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BioMicroCenter:BioAnalyzer INFO
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==RNA== The BioAnalyzer analyzes RNA using the same principles as denaturing slab agarose or polyacrylamide gels. A very small tube of gel is created and a fraction of the RNA loaded, along with a low marker, is placed at one end of the chip. Then, using a voltage gradient, the sample is run down the length of the chip. As the RNA passes a laser, it is detected. Subsequent samples are run down the same lane of the chip. Quantification is performed relative to the sizing standards. The BioAnalyzer software can generate the [[BioMicroCenter:RIN|RNA Integrity Number (RIN)]], calculate ribosomal ratios of total RNA samples, and detect ribosomal RNA contamination in mRNA. [http://www.chem.agilent.com/en-us/products/instruments/lab-on-a-chip/2100bioanalyzer/smallrnaassay/pages/default.aspx RNA] - [http://www.chem.agilent.com/en-us/products/instruments/lab-on-a-chip/2100bioanalyzer/rnasolutions/pages/gp38725.aspx Analysis Specifications] ''(Agilent)'' RNA arrays are segregated by the concentration of RNA used and the type of RNAs being analyzed * RNA Nano: 25-500 ng/ul total RNA * RNA Pico: 0.05-5 ng/ul total RNA * small RNA: 0.05-2 ng/ul miRNAs
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