Editing BioMicroCenter:PricingFY2025 (section)

== SEQUENCING ==
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason. <BR><BR>

=== NOVASEQ SEQUENCING ===
{| class="wikitable" border=1 
 ! NOVASEQ SEQUENCING
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=80| unit
 ! Notes
 |-
 |NovaSeq SP 100nt
 |align=right| $3,699
 |align=right| $4,068
 |align=right| $5,178
 |rowspan=9| Per flowcell
 |rowspan=9| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
 |-
 |NovaSeq SP 300nt** 
 |align=right| $5,238
 |align=right| $5,761
 |align=right| $7,333.20
 |-
 |NovaSeq SP 500nt
 |align=right| $7,020
 |align=right| $7,722
 |align=right| $9,828
 |-
 |NovaSeq S1 100nt**
 |align=right| $5,832
 |align=right| $6,415.20
 |align=right| $8,164.80
 |-
 |NovaSeq S1 200nt**
 |align=right| $7,452
 |align=right| $8,197.20
 |align=right| $10,432
 |-
 |NovaSeq S1 300nt**
 |align=right| $8,127
 |align=right| $8,939.70
 |align=right| $11,377.80
 |-
 |NovaSeq S2 100nt**
 |align=right| $10,395
 |align=right| $11,434.50
 |align=right| $14,553
 |-
 |NovaSeq S2 200nt**
 |align=right| $12,960
 |align=right| $14,256
 |align=right| $18,144
 |-
 |NovaSeq S2 300nt**
 |align=right| $13,500
 |align=right| $14,850
 |align=right| $18,900
 |-
 |NovaSeq S4 50nt 
 |align=right| $3,780
 |align=right| $4,158
 |align=right| $5,292
 |rowspan=2| Per Lane
 |rowspan=2| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
 |-
 |NovaSeq S4 300nt
 |align=right| $5,400
 |align=right| $5,940
 |align=right| $7,560
 |-
|}
 ** - minimal inventory maintained

=== NEXTSEQ500 SEQUENCING ===
{| class="wikitable" border=1 
 ! NEXTSEQ SEQUENCING
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=80| unit
 ! Notes
 |-
 |91nt kit <BR> (eg. 40PE or 75SE)
 |align="right"| $2,570
 |align="right"| $2,827
 |align="right"| $3,598
 |align="center" rowspan="3"| per flowcell
 |rowspan="3"| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year 
 |-
 |166nt kit <BR> (eg. 80PE or 150SE)
 |align="right"| $4,375
 |align="right"| $4,812.50
 |align="right"| $6,125
 |-
 |316nt kit <BR> (eg. 150PE or 300SE)
 |align="right"| $6,420
 |align="right"| $7,062
 |align="right"| $8,988
|}

=== MISEQ SEQUENCING ===
{| class="wikitable" border=1 
 ! MISEQ SEQUENCING
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=80| unit
 ! Notes
 |-
 |70nt V2 kit <BR> (eg. 30PE or 70SE)
 |align="right"| $1,320
 |align="right"| $1,452
 |align="right"| $1,848
 |align="center" rowspan=6| per lane
 |rowspan=6| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
 |-
 |150nt V3 kits <BR> (eg. 75PE)
 |align="right"| $1,430
 |align="right"| $1,573
 |align="right"| $2,002
 |-
 |300nt V2 kit<BR> (eg. 150PE)
 |align="right"| $1,595
 |align="right"| $1,754.50
 |align="right"| $2,233
 |-
 |500nt V2 kit <BR> (eg. 250PE)
 |align="right"| $1,760
 |align="right"| $1,936
 |align="right"| $2,464
 |-
 |600nt V3 kit<BR> (eg. 300PE)
 |align="right"| $2,200
 |align="right"| $2,420
 |align="right"| $3,080
 |-
 |500nt '''NANO''' Run <BR> (eg. 250PE)
 |align="right"| $770
 |align="right"| $847
 |align="right"| $1,078
|}

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===
{| class="wikitable" border=1 
 ! SINGULAR G4 SEQUENCING
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=80| unit
 ! Notes
 |-
 |50 PE: F3 Lane (~100m reads)
 |align="right"| $300
 |align="right"| $330
 |align="right"| $420
 |align="center" rowspan=2| per lane
 |rowspan=3| Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
 |-
 |150 PE: F3 Lane (~100m reads)
 |align="right"| $450
 |align="right"| $495
 |align="right"| $630
 |-
 |Rapid 300nt: F3 flowcell (~400m reads)*
 |align="right"| $1800
 |align="right"| $1980
 |align="right"| $2520
 |align="center"| per flowcell
 |-
|}
 * Rapid flowcells are required for adapted Illumina libraries.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI] ===
{| class="wikitable" border=1 
 ! ELEMENT AVITI SEQUENCING
 !width=80| Lane/Flowcell
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=80| unit
 ! Notes
 |-
 |rowspan=2| 75 PE
 | Full flowcell (2 lane, 800m read)
 |align="right"| $1,900
 |align="right"| $2,090
 |align="right"| $2,660
 |align="center"| per flowcell
 |rowspan=5| Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
 |-
 |One lane (~400m read)
 |align="right"| $1,000
 |align="right"| $1,100
 |align="right"| $1,400
 |align="center"| per lane
 |-
 |rowspan=2| 150 PE
 | Full flowcell (2 lane, ~800m read)
 |align="right"| $2,650
 |align="right"| $2,915
 |align="right"| $3,710
 |align="center"| per flowcell
 |-
 |One lane (~400m read)
 |align="right"| $1,375
 |align="right"| $1,513
 |align="right"| $1,925
 |align="center"| per lane
 |-
 | 300 PE
 | Full flowcell (~200m reads)
 |align="right"| $3,500
 |align="right"| $3,850
 |align="right"| $4,900
 |align="center"| per lane
|}

=== OTHER SHORT READ SERVICES ===
{| class="wikitable" border=1 
 ! ILLUMINA SUPPORTING SERVICES
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=80| unit
 ! Notes
 |-
 | Quality Control Only -only one QC is included per sequencing lane.
 |align="right"| $35
 |align="right"| $38.50
 |align="right"| $49
 |align="center"| per sample
 | Includes RT-PCR and Frag.Analyzer
 |-
 | Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling.
 |align="right"| $15
 |align="right"| $16.50
 |align="right"| $21
 |align="center"| per sample
 | Includes Fluorometric quantification and Frag.Analyzer
 |-
 |qPCR only (short read)
 |align="right"| $25
 |align="right"| $27.50
 |align="right"| $35
 |align="center"| per sample
 | Includes qPCR values from standard QC only
 |-
 |qPCR only - full plate(short read)
 |align="right"| $400
 |align="right"| $440
 |align="right"| $560
 |align="center"| per sample
 | excludes column 1 (used for controls)
|}

=== LONG READ SEQUENCING ===

{| class="wikitable" border=1 
 ! PROMETHION SEQUENCING
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=80| unit
 ! Notes
 |-
 | 72 hour Flowcell 
 |align="right"| $1,200
 |align="right"| $1,320
 |align="right"| $1,680
 |align="center"|per Flowcell
 ||Fastq stored for 1y. Trace data stored only 30d.
|}