Editing BioMicroCenter:RNA LIB (section)

==[https://www.neb.com/products/e7760-nebnext-ultra-ii-directional-rna-library-prep-kit-for-illumina#Product%20Information NEB Ultra II Directional RNA with Poly(A) Selection] ==
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  !Service 
  !Standard RNA Library Prep
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  |SAMPLE INPUT || Intact total RNA ([[BioMicroCenter:RIN|RIN]] 7+) 
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  |RANGE OF INPUT || 10-100 ng
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  |INCLUDED || Initial QC by Fragment Analyzer <BR> Library preparation <BR> Illumina QC
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  |SEQUENCING RECOMMENDATIONS || All platforms
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  |INDEX AVAILABILITY || 112 [[BioMicroCenter:HTLR_TEST_HTLR_TEST#Unique_versus_Combinatorial_Dual_Indexing|Unique Dual Indexes]] <br> 192 [[BioMicroCenter:HTLR_TEST_HTLR_TEST#Unique_versus_Combinatorial_Dual_Indexing|Combinatorial Dual Indexes]]
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  |SUBMISSION || MIT - [https://mit.ilabsolutions.com/service_item/new/3381?spt_id=3863 ilabs] <BR> External - [[BioMicroCenter:Forms|form]]
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  |UNIT || Per sample
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The BioMicro Center utilizes the NEBNext Ultra II Directional RNA preparation with poly(A) selection kit to prepare standard RNA libraries. The selection of mRNA transcripts occurs by hybridization of the poly(A)-tail to magnetic oligo-d(T) beads. We can reliably generate RNA-Seq libraries with inputs ranging from 10ng to 100ng (recommended input of > 50ng). High-quality samples are required because the method is reliant on poly(A)-tails during RNA isolation. We also offer a [[BioMicroCenter:RNA_HTL|high-throughput protocol]].
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