Editing BioMicroCenter:QC (section)

== AATI FRAGMENT ANALYZER ==
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  !Service 
  !AATI FRAGMENT ANALYZER
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  |INPUT || 5uL for standard <br> 2uL/well for preload
  |-
  |SUBMISSION || MIT - [https://mit.ilabsolutions.com/service_item/new/3381?spt_id=4110 ilabs] <BR> External - [[BioMicroCenter:Forms|form]] <BR> Preloaded rows must be in semiskirted plates from the BioMicro Center (or equivalent).
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  |UNIT || Samples or preloaded rows
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  |TURN AROUND || Typically < 1 business day
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  |SAMPLE SUBMISSION || 9a-4:30p normal weekdays. <BR> Samples brought by after 4:30p must be accompanied by cookies.
  |-
  |DONATED BY || Department of Biology
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The [[BioMicroCenter:Advanced_analytical_Fragment_analyzer|Advanced Analytical Fragment Analyzer (FA)]] is a highly efficient and accurate fluorescence-based electrophoresis instrument used to size and quantify DNA and RNA. It is faster and more cost effective than the BioAnalyzer2100, and can handle unsupervised runs of up to 192 samples. Each run takes ~60 minutes and 2 μL of sample.  Users may submit any volume of sample with the expectation that if there is less than 2 μL, the samples will be diluted.  To save money and submit a larger quantity of samples, users may come into the BMC and obtain a special 96 well plate or plates.  For mass submission, 2 μL must be put into each sample well row by row.  There is a way to submit rows in iLabs, but please remember to come and get special plates from the BMC to do mass submission. <BR><BR>

The Fragment analyzer was purchased in 2012 and upgraded to handle 48 samples in a batch in 2017. Fragment analyzer reports a "RIN eQuivalent Number (RQN) instead of RIN.
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[[Image:FA.jpg|right]]
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  ! Assay
  ! Sizing Range
  ! Concentration Range
  |-
  |High Sensitivity DNA
  |50bp - 5kbp
  |Fragment: 5pg-1ng/uL <br> Smear: 50pg-5ng/uL
  |-
  |Nano RNA
  |50bp - 5kbp
  |
  |-
  |Pico RNA (recommend FemtoPulse instead)
  |50bp - 5kbp
  |50pg-5ng/uL
  |}
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