Editing BioMicroCenter:PricingFY2022 (section)

== SEQUENCING ==
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason. <BR><BR>

''' PRICING UPDATE 7/1/2021 '''

=== NOVASEQ SEQUENCING ===
pricing update effective 10/1/2020
{| class="wikitable" border=1 
 ! NOVASEQ SEQUENCING
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=80| unit
 ! Notes
 |-
 |NovaSeq: 400m reads
 |align=right| $1,600
 |align=right| $1,760
 |align=right| $2,240
 |Per 400m reads (max 150PE)
 |Requires BMC library prep, Max 96 samples
 |-
 |NovaSeq SP 100nt
 |align=right| $3,365
 |align=right| $3,701
 |align=right| $4,711
 |rowspan=9| Per flowcell
 |rowspan=9| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
 |-
 |NovaSeq SP 300nt** 
 |align=right| $5,200
 |align=right| $5,720
 |align=right| $7,280
 |-
 |NovaSeq SP 500nt
 |align=right| $6,400
 |align=right| $7,040
 |align=right| $8,960
 |-
 |NovaSeq S1 100nt**
 |align=right| $5,300
 |align=right| $5,830
 |align=right| $7,420
 |-
 |NovaSeq S1 200nt**
 |align=right| $6,900
 |align=right| $7,590
 |align=right| $9,660
 |-
 |NovaSeq S1 300nt**
 |align=right| $7,750
 |align=right| $8,525
 |align=right| $10,850
 |-
 |NovaSeq S2 100nt**
 |align=right| $9,500
 |align=right| $10,450
 |align=right| $13,300
 |-
 |NovaSeq S2 200nt**
 |align=right| $11,500
 |align=right| $12,650
 |align=right| $16,100
 |-
 |NovaSeq S2 300nt**
 |align=right| $12,600
 |align=right| $13,860
 |align=right| $17,640
 |-
 |NovaSeq S4 50nt 
 |align=right| $3,500
 |align=right| $3,850
 |align=right| $4,900
 |rowspan=2| Per Lane
 |rowspan=2| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
 |-
 |NovaSeq S4 300nt
 |align=right| $5,000
 |align=right| $5,500
 |align=right| $7,000
 |-
|}
** - minimal inventory maintained


=== NEXTSEQ500 SEQUENCING ===
{| class="wikitable" border=1 
 ! NEXTSEQ SEQUENCING
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=80| unit
 ! Notes
 |-
 |91nt kit <BR> (eg. 40PE or 75SE)
 |align="right"| $2,270
 |align="right"| $2,497
 |align="right"| $3,178
 |align="center" rowspan="3"| per flowcell
 |rowspan="3"| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year 
 |-
 |166nt kit <BR> (eg. 80PE or 150SE)
 |align="right"| $3,900
 |align="right"| $4,290
 |align="right"| $5,460
 |-
 |316nt kit <BR> (eg. 150PE or 300SE)
 |align="right"| $5,800
 |align="right"| $6,380
 |align="right"| $8,120
|}

=== MISEQ SEQUENCING ===
{| class="wikitable" border=1 
 ! MISEQ SEQUENCING
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=80| unit
 ! Notes
 |-
 |70nt V2 kit <BR> (eg. 30PE or 70SE)
 |align="right"| $1,250
 |align="right"| $1,375
 |align="right"| $1,750
 |align="center" rowspan=6| per lane
 |rowspan=6| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
 |-
 |150nt V3 kits <BR> (eg. 75PE)
 |align="right"| $1,350
 |align="right"| $1,485
 |align="right"| $1,890
 |-
 |300nt V2 kit<BR> (eg. 150PE)
 |align="right"| $1,475
 |align="right"| $1,623
 |align="right"| $2,100
 |-
 |500nt V2 kit <BR> (eg. 250PE)
 |align="right"| $1,703
 |align="right"| $1,874
 |align="right"| $2,310
 |-
 |600nt V3 kit<BR> (eg. 300PE)
 |align="right"| $2,043
 |align="right"| $2,248
 |align="right"| $2,898
 |-
 |500nt '''NANO''' Run <BR> (eg. 250PE)
 |align="right"| $730
 |align="right"| $803
 |align="right"| $1,022
|}


=== HISEQ2000 SEQUENCING ===
The HiSeq2000 in the BioMicro Center is in the process of being retired. We strongly encourage users to move to one of the other sequencers if possible.<BR>
{| class="wikitable" border=1 
 ! HISEQ SEQUENCING
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=80| unit
 ! Notes
 |-
 |Single End 40nt
 |align="right"| $1,200
 |align="right"| $1,320
 |align="right"| $1,800
 |align="center"| per lane
 | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1y.
 |-
 | Other read lengths
 |colspan="5" align=left |7/1/18: Custom only. Requires purchase of full flowcell.
|}

=== OTHER ILLUMINA SERVICES ===
{| class="wikitable" border=1 
 ! ILLUMINA SUPPORTING SERVICES
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=80| unit
 ! Notes
 |-
 | Quality Control Only -only one QC is included per sequencing lane.
 |align="right"| $20
 |align="right"| $26
 |align="right"| $30
 |align="center"| per sample
 | Includes RT-PCR and Frag.Analyzer
 |-
 | Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling.
 |align="right"| $10
 |align="right"| $13
 |align="right"| $15
 |align="center"| per sample
 | Includes Fluorometric quantification and Frag.Analyzer
 |-
 |qPCR only (Illumina)
 |align="right"| $15
 |align="right"| $16.5
 |align="right"| $22.5
 |align="center"| per sample
 | Includes qPCR values from standard QC only
 |-
 |qPCR only - full plate(Illumina)
 |align="right"| $300
 |align="right"| $330
 |align="right"| $450
 |align="center"| per sample
 | excludes column 1 (used for controls)
|}

=== LONG READ SEQUENCING ===

{| class="wikitable" border=1 
 ! SEQUEL SEQUENCING
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=80| unit
 ! Notes0
 |-
 | 20 hour v3 Chemistry 
 |align="right"| $1,470
 |align="right"| $1,617
 |align="right"| $2,205
 |align="center"| per SMRTCell
 |align="center"| Includes polymerase binding and movie.

 |-
 ! PROMETHION SEQUENCING
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=80| unit
 ! Notes
 |-
 | 72 hour Flowcell 
 |align="right"| $1,500
 |align="right"| $1,650
 |align="right"| $2,100
 |align="center"|per Flowcell
 ||Fastq stored for 1y. Trace data stored only 30d.
|}