Editing BioMicroCenter:PricingFY2015 (section)

== [[BioMicroCenter:Sequencing|ILLUMINA SEQUENCING]] ==

DRAFT - NOT FOR USE

=== SEQUENCING ===
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the HiSeq.

{| border=1 
 ! ILLUMINA SEQUENCING
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=80| unit
 ! Notes
 |-
 | colspan="6" align=center |<BR>'''HiSEQ 2000''' sequencing (minimum 150m reads/lane*. Average ~160-240m reads/lane)
 |-
 | Single End (40nt)
 |align="right"| $1,085
 |align="right"| $1,195
 |align="right"| $1,628
 |align="center"| per lane
 | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1y.
 |-
 | Paired End (40+40nt)
 |align="right"| $1,625
 |align="right"| $1,788
 |align="right"| $2,438
 |align="center"| per lane
 | 
 |-
 | Add'l 40nt
 |align="right"| $300
 |align="right"| $330
 |align="right"| $450
 |align="center"| per lane
 |
 |- 
 | colspan="6" align=center |<BR>'''MiSeq v2''' sequencing (minimum 8m reads/lane. maximum ~15m reads/lane)
 |-
 | 70nt Run
 |align="right"| $1,150
 |align="right"| $1,265
 |align="right"| $1,725
 |align="center"| per lane
 | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
 |-
 | 300nt Run (PE or SE)
 |align="right"| $1,350
 |align="right"| $1,485
 |align="right"| $2,025
 |align="center"| per lane
 | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
 |-
 | 500nt Run (PE or SE)
 |align="right"| $1,600
 |align="right"| $1,760
 |align="right"| $2,400
 |align="center"| per lane
 | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
 |-
 | colspan="6" align=center |<BR>'''MiSeq v3''' sequencing (minimum 15m reads/lane. Expected ~25m reads/lane)
 |-
 | 600nt Run (PE or SE)
 |align="right"| $2,050
 |align="right"| $2,255
 |align="right"| $3,075
 |align="center"| per lane
 | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
 |-
 | colspan="6" align=center |<BR>'''GAII / GAIIx''' sequencing (Average ~25-40m reads/lane)
 |-
 | colspan="6" align=center |GAII Services are being phased out in FY2015. Requests that must be processed on the GAII will be priced per experiment.
 |-
 | colspan="6" align=center |<BR>'''Additional Services''' 
 |- 
 | colspan="6" align=center |''These services apply to samples submitted for all platforms.''
 |- 
 | Sample Multiplexing
 |align="right"| $50
 |align="right"| $55
 |align="right"| $75
 |align="center"| per 10 samples
 | Combined over the whole order. Only applies to samples combined by BMC technicians.
 |-
 | Add'l Repriming (HiSeq only)
 |align="right"| $200
 |align="right"| $220
 |align="right"| $300
 |align="center"| per flow cell
 | Uses 6-8nt. Cost is divided by number of lanes requiring repriming. Repriming is required for standard Illumina multiplexing.
 |-
 | Quality Control Only
 |align="right"| $25
 |align="right"| $28
 |align="right"| $38
 |align="center"| per sample
 | Includes RT-PCR and BioAnalyzer
|}

<BR><BR>
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.''<BR>


=== SAMPLE PREPARATION ===

{| border=1 
 ! ILLUMINA Sample Preparation
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=80| unit
 ! Notes
 |-
 | colspan="6" | '''STARTING WITH DNA''' 
 |-
 | Full Service Sheared DNA (SPRIWORKS)
 |align="right"| $150
 |align="right"| $165
 |align="right"| $225
 |align="center"| per sample
 | Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
 |-
 | ''SPRI-TE use only''
 |align="right"| $100
 |align="right"| $110
 |align="right"| N/A
 |align="center"| per sample
 | User must provide all primers. Samples will be returned for amplification.
 |-
 | Intact Genomic DNA (NEXTERA) - 50ng input
 |align="right"| $175
 |align="right"| $193
 |align="right"| $263
 |align="center"| per sample
 | Includes clean up, tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
 |-
 | Low Input Intact Genomic DNA (NEXTERA-XT) - 1ng input
 |align="right"| $100
 |align="right"| $110
 |align="right"| $150
 |align="center"| per sample
 | Includes tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). 
 |-
 | 16S Amplicon
 |align="right"| $1,200
 |align="right"| $1,320
 |align="right"| $1,800
 |align="center"| per 48 sample
 | Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer). 
 |-
 | Other Amplicon
 |align="right"| $960
 |align="right"| $1,056
 |align="right"| $1,440
 |align="center"| per 48 sample
 | Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer). <BR> Samples *must* be submitted amplified with appropriate linkers.
 |-
 | colspan="6" | '''STARTING WITH RNA''' 
 |-
 | Illumina TruSeq 
 |align="right"| $300
 |align="right"| $330
 |align="right"| $450
 |align="center"| per sample
 | Preformed using TruSeq AND SPRIworks system. <br> Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
 |-
 |''+strand specific''
 |align="right"| $30
 |align="right"| $33
 |align="right"| $45
 |align="center"| per sample
 | Requires 1ug of RNA. Uses the dUTP method.
 |-
 | Epicenter Ribozero<br>''no polyA pulldown''
 |align="right"| $350
 |align="right"| $385
 |align="right"| $525
 |align="center"| per sample
 | Requires 1ug of RNA. Based on Illumina Truseq chemistry
 |-
 | Low Input (Clontech)
 |align="right"| $400
 |align="right"| $440
 |align="right"| $600
 |align="center"| per sample
 | Can work on single cells
 |-
 | Low Input (NuGen)
 |align="right"| $475
 |align="right"| $523
 |align="right"| $713
 |align="center"| per sample
 | Can work on degraded RNA.
 |-
 | Tight Size Cut
 |align="right"| $100
 |align="right"| $110
 |align="right"| $150
 |align="center"| per pool
 | +/- 40bp using PippinPrep
 |-
 | Small RNA
 | align="right"|$275
 | align="right"|$303
 | align="right"|$413
 | align="center"|per sample
 | Uses NEB small RNA kit
 |-
 | colspan="6" | '''STARTING WITH CHROMATIN''' Note: User must supply antibodies.
 |-
 | Full Service ChIP-seq
 |align="right"| $210
 |align="right"| $231
 |align="right"| $315
 |align="center"| per sample
 | Preformed using IP-Star AND SPRIworks system. <br> Includes ChIP, library contruction and barcoding.
 |-
 | ''IP-Star Only''
 |align="right"| $300
 |align="right"| $330
 |align="right"| $450
 |align="center"| per batch (1-8 samples)
 | Preformed using IP-Star
 |-
|}

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