalized sample to which reagents are added. As such, initial submission of clean samples that have been pre-normalized in greater than 10&micro;L of appropriate buffer will hasten sample processing. 
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:[[IMAGE:Ultra_II_DNA_Input.jpg | 400px]] [[IMAGE:Ultra_II_FAtrace.jpg | 400px]]
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