Editing BioMicroCenter:PricingFY2023b (section)

=== ILLUMINA LIBRARY - RNA ===

{| class="wikitable" border=1
 !width=160| ILLUMINA Sample Preparation
 !width=60| Type
 !width=60| Batch Size
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 ! Notes
 |-
 ! colspan="4" | '''STANDARD RNA''' <BR> Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
 ! colspan="3" style="color: blue;" | '''HIGH THROUGHPUT RNA''' <BR> Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included
 |-
 |rowspan="3"|polyA based isolation <BR><BR> Kapa mRNA Hyperprep <BR>OR<BR> NEB UltraII RNAseq
 |align="center"|<B>STD</B>
 |align="center"| per sample
 |align="right"| $157
 |align="right"| $173
 |align="right"| $236
 |Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
 |-
 |rowspan=2 align="center" style="color: blue;"| <B>HTL</b><BR>(NEB only)
 |align="center" style="color: blue;" | ''24''
 |align="right" style="color: blue;" | $1,400
 |align="right" style="color: blue;" | $1,540
 |align="right" style="color: blue;" | $1,960
 |Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
 |-
 |align="center" style="color: blue;" | ''96''
 |align="right" style="color: blue;" | $2,800
 |align="right" style="color: blue;" | $3,080
 |align="right" style="color: blue;" | $3,920
 |Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
 |-
 
 |rowspan="2"| High Throughput 3' Digital Gene Expression (HT3DGE)
 |rowspan=2  align="center" style="color: blue;"| <B>HTL</b>
 |align="center" style="color: blue;"| ''24'' 
 |align="right" style="color: blue;" | $1,050
 |align="right" style="color: blue;" | $1,155
 |align="right" style="color: blue;" | $1,470
 | rowspan="2"  style="color: blue;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
 |-
 |align="center" style="color: blue;" | ''96''
 |align="right" style="color: blue;" | $2,100
 |align="right" style="color: blue;" | $2,310
 |align="right" style="color: blue;" | $2,940
 |-
 
 |rowspan="3"|Total RNA sequencing:Mammalian <BR><BR> Kapa Hyperprep + RiboErase <BR>OR<BR> NEB RNAseq +Ribodepletion
 |align="center"|<B>STD</B>
 |align="center"| per sample
 |align="right"| $235
 |align="right"| $258.5
 |align="right"| $352.5
 |Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control. 
 |-
 |rowspan=2 align="center" style="color: blue;"| <B>HTL</b><BR>(NEB only, covaris RNA fragmentation)
 |align="center" style="color: blue;" | ''24''
 |align="right" style="color: blue;" | $2,400
 |align="right" style="color: blue;" | $2,640
 |align="right" style="color: blue;" | $3,360
 |rowspan=2|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
 |-
 |align="center" style="color: blue;" | ''96''
 |align="right" style="color: blue;" | $4,800
 |align="right" style="color: blue;" | $5,280
 |align="right" style="color: blue;" | $6,720
 |-
 
 |rowspan=3|Low Input polyA+ <BR> Clontech SMARTseq + NexteraXT or Flex
 |align="center"| '''STD'''<BR>v4
 |align="center"|per sample
 |align="right"| $262
 |align="right"| $289
 |align="right"| $394
 | rowspan="3" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
 |-
 |rowspan="2" align="center" style="color: blue;"| '''HTL'''<br>v2
 |align="center" style="color: blue;" | ''24''
 |align="right" style="color: blue;" | $1880
 |align="right" style="color: blue;" | $1980
 |align="right" style="color: blue;" | $2520
 |-
 |align="center" style="color: blue;" | ''96''
 |align="right" style="color: blue;" | $2800
 |align="right" style="color: blue;" | $3080
 |align="right" style="color: blue;" | $3920
 |-
 
 |rowspan="3"|Low Input Mammalian Total <BR> Clontech ZapR Kit
 |align="center"| STD
 |align="center"| per sample
 |align="right"| $210
 |align="right"| $231
 |align="right"| $315
 | rowspan="1" | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA). 
 |-
 |rowspan=2 align="center" style="color: blue;"| <B>HTL</b><BR>(covaris RNA fragmentation)
 |align="center" style="color: blue;" | ''24''
 |align="right" style="color: blue;" | $2200
 |align="right" style="color: blue;" | $2420
 |align="right" style="color: blue;" | $3080
 |rowspan=2|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
 |-
 |align="center" style="color: blue;" | ''96''
 |align="right" style="color: blue;" | $4400
 |align="right" style="color: blue;" | $4840
 |align="right" style="color: blue;" | $6160
 |-
 | Small RNA
 |align="center"| STD
 | align="center"|per sample
 | align="right"|$289
 | align="right"|$318
 | align="right"|$433
 | Uses NEB, BIOO or QIAGEN small RNA kit
 |-
 ! colspan="7" | '''ADDITIONAL FEES''' <BR> Samples not in proper input format will be subject to these additional charges.
 |-
 |colspan=2 style="color: blue;" | High Throughput Setup
 |align="center" style="color: blue;" |48 samples 
 |align="right" style="color: blue;" | $50
 |align="right" style="color: blue;" | $55
 |align="right" style="color: blue;" | $75
 | 
 |-
 |colspan=2|Sample Cleanup
 |align="center"|per sample
 |align="right"| $5
 |align="right"| $5.50
 |align="right"| $7.50
 |SPRI cleanup of samples to remove impurities. 
 |-
 |colspan=2 style="color: blue;" |Quantification
 |align="center" style="color: blue;" |per sample
 |align="right" style="color: blue;" | $6
 |align="right" style="color: blue;" | $6.60
 |align="right" style="color: blue;" | $8.40
 |Quantification of samples (FA) prior to arraying.
 |-
|}

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